Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers?
The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo−maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics,...
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MDPI AG
2020-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/2/419 |
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| author | Katrien Smits Yannick Gansemans Laurentijn Tilleman Filip Van Nieuwerburgh Margot Van De Velde Ilse Gerits Cyrillus Ververs Kim Roels Jan Govaere Luc Peelman Dieter Deforce Ann Van Soom |
| author_facet | Katrien Smits Yannick Gansemans Laurentijn Tilleman Filip Van Nieuwerburgh Margot Van De Velde Ilse Gerits Cyrillus Ververs Kim Roels Jan Govaere Luc Peelman Dieter Deforce Ann Van Soom |
| author_sort | Katrien Smits |
| collection | DOAJ |
| description | The signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo−maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics, transcriptomics and miRNA expression can be linked directly is currently lacking. The aim of this study was to provide such analysis. Endometrial biopsies, uterine fluid, embryonic tissues, and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples, mRNA-Sequencing on the same tissue samples and mass spectrometry was conducted previously on the same fluid samples. Differential expression of miRNA, mRNA and proteins showed high conformity with literature and confirmed involvement in pregnancy establishment, embryo quality, steroid synthesis and prostaglandin regulation, but the link between differential miRNAs and their targets was limited and did not indicate the identity of an unequivocal signal for MRP in the horse. Differential expression at the embryo−maternal interface was prominent, highlighting a potential role of miRNAs in embryo−maternal communication during early pregnancy in the horse. These data provide a strong basis for future targeted studies. |
| first_indexed | 2024-04-13T14:30:45Z |
| format | Article |
| id | doaj.art-998a77c3753f4e9db4fdabe962c5c5e3 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T14:30:45Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-998a77c3753f4e9db4fdabe962c5c5e32022-12-22T02:43:11ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121241910.3390/ijms21020419ijms21020419Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers?Katrien Smits0Yannick Gansemans1Laurentijn Tilleman2Filip Van Nieuwerburgh3Margot Van De Velde4Ilse Gerits5Cyrillus Ververs6Kim Roels7Jan Govaere8Luc Peelman9Dieter Deforce10Ann Van Soom11Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumLaboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Gent, BelgiumLaboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Gent, BelgiumLaboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Gent, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumAnimal Genetics Lab, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, 9820 Merelbeke, BelgiumLaboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Gent, BelgiumDepartment of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, BelgiumThe signal for maternal recognition of pregnancy (MRP) has still not been identified in the horse. High-throughput molecular biology at the embryo−maternal interface has substantially contributed to the knowledge on pathways affected during MRP, but an integrated study in which proteomics, transcriptomics and miRNA expression can be linked directly is currently lacking. The aim of this study was to provide such analysis. Endometrial biopsies, uterine fluid, embryonic tissues, and yolk sac fluid were collected 13 days after ovulation during pregnant and control cycles from the same mares. Micro-RNA-Sequencing was performed on all collected samples, mRNA-Sequencing on the same tissue samples and mass spectrometry was conducted previously on the same fluid samples. Differential expression of miRNA, mRNA and proteins showed high conformity with literature and confirmed involvement in pregnancy establishment, embryo quality, steroid synthesis and prostaglandin regulation, but the link between differential miRNAs and their targets was limited and did not indicate the identity of an unequivocal signal for MRP in the horse. Differential expression at the embryo−maternal interface was prominent, highlighting a potential role of miRNAs in embryo−maternal communication during early pregnancy in the horse. These data provide a strong basis for future targeted studies.https://www.mdpi.com/1422-0067/21/2/419maternal recognition of pregnancyhorserna-sequencingproteomicsmicrorna |
| spellingShingle | Katrien Smits Yannick Gansemans Laurentijn Tilleman Filip Van Nieuwerburgh Margot Van De Velde Ilse Gerits Cyrillus Ververs Kim Roels Jan Govaere Luc Peelman Dieter Deforce Ann Van Soom Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers? International Journal of Molecular Sciences maternal recognition of pregnancy horse rna-sequencing proteomics microrna |
| title | Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers? |
| title_full | Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers? |
| title_fullStr | Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers? |
| title_full_unstemmed | Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers? |
| title_short | Maternal Recognition of Pregnancy in the Horse: Are MicroRNAs the Secret Messengers? |
| title_sort | maternal recognition of pregnancy in the horse are micrornas the secret messengers |
| topic | maternal recognition of pregnancy horse rna-sequencing proteomics microrna |
| url | https://www.mdpi.com/1422-0067/21/2/419 |
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