AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs
The early detection of tumors improves chances of decreased morbidity and prolonged survival. Serum biomarkers are convenient to use and have several advantages over other approaches, such as accuracy and straightforward protocols. Reliable biomarkers from easily accessible sources are warranted for...
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| Format: | Article |
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MDPI AG
2020-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/15/4075 |
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| author | Ji-Eun Lee Woo-Jin Song Hunjoo Lee Byung-Gak Kim Taeho Kim Changsun Lee Bonghwan Jang Hwa-Young Youn Ul-Soo Choi Dong-Ha Bhang |
| author_facet | Ji-Eun Lee Woo-Jin Song Hunjoo Lee Byung-Gak Kim Taeho Kim Changsun Lee Bonghwan Jang Hwa-Young Youn Ul-Soo Choi Dong-Ha Bhang |
| author_sort | Ji-Eun Lee |
| collection | DOAJ |
| description | The early detection of tumors improves chances of decreased morbidity and prolonged survival. Serum biomarkers are convenient to use and have several advantages over other approaches, such as accuracy and straightforward protocols. Reliable biomarkers from easily accessible sources are warranted for the development of cost-effective assays for routine screening, particularly in veterinary medicine. Extracellular c-AMP-dependent protein kinase A (ECPKA) is a cytosolic leakage enzyme. The diagnostic accuracy of detecting autoantibodies against ECPKA was found to be higher than that of ECPKA activity from enzymatic assays, which use a complicated method. Here, we investigated the diagnostic significance of measuring serum ECPKA autoantibody levels using an in-house kit (AniScan cancer detection kit; Biattic, Anyang, Korea). We used sera from 550 dogs, including healthy dogs and those with malignant and benign tumors. Serum ECPKA and immunoglobulin G were determined using the AniScan cancer detection kit. ECPKA autoantibody levels were significantly higher (<i>p</i> < 0.01) in malignant tumors than in benign tumors, non-tumor diseases, and healthy controls. On the basis of sensitivity and specificity values, AniScan ECPKA is a rapid and easy-to-use assay that can be applied to screen malignant tumors from benign tumors or other diseases in dogs. |
| first_indexed | 2024-03-10T18:18:03Z |
| format | Article |
| id | doaj.art-999cf9cc9d5e4fee9434c94315bad71d |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T18:18:03Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-999cf9cc9d5e4fee9434c94315bad71d2023-11-20T07:32:40ZengMDPI AGSensors1424-82202020-07-012015407510.3390/s20154075AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in DogsJi-Eun Lee0Woo-Jin Song1Hunjoo Lee2Byung-Gak Kim3Taeho Kim4Changsun Lee5Bonghwan Jang6Hwa-Young Youn7Ul-Soo Choi8Dong-Ha Bhang9Department of Veterinary Clinical Pathology and Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju 54896, KoreaDepartment of Veterinary Internal Medicine and Research Institute of Veterinary Science, College of Veterinary Medicine, Jeju National University, Jeju 63243, KoreaBiattic Inc., Anyang 14059, KoreaBiattic Inc., Anyang 14059, KoreaAID Animal Hospital, Seoul 06032, KoreaAID Animal Hospital, Seoul 06032, KoreaGoodmorning Pet Hospital, Seongnam 13613, KoreaLaboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, KoreaDepartment of Veterinary Clinical Pathology and Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University, Jeonju 54896, KoreaDepartment of Molecular and Cellular Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, KoreaThe early detection of tumors improves chances of decreased morbidity and prolonged survival. Serum biomarkers are convenient to use and have several advantages over other approaches, such as accuracy and straightforward protocols. Reliable biomarkers from easily accessible sources are warranted for the development of cost-effective assays for routine screening, particularly in veterinary medicine. Extracellular c-AMP-dependent protein kinase A (ECPKA) is a cytosolic leakage enzyme. The diagnostic accuracy of detecting autoantibodies against ECPKA was found to be higher than that of ECPKA activity from enzymatic assays, which use a complicated method. Here, we investigated the diagnostic significance of measuring serum ECPKA autoantibody levels using an in-house kit (AniScan cancer detection kit; Biattic, Anyang, Korea). We used sera from 550 dogs, including healthy dogs and those with malignant and benign tumors. Serum ECPKA and immunoglobulin G were determined using the AniScan cancer detection kit. ECPKA autoantibody levels were significantly higher (<i>p</i> < 0.01) in malignant tumors than in benign tumors, non-tumor diseases, and healthy controls. On the basis of sensitivity and specificity values, AniScan ECPKA is a rapid and easy-to-use assay that can be applied to screen malignant tumors from benign tumors or other diseases in dogs.https://www.mdpi.com/1424-8220/20/15/4075extracellular c-AMP-dependent protein kinase Abiomarkerdogcancertumor |
| spellingShingle | Ji-Eun Lee Woo-Jin Song Hunjoo Lee Byung-Gak Kim Taeho Kim Changsun Lee Bonghwan Jang Hwa-Young Youn Ul-Soo Choi Dong-Ha Bhang AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs Sensors extracellular c-AMP-dependent protein kinase A biomarker dog cancer tumor |
| title | AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs |
| title_full | AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs |
| title_fullStr | AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs |
| title_full_unstemmed | AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs |
| title_short | AniScan Using Extracellular Cyclic AMP-Dependent Protein Kinase A as a Serum Biomarker Assay for the Diagnosis of Malignant Tumors in Dogs |
| title_sort | aniscan using extracellular cyclic amp dependent protein kinase a as a serum biomarker assay for the diagnosis of malignant tumors in dogs |
| topic | extracellular c-AMP-dependent protein kinase A biomarker dog cancer tumor |
| url | https://www.mdpi.com/1424-8220/20/15/4075 |
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