eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and ba...
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MDPI AG
2023-01-01
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Series: | Microorganisms |
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Online Access: | https://www.mdpi.com/2076-2607/11/2/332 |
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author | Iztok Dogsa Rok Kostanjšek David Stopar |
author_facet | Iztok Dogsa Rok Kostanjšek David Stopar |
author_sort | Iztok Dogsa |
collection | DOAJ |
description | The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth. |
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format | Article |
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institution | Directory Open Access Journal |
issn | 2076-2607 |
language | English |
last_indexed | 2024-03-11T08:24:25Z |
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publisher | MDPI AG |
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series | Microorganisms |
spelling | doaj.art-998a5972689f40c6a05016631e38eae32023-11-16T22:14:25ZengMDPI AGMicroorganisms2076-26072023-01-0111233210.3390/microorganisms11020332eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton SuspensionIztok Dogsa0Rok Kostanjšek1David Stopar2Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaThe self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth.https://www.mdpi.com/2076-2607/11/2/332autoaggregates<i>B. subtilis</i>planktoneDNAbiofilm |
spellingShingle | Iztok Dogsa Rok Kostanjšek David Stopar eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension Microorganisms autoaggregates <i>B. subtilis</i> plankton eDNA biofilm |
title | eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension |
title_full | eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension |
title_fullStr | eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension |
title_full_unstemmed | eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension |
title_short | eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension |
title_sort | edna provides a scaffold for autoaggregation of i b subtilis i in bacterioplankton suspension |
topic | autoaggregates <i>B. subtilis</i> plankton eDNA biofilm |
url | https://www.mdpi.com/2076-2607/11/2/332 |
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