Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field
Abstract The H9N2 subtype avian influenza virus (AIV) inactivated vaccine has been used extensively in poultry farms, but it often fails to stimulate a sufficiently high immune response in poultry in the field, although it works well in laboratory experiments; hence, the virus still causes economic...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | Animal Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s44149-022-00040-0 |
| _version_ | 1828344471567204352 |
|---|---|
| author | Xue Pan Xin Su Pingyun Ding Jinhua Zhao Hongrui Cui Dawei Yan Qiaoyang Teng Xuesong Li Nancy Beerens Haitao Zhang Qinfang Liu Mart C. M. de Jong Zejun Li |
| author_facet | Xue Pan Xin Su Pingyun Ding Jinhua Zhao Hongrui Cui Dawei Yan Qiaoyang Teng Xuesong Li Nancy Beerens Haitao Zhang Qinfang Liu Mart C. M. de Jong Zejun Li |
| author_sort | Xue Pan |
| collection | DOAJ |
| description | Abstract The H9N2 subtype avian influenza virus (AIV) inactivated vaccine has been used extensively in poultry farms, but it often fails to stimulate a sufficiently high immune response in poultry in the field, although it works well in laboratory experiments; hence, the virus still causes economic damage every year and poses a potential threat to public health. Based on surveillance data collected in the field, we found that broilers with high levels of maternal-derived antibodies (MDAs) against H9N2 virus did not produce high levels of antibodies after vaccination with a commercial H9N2 inactivated vaccine. In contrast, specific pathogen-free (SPF) chickens without MDAs responded efficiently to that vaccination. When MDAs were mimicked by administering passively transferred antibodies (PTAs) into SPF chickens in the laboratory, similar results were observed: H9N2-specific PTAs inhibited humoral immunity against the H9N2 inactivated vaccine, suggesting that H9N2-specific MDAs might hinder the generation of antibodies when H9N2 inactivated vaccine was used. After challenge with homologous H9N2 virus, the virus was detected in oropharyngeal swabs of the vaccinated and unvaccinated chickens with PTAs but not in the vaccinated chickens without PTAs, indicating that H9N2-specific MDAs were indeed one of the reasons for H9N2 inactivated vaccine failure in the field. When different titers of PTAs were used to mimic MDAs in SPF chickens, high (HI = 12 log2) and medium (HI = log 9 log2) titers of PTAs reduced the generation of H9N2-specific antibodies after the first vaccination, but a booster dose would induce a high and faster humoral immune response even of PTA interference. This study strongly suggested that high or medium titers of MDAs might explain H9N2 inactivated vaccine failure in the field. |
| first_indexed | 2024-04-13T23:57:40Z |
| format | Article |
| id | doaj.art-99933af825674e41a3102bc0c171bcda |
| institution | Directory Open Access Journal |
| issn | 2731-0442 |
| language | English |
| last_indexed | 2024-04-13T23:57:40Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Diseases |
| spelling | doaj.art-99933af825674e41a3102bc0c171bcda2022-12-22T02:23:49ZengBMCAnimal Diseases2731-04422022-05-01211910.1186/s44149-022-00040-0Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the fieldXue Pan0Xin Su1Pingyun Ding2Jinhua Zhao3Hongrui Cui4Dawei Yan5Qiaoyang Teng6Xuesong Li7Nancy Beerens8Haitao Zhang9Qinfang Liu10Mart C. M. de Jong11Zejun Li12Shanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesShanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesBiotechnology Research laboratory, Jiangsu Lihua Animal Husbandry Co. Ltd.Biotechnology Research laboratory, Jiangsu Lihua Animal Husbandry Co. Ltd.Shanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesShanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesShanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesShanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesWageningen Bioveterinary Research, Wageningen University & ResearchBiotechnology Research laboratory, Jiangsu Lihua Animal Husbandry Co. Ltd.Shanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesQuantitative Veterinary Epidemiology, Animal Sciences Group, Wageningen University & ResearchShanghai Veterinary Research Institute, Chinese Academy of Agriculture SciencesAbstract The H9N2 subtype avian influenza virus (AIV) inactivated vaccine has been used extensively in poultry farms, but it often fails to stimulate a sufficiently high immune response in poultry in the field, although it works well in laboratory experiments; hence, the virus still causes economic damage every year and poses a potential threat to public health. Based on surveillance data collected in the field, we found that broilers with high levels of maternal-derived antibodies (MDAs) against H9N2 virus did not produce high levels of antibodies after vaccination with a commercial H9N2 inactivated vaccine. In contrast, specific pathogen-free (SPF) chickens without MDAs responded efficiently to that vaccination. When MDAs were mimicked by administering passively transferred antibodies (PTAs) into SPF chickens in the laboratory, similar results were observed: H9N2-specific PTAs inhibited humoral immunity against the H9N2 inactivated vaccine, suggesting that H9N2-specific MDAs might hinder the generation of antibodies when H9N2 inactivated vaccine was used. After challenge with homologous H9N2 virus, the virus was detected in oropharyngeal swabs of the vaccinated and unvaccinated chickens with PTAs but not in the vaccinated chickens without PTAs, indicating that H9N2-specific MDAs were indeed one of the reasons for H9N2 inactivated vaccine failure in the field. When different titers of PTAs were used to mimic MDAs in SPF chickens, high (HI = 12 log2) and medium (HI = log 9 log2) titers of PTAs reduced the generation of H9N2-specific antibodies after the first vaccination, but a booster dose would induce a high and faster humoral immune response even of PTA interference. This study strongly suggested that high or medium titers of MDAs might explain H9N2 inactivated vaccine failure in the field.https://doi.org/10.1186/s44149-022-00040-0Maternal-derived antibodies (MDAs)Passively transferred antibodies (PTAs)Humoral immune responseVaccination failureH9N2 avian influenza virus (AIV) |
| spellingShingle | Xue Pan Xin Su Pingyun Ding Jinhua Zhao Hongrui Cui Dawei Yan Qiaoyang Teng Xuesong Li Nancy Beerens Haitao Zhang Qinfang Liu Mart C. M. de Jong Zejun Li Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field Animal Diseases Maternal-derived antibodies (MDAs) Passively transferred antibodies (PTAs) Humoral immune response Vaccination failure H9N2 avian influenza virus (AIV) |
| title | Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field |
| title_full | Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field |
| title_fullStr | Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field |
| title_full_unstemmed | Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field |
| title_short | Maternal-derived antibodies hinder the antibody response to H9N2 AIV inactivated vaccine in the field |
| title_sort | maternal derived antibodies hinder the antibody response to h9n2 aiv inactivated vaccine in the field |
| topic | Maternal-derived antibodies (MDAs) Passively transferred antibodies (PTAs) Humoral immune response Vaccination failure H9N2 avian influenza virus (AIV) |
| url | https://doi.org/10.1186/s44149-022-00040-0 |
| work_keys_str_mv | AT xuepan maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT xinsu maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT pingyunding maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT jinhuazhao maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT hongruicui maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT daweiyan maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT qiaoyangteng maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT xuesongli maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT nancybeerens maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT haitaozhang maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT qinfangliu maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT martcmdejong maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield AT zejunli maternalderivedantibodieshindertheantibodyresponsetoh9n2aivinactivatedvaccineinthefield |