PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis
Alterations to the programmed cell death protein-1 (PD-1) pathway were previously shown to be involved in a poorer prognosis for patients with proliferative glomerulonephritis (PGN). Here, we investigated the association between several infectious agents and the expression of PD-1 and its ligand (PD...
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/21/8001 |
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| author | Ewelina Grywalska Iwona Smarz-Widelska Izabela Korona-Głowniak Sebastian Mertowski Krzysztof Gosik Anna Hymos Jarosław Ludian Paulina Niedźwiedzka-Rystwej Jacek Roliński Wojciech Załuska |
| author_facet | Ewelina Grywalska Iwona Smarz-Widelska Izabela Korona-Głowniak Sebastian Mertowski Krzysztof Gosik Anna Hymos Jarosław Ludian Paulina Niedźwiedzka-Rystwej Jacek Roliński Wojciech Załuska |
| author_sort | Ewelina Grywalska |
| collection | DOAJ |
| description | Alterations to the programmed cell death protein-1 (PD-1) pathway were previously shown to be involved in a poorer prognosis for patients with proliferative glomerulonephritis (PGN). Here, we investigated the association between several infectious agents and the expression of PD-1 and its ligand (PD-L1) on T and B lymphocytes in patients with PGN and nonproliferative glomerulonephritis (NPGN). A cohort of 45 newly-diagnosed patients (23 with PGN and 22 with NPGN) and 20 healthy volunteers was enrolled. The percentage of peripheral blood mononuclear cells expressing PD-1 and PD-L1 antigens was determined by flow cytometry. We found PD-1 and PD-L1 expression on T and B lymphocytes was higher in PGN patients than in NPGN patients and controls. We also found that reactivation of the Epstein-Barr virus (EBV) correlated with the expression of PD-1/PD-L1 antigens in patients with PGN. Further receiver operating characteristic analysis indicated that PD-1 expression could distinguish EBV-positive PGN patients from those with NPGN or healthy controls. The use of PD-1 expression as a non-invasive marker of PGN should be further investigated. |
| first_indexed | 2024-03-10T15:18:01Z |
| format | Article |
| id | doaj.art-99905b115ecc41a2bdbae16e829f2b74 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:18:01Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-99905b115ecc41a2bdbae16e829f2b742023-11-20T18:46:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012121800110.3390/ijms21218001PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative GlomerulonephritisEwelina Grywalska0Iwona Smarz-Widelska1Izabela Korona-Głowniak2Sebastian Mertowski3Krzysztof Gosik4Anna Hymos5Jarosław Ludian6Paulina Niedźwiedzka-Rystwej7Jacek Roliński8Wojciech Załuska9Department of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Nephrology, Cardinal Stefan Wyszynski Provincial Hospital in Lublin, 20-718 Lublin, PolandDepartment of Pharmaceutical Microbiology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Otolaryngology and Laryngeal Oncology, Medical University of Lublin, 20-954 Lublin, PolandDepartment of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandInstitute of Biology, University of Szczecin, 71-412 Szczecin, PolandDepartment of Clinical Immunology and Immunotherapy, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Nephrology, Medical University of Lublin, 20-954 Lublin, PolandAlterations to the programmed cell death protein-1 (PD-1) pathway were previously shown to be involved in a poorer prognosis for patients with proliferative glomerulonephritis (PGN). Here, we investigated the association between several infectious agents and the expression of PD-1 and its ligand (PD-L1) on T and B lymphocytes in patients with PGN and nonproliferative glomerulonephritis (NPGN). A cohort of 45 newly-diagnosed patients (23 with PGN and 22 with NPGN) and 20 healthy volunteers was enrolled. The percentage of peripheral blood mononuclear cells expressing PD-1 and PD-L1 antigens was determined by flow cytometry. We found PD-1 and PD-L1 expression on T and B lymphocytes was higher in PGN patients than in NPGN patients and controls. We also found that reactivation of the Epstein-Barr virus (EBV) correlated with the expression of PD-1/PD-L1 antigens in patients with PGN. Further receiver operating characteristic analysis indicated that PD-1 expression could distinguish EBV-positive PGN patients from those with NPGN or healthy controls. The use of PD-1 expression as a non-invasive marker of PGN should be further investigated.https://www.mdpi.com/1422-0067/21/21/8001Epstein–Barr virusEBVglomerulonephritisimmunologyCD4+ T lymphocyteCD8+ T lymphocyte |
| spellingShingle | Ewelina Grywalska Iwona Smarz-Widelska Izabela Korona-Głowniak Sebastian Mertowski Krzysztof Gosik Anna Hymos Jarosław Ludian Paulina Niedźwiedzka-Rystwej Jacek Roliński Wojciech Załuska PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis International Journal of Molecular Sciences Epstein–Barr virus EBV glomerulonephritis immunology CD4+ T lymphocyte CD8+ T lymphocyte |
| title | PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis |
| title_full | PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis |
| title_fullStr | PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis |
| title_full_unstemmed | PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis |
| title_short | PD-1 and PD-L1 Expression on Circulating Lymphocytes as a Marker of Epstein-Barr Virus Reactivation-Associated Proliferative Glomerulonephritis |
| title_sort | pd 1 and pd l1 expression on circulating lymphocytes as a marker of epstein barr virus reactivation associated proliferative glomerulonephritis |
| topic | Epstein–Barr virus EBV glomerulonephritis immunology CD4+ T lymphocyte CD8+ T lymphocyte |
| url | https://www.mdpi.com/1422-0067/21/21/8001 |
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