Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows
Lipid-related metabolic disorders (LRMD) are prevalent in early lactation dairy cows, and have detrimental effects on productivity and health. Our objectives were to identify cows resistant or susceptible to LRMD using a ketosis induction protocol (KIP) to discover differentially expressed liver gen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-08-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/9/2558 |
| _version_ | 1797520541271195648 |
|---|---|
| author | Ryan S. Pralle Wenli Li Brianna N. Murphy Henry T. Holdorf Heather M. White |
| author_facet | Ryan S. Pralle Wenli Li Brianna N. Murphy Henry T. Holdorf Heather M. White |
| author_sort | Ryan S. Pralle |
| collection | DOAJ |
| description | Lipid-related metabolic disorders (LRMD) are prevalent in early lactation dairy cows, and have detrimental effects on productivity and health. Our objectives were to identify cows resistant or susceptible to LRMD using a ketosis induction protocol (KIP) to discover differentially expressed liver genes and metabolic pathways associated with disposition. Clustering cows based on postpartum lipid metabolite concentrations within dietary treatments identified cows more or less susceptible (MS vs. LS) to LRMD within the control treatment, and more or less resistant (MR vs. LR) within the KIP treatment. Whole-transcriptome RNA sequencing was performed on liver samples (−28, +1, and +14 days relative to calving) to assess differential gene and pathway expression (LS vs. MS; MR vs. LR; <i>n</i> = 3 cows per cluster). Cows within the MS and LR clusters had evidence of greater blood serum β-hydroxybutyrate concentration and liver triglyceride content than the LS and MR clusters, respectively. The inferred metabolism of differentially expressed genes suggested a role of immune response (i.e., interferon-inducible proteins and major histocompatibility complex molecules). Additionally, unique roles for glutathione metabolism and eicosanoid metabolism in modulating susceptibility and resistance, respectively, were implicated. Overall, this research provides novel insight into the role of immunometabolism in LRMD pathology, and suggests the potential for unique control points for LRMD progression and severity. |
| first_indexed | 2024-03-10T07:58:15Z |
| format | Article |
| id | doaj.art-999bc71cd3594e38b1e5cc376106312b |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T07:58:15Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-999bc71cd3594e38b1e5cc376106312b2023-11-22T11:41:58ZengMDPI AGAnimals2076-26152021-08-01119255810.3390/ani11092558Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein CowsRyan S. Pralle0Wenli Li1Brianna N. Murphy2Henry T. Holdorf3Heather M. White4Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USADairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USADairy Forage Research Center, USDA-Agricultural Research Service, Madison, WI 53706, USADepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USADepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USALipid-related metabolic disorders (LRMD) are prevalent in early lactation dairy cows, and have detrimental effects on productivity and health. Our objectives were to identify cows resistant or susceptible to LRMD using a ketosis induction protocol (KIP) to discover differentially expressed liver genes and metabolic pathways associated with disposition. Clustering cows based on postpartum lipid metabolite concentrations within dietary treatments identified cows more or less susceptible (MS vs. LS) to LRMD within the control treatment, and more or less resistant (MR vs. LR) within the KIP treatment. Whole-transcriptome RNA sequencing was performed on liver samples (−28, +1, and +14 days relative to calving) to assess differential gene and pathway expression (LS vs. MS; MR vs. LR; <i>n</i> = 3 cows per cluster). Cows within the MS and LR clusters had evidence of greater blood serum β-hydroxybutyrate concentration and liver triglyceride content than the LS and MR clusters, respectively. The inferred metabolism of differentially expressed genes suggested a role of immune response (i.e., interferon-inducible proteins and major histocompatibility complex molecules). Additionally, unique roles for glutathione metabolism and eicosanoid metabolism in modulating susceptibility and resistance, respectively, were implicated. Overall, this research provides novel insight into the role of immunometabolism in LRMD pathology, and suggests the potential for unique control points for LRMD progression and severity.https://www.mdpi.com/2076-2615/11/9/2558dairy cowtransition periodketosisfatty liverRNA-Seqclustering |
| spellingShingle | Ryan S. Pralle Wenli Li Brianna N. Murphy Henry T. Holdorf Heather M. White Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows Animals dairy cow transition period ketosis fatty liver RNA-Seq clustering |
| title | Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows |
| title_full | Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows |
| title_fullStr | Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows |
| title_full_unstemmed | Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows |
| title_short | Novel Facets of the Liver Transcriptome Are Associated with the Susceptibility and Resistance to Lipid-Related Metabolic Disorders in Periparturient Holstein Cows |
| title_sort | novel facets of the liver transcriptome are associated with the susceptibility and resistance to lipid related metabolic disorders in periparturient holstein cows |
| topic | dairy cow transition period ketosis fatty liver RNA-Seq clustering |
| url | https://www.mdpi.com/2076-2615/11/9/2558 |
| work_keys_str_mv | AT ryanspralle novelfacetsofthelivertranscriptomeareassociatedwiththesusceptibilityandresistancetolipidrelatedmetabolicdisordersinperiparturientholsteincows AT wenlili novelfacetsofthelivertranscriptomeareassociatedwiththesusceptibilityandresistancetolipidrelatedmetabolicdisordersinperiparturientholsteincows AT briannanmurphy novelfacetsofthelivertranscriptomeareassociatedwiththesusceptibilityandresistancetolipidrelatedmetabolicdisordersinperiparturientholsteincows AT henrytholdorf novelfacetsofthelivertranscriptomeareassociatedwiththesusceptibilityandresistancetolipidrelatedmetabolicdisordersinperiparturientholsteincows AT heathermwhite novelfacetsofthelivertranscriptomeareassociatedwiththesusceptibilityandresistancetolipidrelatedmetabolicdisordersinperiparturientholsteincows |