Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo
Abstract Kaempferol has demonstrated notable positive effects on the osteogenic differentiation of mesenchymal stem cells (MSC) and osteoblasts. A substantial body of research has emphasized the role of dislodged titanium particles in aseptic loosening following joint replacement surgery. This study...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | European Journal of Medical Research |
| Online Access: | https://doi.org/10.1186/s40001-023-01469-w |
| _version_ | 1797630324458389504 |
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| author | Wenkui Qiu Zhenghui Li Zhenyan Su Lichao Cao Lei Li Xi Chen Wanhong Zhang Yanqing Li |
| author_facet | Wenkui Qiu Zhenghui Li Zhenyan Su Lichao Cao Lei Li Xi Chen Wanhong Zhang Yanqing Li |
| author_sort | Wenkui Qiu |
| collection | DOAJ |
| description | Abstract Kaempferol has demonstrated notable positive effects on the osteogenic differentiation of mesenchymal stem cells (MSC) and osteoblasts. A substantial body of research has emphasized the role of dislodged titanium particles in aseptic loosening following joint replacement surgery. This study predominantly investigates the suppressive influence of Kaempferol on osteolysis induced by titanium (Ti) alloy particles. In vitro investigations disclosed that Kaempferol effectively enhanced mineralization and alkaline phosphatase (ALP) activity in bone-marrow mesenchymal stem cells exposed to Ti particles. In addition, we conducted a comprehensive analysis of osteogenic differentiation microarray data_sets (GSE37676, GSE79814, and GSE114474) to identify differentially expressed genes. Significantly, Kaempferol upregulated the expression of critical osteogenic markers, including Runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osterix/Sp-7, and β-catenin. In vivo experiments, including H&E staining and Immunohistochemistry, provided compelling evidence that Kaempferol exerted a robust inhibitory effect on periprosthetic osteolysis in mice, with particularly pronounced results at higher doses. Moreover, it elevated the expression levels of osteogenic factors and Wnt/β-catenin signaling components. These findings collectively indicate that Kaempferol mitigates the hindrance to osteogenesis posed by titanium particles by activating the Runx2 and Wnt/β-catenin signaling pathways. This research lays a solid foundation for the prospective utilization of Kaempferol in the management of aseptic loosening following arthroplasty, offering promising therapeutic potential. |
| first_indexed | 2024-03-11T11:06:45Z |
| format | Article |
| id | doaj.art-99960a1b2036418d830bb05dc7da6bb0 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-03-11T11:06:45Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-99960a1b2036418d830bb05dc7da6bb02023-11-12T12:10:59ZengBMCEuropean Journal of Medical Research2047-783X2023-11-0128111510.1186/s40001-023-01469-wKaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivoWenkui Qiu0Zhenghui Li1Zhenyan Su2Lichao Cao3Lei Li4Xi Chen5Wanhong Zhang6Yanqing Li7Department of Orthopedics, Kaifeng Central HospitalDepartment of Neurosurgery, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou UniversityDepartment of Orthopedics, Kaifeng Central HospitalDepartment of Orthopedics, Kaifeng Central HospitalDepartment of Orthopedics, Kaifeng Central HospitalDepartment of Internal Medicine 3-Rheumatology and Immunology, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Universitätsklinikum ErlangenDepartment of Neurosurgery, Kaifeng Central HospitalDepartment of Orthopedics, Kaifeng Central HospitalAbstract Kaempferol has demonstrated notable positive effects on the osteogenic differentiation of mesenchymal stem cells (MSC) and osteoblasts. A substantial body of research has emphasized the role of dislodged titanium particles in aseptic loosening following joint replacement surgery. This study predominantly investigates the suppressive influence of Kaempferol on osteolysis induced by titanium (Ti) alloy particles. In vitro investigations disclosed that Kaempferol effectively enhanced mineralization and alkaline phosphatase (ALP) activity in bone-marrow mesenchymal stem cells exposed to Ti particles. In addition, we conducted a comprehensive analysis of osteogenic differentiation microarray data_sets (GSE37676, GSE79814, and GSE114474) to identify differentially expressed genes. Significantly, Kaempferol upregulated the expression of critical osteogenic markers, including Runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osterix/Sp-7, and β-catenin. In vivo experiments, including H&E staining and Immunohistochemistry, provided compelling evidence that Kaempferol exerted a robust inhibitory effect on periprosthetic osteolysis in mice, with particularly pronounced results at higher doses. Moreover, it elevated the expression levels of osteogenic factors and Wnt/β-catenin signaling components. These findings collectively indicate that Kaempferol mitigates the hindrance to osteogenesis posed by titanium particles by activating the Runx2 and Wnt/β-catenin signaling pathways. This research lays a solid foundation for the prospective utilization of Kaempferol in the management of aseptic loosening following arthroplasty, offering promising therapeutic potential.https://doi.org/10.1186/s40001-023-01469-w |
| spellingShingle | Wenkui Qiu Zhenghui Li Zhenyan Su Lichao Cao Lei Li Xi Chen Wanhong Zhang Yanqing Li Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo European Journal of Medical Research |
| title | Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo |
| title_full | Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo |
| title_fullStr | Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo |
| title_full_unstemmed | Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo |
| title_short | Kaempferol prevents aseptic loosening via enhance the Wnt/β-catenin signaling pathway in vitro and in vivo |
| title_sort | kaempferol prevents aseptic loosening via enhance the wnt β catenin signaling pathway in vitro and in vivo |
| url | https://doi.org/10.1186/s40001-023-01469-w |
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