Label-free and washing-free alkaline phosphatase assay using a personal glucose meter

Label-free and washing-free alkaline phosphatase assay using a personal glucose meter

Abstract We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted by hexokinase and pyruvate kinase to couple ALP activity with the amount of glucose...

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Bibliographic Details
Main Authors: Jun Ki Ahn, Hyo Yong Kim, Chang Yeol Lee, Ki Soo Park, Hyun Gyu Park
Format: Article
Language:English
Published: BMC 2019-06-01
Series:Journal of Biological Engineering
Subjects:
Alkaline phosphatase
Adenosine 5′-triphosphate
Personal glucose meter
Cascade enzymatic reaction
Biosensor
Online Access:http://link.springer.com/article/10.1186/s13036-019-0182-3

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