In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials
The antibacterial biofunctionality of bone implants is essential for the prevention and treatment of implant-associated infections (IAI). In vitro co-culture models are utilized to assess this and study bacteria-host cell interactions at the implant interface, aiding our understanding of biomaterial...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332771/full |
| _version_ | 1827357900683608064 |
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| author | Benedictus I. M. Eijkel Iulian Apachitei Lidy E. Fratila-Apachitei Amir A. Zadpoor |
| author_facet | Benedictus I. M. Eijkel Iulian Apachitei Lidy E. Fratila-Apachitei Amir A. Zadpoor |
| author_sort | Benedictus I. M. Eijkel |
| collection | DOAJ |
| description | The antibacterial biofunctionality of bone implants is essential for the prevention and treatment of implant-associated infections (IAI). In vitro co-culture models are utilized to assess this and study bacteria-host cell interactions at the implant interface, aiding our understanding of biomaterial and the immune response against IAI without impeding the peri-implant bone tissue regeneration. This paper reviews existing co-culture models together with their characteristics, results, and clinical relevance. A total of 36 studies were found involving in vitro co-culture models between bacteria and osteogenic or immune cells at the interface with orthopedic antibacterial biomaterials. Most studies (∼67%) involved co-culture models of osteogenic cells and bacteria (osteo-bac), while 33% were co-culture models of immune cells and bacterial cells (im-bac). All models involve direct co-culture of two different cell types. The cell seeding sequence (simultaneous, bacteria-first, and cell-first) was used to mimic clinically relevant conditions and showed the greatest effect on the outcome for both types of co-culture models. The im-bac models are considered more relevant for early peri-implant infections, whereas the osteo-bac models suit late infections. The limitations of the current models and future directions to develop more relevant co-culture models to address specific research questions are also discussed. |
| first_indexed | 2024-03-08T05:54:51Z |
| format | Article |
| id | doaj.art-999a68fa9acc4883af3dc722791d350f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T05:54:51Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-999a68fa9acc4883af3dc722791d350f2024-02-05T04:35:55ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-02-011210.3389/fbioe.2024.13327711332771In vitro co-culture models for the assessment of orthopedic antibacterial biomaterialsBenedictus I. M. EijkelIulian ApachiteiLidy E. Fratila-ApachiteiAmir A. ZadpoorThe antibacterial biofunctionality of bone implants is essential for the prevention and treatment of implant-associated infections (IAI). In vitro co-culture models are utilized to assess this and study bacteria-host cell interactions at the implant interface, aiding our understanding of biomaterial and the immune response against IAI without impeding the peri-implant bone tissue regeneration. This paper reviews existing co-culture models together with their characteristics, results, and clinical relevance. A total of 36 studies were found involving in vitro co-culture models between bacteria and osteogenic or immune cells at the interface with orthopedic antibacterial biomaterials. Most studies (∼67%) involved co-culture models of osteogenic cells and bacteria (osteo-bac), while 33% were co-culture models of immune cells and bacterial cells (im-bac). All models involve direct co-culture of two different cell types. The cell seeding sequence (simultaneous, bacteria-first, and cell-first) was used to mimic clinically relevant conditions and showed the greatest effect on the outcome for both types of co-culture models. The im-bac models are considered more relevant for early peri-implant infections, whereas the osteo-bac models suit late infections. The limitations of the current models and future directions to develop more relevant co-culture models to address specific research questions are also discussed.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332771/fullimplant-associated infectionsantibacterial biomaterialsin vitro co-culture modelsosteogenic cellsimmune cells |
| spellingShingle | Benedictus I. M. Eijkel Iulian Apachitei Lidy E. Fratila-Apachitei Amir A. Zadpoor In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials Frontiers in Bioengineering and Biotechnology implant-associated infections antibacterial biomaterials in vitro co-culture models osteogenic cells immune cells |
| title | In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials |
| title_full | In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials |
| title_fullStr | In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials |
| title_full_unstemmed | In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials |
| title_short | In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials |
| title_sort | in vitro co culture models for the assessment of orthopedic antibacterial biomaterials |
| topic | implant-associated infections antibacterial biomaterials in vitro co-culture models osteogenic cells immune cells |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332771/full |
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