Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis
Introduction: In the development of atherosclerosis, the remodeling of blood vessels is a key process involving plaque formation and rupture. So far, most reports mainly believe that macrophages, smooth muscle cells, and endothelial cells located at the intima and media of artery play the key role i...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1118239/full |
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| author | Xiuli Shao Xiuyang Hou Xiaolin Zhang Ruijia Zhang Rongli Zhu He Qi Jianling Zheng Xiaoling Guo Rui Feng |
| author_facet | Xiuli Shao Xiuyang Hou Xiaolin Zhang Ruijia Zhang Rongli Zhu He Qi Jianling Zheng Xiaoling Guo Rui Feng |
| author_sort | Xiuli Shao |
| collection | DOAJ |
| description | Introduction: In the development of atherosclerosis, the remodeling of blood vessels is a key process involving plaque formation and rupture. So far, most reports mainly believe that macrophages, smooth muscle cells, and endothelial cells located at the intima and media of artery play the key role in this process. Few studies had focused on whether fibroblasts located at adventitia are involved in regulating disease process.Methods and results: In this study, we conducted in-depth analysis of single-cell RNA-seq data of the total of 18 samples from healthy and atherosclerotic arteries. This study combines several analysis methods including transcription regulator network, cell-cell communication network, pseudotime trajectory, gene set enrichment analysis, and differential expression analysis. We found that SERPINF1 is highly expressed in fibroblasts and is involved in the regulation of various signaling pathways.Conclusion: Our research reveals a potential mechanism of atherosclerosis, SERPINF1 regulates the formation and rupture of plaques through the Jak-STAT signaling pathway, which may provide new insights into the pathological study of disease. Moreover, we suggest that SRGN and IGKC as potential biomarkers for unstable arterial plaques. |
| first_indexed | 2024-04-09T19:12:40Z |
| format | Article |
| id | doaj.art-99929df5257a41288fef94a46ed381b5 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-09T19:12:40Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-99929df5257a41288fef94a46ed381b52023-04-06T13:11:18ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-03-011410.3389/fphys.2023.11182391118239Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosisXiuli Shao0Xiuyang Hou1Xiaolin Zhang2Ruijia Zhang3Rongli Zhu4He Qi5Jianling Zheng6Xiaoling Guo7Rui Feng8Department of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, ChinaDepartment of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, ChinaDepartment of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, ChinaDepartment of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, ChinaDepartment of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, ChinaDepartment of Medical Biotechnology, Liaoning Vocational College of Medicine, Shenyang, ChinaDepartment of Medical Biotechnology, Liaoning Vocational College of Medicine, Shenyang, ChinaCenter of Scientific Research, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang, ChinaIntroduction: In the development of atherosclerosis, the remodeling of blood vessels is a key process involving plaque formation and rupture. So far, most reports mainly believe that macrophages, smooth muscle cells, and endothelial cells located at the intima and media of artery play the key role in this process. Few studies had focused on whether fibroblasts located at adventitia are involved in regulating disease process.Methods and results: In this study, we conducted in-depth analysis of single-cell RNA-seq data of the total of 18 samples from healthy and atherosclerotic arteries. This study combines several analysis methods including transcription regulator network, cell-cell communication network, pseudotime trajectory, gene set enrichment analysis, and differential expression analysis. We found that SERPINF1 is highly expressed in fibroblasts and is involved in the regulation of various signaling pathways.Conclusion: Our research reveals a potential mechanism of atherosclerosis, SERPINF1 regulates the formation and rupture of plaques through the Jak-STAT signaling pathway, which may provide new insights into the pathological study of disease. Moreover, we suggest that SRGN and IGKC as potential biomarkers for unstable arterial plaques.https://www.frontiersin.org/articles/10.3389/fphys.2023.1118239/fullscRNA-seqatherosclerosisfibroblastsfibrosisremodeling |
| spellingShingle | Xiuli Shao Xiuyang Hou Xiaolin Zhang Ruijia Zhang Rongli Zhu He Qi Jianling Zheng Xiaoling Guo Rui Feng Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis Frontiers in Physiology scRNA-seq atherosclerosis fibroblasts fibrosis remodeling |
| title | Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis |
| title_full | Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis |
| title_fullStr | Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis |
| title_full_unstemmed | Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis |
| title_short | Integrated single-cell RNA-seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis |
| title_sort | integrated single cell rna seq analysis reveals the vital cell types and dynamic development signature of atherosclerosis |
| topic | scRNA-seq atherosclerosis fibroblasts fibrosis remodeling |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1118239/full |
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