Optimization and validation of cryostat temperature conditions for trans-reflectance mode FTIR microspectroscopic imaging of biological tissues

Optimization and validation of cryostat temperature conditions for trans-reflectance mode FTIR microspectroscopic imaging of biological tissues

In Fourier transform infrared (FTIR) microspectrocopy, the tissue preparation method is crucial, especially how the tissue is cryo-sectioned prior to the imaging requires special consideration. Having a temperature difference between the cutting blade and the specimen holder of the cryostat greatly...

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Bibliographic Details
Main Authors: Sumedha Liyanage, Rohan S. Dassanayake, Amal Bouyanfif, Erandathi Rajakaruna, Latha Ramalingam, Naima Moustaid-Moussa, Noureddine Abidi
Format: Article
Language:English
Published: Elsevier 2017-01-01
Series:MethodsX
Subjects:
Cryo-sectioning and FTIR microspectroscopic imaging of biological tissues
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016117300067

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