Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.)
Abstract Background Bixa orellana L. is a plant native to Brazil but grows in other parts of the world including Bangladesh. This plant has been used for many years by indigenous people around the globe for a variety of applications. In Bangladesh, it is known as Doigota and the plant is underutiliz...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2020-06-01
|
| Series: | Bulletin of the National Research Centre |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s42269-020-00349-1 |
| _version_ | 1819229841730306048 |
|---|---|
| author | Sium Ahmed Bilkis Mehrin Moni Shawon Ahmed Donald James Gomes Abdullah Mohammad Shohael |
| author_facet | Sium Ahmed Bilkis Mehrin Moni Shawon Ahmed Donald James Gomes Abdullah Mohammad Shohael |
| author_sort | Sium Ahmed |
| collection | DOAJ |
| description | Abstract Background Bixa orellana L. is a plant native to Brazil but grows in other parts of the world including Bangladesh. This plant has been used for many years by indigenous people around the globe for a variety of applications. In Bangladesh, it is known as Doigota and the plant is underutilized and reported endangered. The present study aimed to perform comparative profiling where aqueous, ethanolic, and methanolic extracts of the seed, branch, and leaf of B. orellana plants were investigated for in vitro phytochemicals, antioxidant, and antibacterial activities. Results Phytochemical screening indicated the presence of nine different phytochemicals in varying degrees, which suggests the chemical diversity of this plant. Quantification of phenolics, flavonoids, and tannins was performed where leaf extracts contained significantly higher quantities of these phytochemicals than extracts from seed and branch. The highest phenolics (186.02 mg/g) were found in the methanolic extract of the leaf, whereas the highest flavonoids (61.01 mg/g) and tannins (36.74 mg/g) were found in aqueous extract of the leaf. Antioxidant activity has been evaluated by DPPH scavenging assay where the aqueous extract of leaf showed 93.62% scavenging activity at 800 μg/mL extract concentration. Although the extracts prevented the growth of both Gram-positive and Gram-negative bacteria, the antibacterial activity (zone of inhibition) of aqueous and methanolic extracts of the leaves were higher than those of the seed and branch extracts against Gram-negative (Escherichia coli) and Gram-positive (Bacillus subtilis) bacteria, respectively. Conclusions We found that B. orellana leaf extracts possess the highest antibacterial activities. The extracts had significant antioxidant and free radical-scavenging activities. The antioxidant and antibacterial activities could be attributed to high flavonoid and phenolic contents. These results suggest that the extracts of B. orellana possess bioactive compounds that could be used for different purposes such as source of new antibiotics or alternative natural preservatives in food matrices due to mainly their antioxidant and antibacterial activities. |
| first_indexed | 2024-12-23T11:19:35Z |
| format | Article |
| id | doaj.art-999c0752f9e747f69b43163e9940dda7 |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-23T11:19:35Z |
| publishDate | 2020-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-999c0752f9e747f69b43163e9940dda72022-12-21T17:49:07ZengSpringerOpenBulletin of the National Research Centre2522-83072020-06-0144111010.1186/s42269-020-00349-1Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.)Sium Ahmed0Bilkis Mehrin Moni1Shawon Ahmed2Donald James Gomes3Abdullah Mohammad Shohael4Cell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar UniversityCell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar UniversityCell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar UniversityDepartment of Microbiology, Faculty of Biological Sciences, University of DhakaCell Genetics and Plant Biotechnology Laboratory, Department of Biotechnology and Genetic Engineering, Jahangirnagar UniversityAbstract Background Bixa orellana L. is a plant native to Brazil but grows in other parts of the world including Bangladesh. This plant has been used for many years by indigenous people around the globe for a variety of applications. In Bangladesh, it is known as Doigota and the plant is underutilized and reported endangered. The present study aimed to perform comparative profiling where aqueous, ethanolic, and methanolic extracts of the seed, branch, and leaf of B. orellana plants were investigated for in vitro phytochemicals, antioxidant, and antibacterial activities. Results Phytochemical screening indicated the presence of nine different phytochemicals in varying degrees, which suggests the chemical diversity of this plant. Quantification of phenolics, flavonoids, and tannins was performed where leaf extracts contained significantly higher quantities of these phytochemicals than extracts from seed and branch. The highest phenolics (186.02 mg/g) were found in the methanolic extract of the leaf, whereas the highest flavonoids (61.01 mg/g) and tannins (36.74 mg/g) were found in aqueous extract of the leaf. Antioxidant activity has been evaluated by DPPH scavenging assay where the aqueous extract of leaf showed 93.62% scavenging activity at 800 μg/mL extract concentration. Although the extracts prevented the growth of both Gram-positive and Gram-negative bacteria, the antibacterial activity (zone of inhibition) of aqueous and methanolic extracts of the leaves were higher than those of the seed and branch extracts against Gram-negative (Escherichia coli) and Gram-positive (Bacillus subtilis) bacteria, respectively. Conclusions We found that B. orellana leaf extracts possess the highest antibacterial activities. The extracts had significant antioxidant and free radical-scavenging activities. The antioxidant and antibacterial activities could be attributed to high flavonoid and phenolic contents. These results suggest that the extracts of B. orellana possess bioactive compounds that could be used for different purposes such as source of new antibiotics or alternative natural preservatives in food matrices due to mainly their antioxidant and antibacterial activities.http://link.springer.com/article/10.1186/s42269-020-00349-1AntibioticsAntioxidantsBioactive compoundsDoigotaPhytochemicals |
| spellingShingle | Sium Ahmed Bilkis Mehrin Moni Shawon Ahmed Donald James Gomes Abdullah Mohammad Shohael Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.) Bulletin of the National Research Centre Antibiotics Antioxidants Bioactive compounds Doigota Phytochemicals |
| title | Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.) |
| title_full | Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.) |
| title_fullStr | Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.) |
| title_full_unstemmed | Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.) |
| title_short | Comparative phytochemical, antioxidant, and antibacterial study of different parts of Doigota plants (Bixa orellana L.) |
| title_sort | comparative phytochemical antioxidant and antibacterial study of different parts of doigota plants bixa orellana l |
| topic | Antibiotics Antioxidants Bioactive compounds Doigota Phytochemicals |
| url | http://link.springer.com/article/10.1186/s42269-020-00349-1 |
| work_keys_str_mv | AT siumahmed comparativephytochemicalantioxidantandantibacterialstudyofdifferentpartsofdoigotaplantsbixaorellanal AT bilkismehrinmoni comparativephytochemicalantioxidantandantibacterialstudyofdifferentpartsofdoigotaplantsbixaorellanal AT shawonahmed comparativephytochemicalantioxidantandantibacterialstudyofdifferentpartsofdoigotaplantsbixaorellanal AT donaldjamesgomes comparativephytochemicalantioxidantandantibacterialstudyofdifferentpartsofdoigotaplantsbixaorellanal AT abdullahmohammadshohael comparativephytochemicalantioxidantandantibacterialstudyofdifferentpartsofdoigotaplantsbixaorellanal |