Label-free and washing-free alkaline phosphatase assay using a personal glucose meter
Abstract We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13036-019-0182-3 |
| _version_ | 1818937618640928768 |
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| author | Jun Ki Ahn Hyo Yong Kim Chang Yeol Lee Ki Soo Park Hyun Gyu Park |
| author_facet | Jun Ki Ahn Hyo Yong Kim Chang Yeol Lee Ki Soo Park Hyun Gyu Park |
| author_sort | Jun Ki Ahn |
| collection | DOAJ |
| description | Abstract We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In principle, the presence of target ALP scavenges on adenosine 5′-triphosphate (ATP), a phosphate source for hexokinase-catalyzed reactions, and thus suppresses the ensuing cascade enzymatic reactions. As a result, the initial high amount of glucose is maintained and the amount of glucose, which is proportional to ALP activity, is simply measured by a hand-held PGM. Based on this novel strategy, we successfully determined the ALP activity down to 8.9 U/L with the high selectivity. In addition, the diagnostic capability of this method was demonstrated by reliably assaying the ALP activity in non-diluted human blood without any pretreatment steps. |
| first_indexed | 2024-12-20T05:54:50Z |
| format | Article |
| id | doaj.art-9995c05391584063a1f9a7608b0d8012 |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-12-20T05:54:50Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-9995c05391584063a1f9a7608b0d80122022-12-21T19:51:05ZengBMCJournal of Biological Engineering1754-16112019-06-011311510.1186/s13036-019-0182-3Label-free and washing-free alkaline phosphatase assay using a personal glucose meterJun Ki Ahn0Hyo Yong Kim1Chang Yeol Lee2Ki Soo Park3Hyun Gyu Park4Department of Chemical and Biomolecular Engineering (BK21+ Program)Department of Chemical and Biomolecular Engineering (BK21+ Program)Department of Chemical and Biomolecular Engineering (BK21+ Program)Department of Biological Engineering, College of Engineering, Konkuk UniversityDepartment of Chemical and Biomolecular Engineering (BK21+ Program)Abstract We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose. In principle, the presence of target ALP scavenges on adenosine 5′-triphosphate (ATP), a phosphate source for hexokinase-catalyzed reactions, and thus suppresses the ensuing cascade enzymatic reactions. As a result, the initial high amount of glucose is maintained and the amount of glucose, which is proportional to ALP activity, is simply measured by a hand-held PGM. Based on this novel strategy, we successfully determined the ALP activity down to 8.9 U/L with the high selectivity. In addition, the diagnostic capability of this method was demonstrated by reliably assaying the ALP activity in non-diluted human blood without any pretreatment steps.http://link.springer.com/article/10.1186/s13036-019-0182-3Alkaline phosphataseAdenosine 5′-triphosphatePersonal glucose meterCascade enzymatic reactionBiosensor |
| spellingShingle | Jun Ki Ahn Hyo Yong Kim Chang Yeol Lee Ki Soo Park Hyun Gyu Park Label-free and washing-free alkaline phosphatase assay using a personal glucose meter Journal of Biological Engineering Alkaline phosphatase Adenosine 5′-triphosphate Personal glucose meter Cascade enzymatic reaction Biosensor |
| title | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
| title_full | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
| title_fullStr | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
| title_full_unstemmed | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
| title_short | Label-free and washing-free alkaline phosphatase assay using a personal glucose meter |
| title_sort | label free and washing free alkaline phosphatase assay using a personal glucose meter |
| topic | Alkaline phosphatase Adenosine 5′-triphosphate Personal glucose meter Cascade enzymatic reaction Biosensor |
| url | http://link.springer.com/article/10.1186/s13036-019-0182-3 |
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