eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension

The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and ba...

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Main Authors: Iztok Dogsa, Rok Kostanjšek, David Stopar
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/11/2/332
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author Iztok Dogsa
Rok Kostanjšek
David Stopar
author_facet Iztok Dogsa
Rok Kostanjšek
David Stopar
author_sort Iztok Dogsa
collection DOAJ
description The self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth.
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spelling doaj.art-998a5972689f40c6a05016631e38eae32023-11-16T22:14:25ZengMDPI AGMicroorganisms2076-26072023-01-0111233210.3390/microorganisms11020332eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton SuspensionIztok Dogsa0Rok Kostanjšek1David Stopar2Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaBiotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, SloveniaThe self-binding of bacterial cells, or autoaggregation, is, together with surface colonization, one of the first steps in the formation of a mature biofilm. In this work, the autoaggregation of B. subtilis in dilute bacterial suspensions was studied. The dynamics of cell lysis, eDNA release, and bacterial autoaggregate assembly were determined and related to the spatial autocorrelation of bacterial cells in dilute planktonic bacterial suspensions. The non-random distribution of cells was associated with an eDNA network, which stabilized the initial bacterial cell-cell aggregates. Upon the addition of DNase I, the aggregates were dispersed. The release of eDNA during cell lysis allows for the entrapment of bacterial drifters at a radius several times the size of the dying bacteria. The size of bacterial aggregates increased from 2 to about 100 μm in diameter in dilute bacterial suspensions. The results suggest that B. subtilis cells form previously unnoticed continuum of autoaggregate structures during planktonic growth.https://www.mdpi.com/2076-2607/11/2/332autoaggregates<i>B. subtilis</i>planktoneDNAbiofilm
spellingShingle Iztok Dogsa
Rok Kostanjšek
David Stopar
eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
Microorganisms
autoaggregates
<i>B. subtilis</i>
plankton
eDNA
biofilm
title eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
title_full eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
title_fullStr eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
title_full_unstemmed eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
title_short eDNA Provides a Scaffold for Autoaggregation of <i>B. subtilis</i> in Bacterioplankton Suspension
title_sort edna provides a scaffold for autoaggregation of i b subtilis i in bacterioplankton suspension
topic autoaggregates
<i>B. subtilis</i>
plankton
eDNA
biofilm
url https://www.mdpi.com/2076-2607/11/2/332
work_keys_str_mv AT iztokdogsa ednaprovidesascaffoldforautoaggregationofibsubtilisiinbacterioplanktonsuspension
AT rokkostanjsek ednaprovidesascaffoldforautoaggregationofibsubtilisiinbacterioplanktonsuspension
AT davidstopar ednaprovidesascaffoldforautoaggregationofibsubtilisiinbacterioplanktonsuspension