Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease
Vaccines are widely employed in aquaculture to prevent bacterial infections, but their use by the U.S. catfish industry is very limited. One of the main diseases affecting catfish aquaculture is columnaris disease, caused by the bacterial pathogen <i>Flavobacterium columnare</i>. In 2011...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/11/1370 |
| _version_ | 1797508267336794112 |
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| author | Wenlong Cai Covadonga R. Arias |
| author_facet | Wenlong Cai Covadonga R. Arias |
| author_sort | Wenlong Cai |
| collection | DOAJ |
| description | Vaccines are widely employed in aquaculture to prevent bacterial infections, but their use by the U.S. catfish industry is very limited. One of the main diseases affecting catfish aquaculture is columnaris disease, caused by the bacterial pathogen <i>Flavobacterium columnare</i>. In 2011, a modified-live vaccine against columnaris disease was developed by selecting mutants that were resistant to rifampin. The previous study has suggested that this vaccine is stable, safe, and effective, but the mechanisms that resulted in attenuation remained uncharacterized. To understand the molecular basis for attenuation, a comparative genomic analysis was conducted to identify specific point mutations. The PacBio RS long-read sequencing platform was used to obtain draft genomes of the mutant attenuated strain (Fc1723) and the parent virulent strain (FcB27). Sequence-based genome comparison identified 16 single nucleotide polymorphisms (SNP) unique to the mutant. Genes that contained mutations were involved in rifampin resistance, gliding motility, DNA transcription, toxin secretion, and extracellular protease synthesis. The results also found that the vaccine strain formed biofilm at a significantly lower rate than the parent strain. These observations suggested that the rifampin-resistant phenotype and the associated attenuation of the vaccine strain result from the altered activity of RNA polymerase (RpoB) and possible disrupted protein secretion systems. |
| first_indexed | 2024-03-10T05:00:21Z |
| format | Article |
| id | doaj.art-99a1b2ec02a1483b8c10d357809917e9 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T05:00:21Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-99a1b2ec02a1483b8c10d357809917e92023-11-23T01:53:57ZengMDPI AGVaccines2076-393X2021-11-01911137010.3390/vaccines9111370Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris DiseaseWenlong Cai0Covadonga R. Arias1Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong 999077, ChinaSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL 36832, USAVaccines are widely employed in aquaculture to prevent bacterial infections, but their use by the U.S. catfish industry is very limited. One of the main diseases affecting catfish aquaculture is columnaris disease, caused by the bacterial pathogen <i>Flavobacterium columnare</i>. In 2011, a modified-live vaccine against columnaris disease was developed by selecting mutants that were resistant to rifampin. The previous study has suggested that this vaccine is stable, safe, and effective, but the mechanisms that resulted in attenuation remained uncharacterized. To understand the molecular basis for attenuation, a comparative genomic analysis was conducted to identify specific point mutations. The PacBio RS long-read sequencing platform was used to obtain draft genomes of the mutant attenuated strain (Fc1723) and the parent virulent strain (FcB27). Sequence-based genome comparison identified 16 single nucleotide polymorphisms (SNP) unique to the mutant. Genes that contained mutations were involved in rifampin resistance, gliding motility, DNA transcription, toxin secretion, and extracellular protease synthesis. The results also found that the vaccine strain formed biofilm at a significantly lower rate than the parent strain. These observations suggested that the rifampin-resistant phenotype and the associated attenuation of the vaccine strain result from the altered activity of RNA polymerase (RpoB) and possible disrupted protein secretion systems.https://www.mdpi.com/2076-393X/9/11/1370<i>Flavobacterium columnare</i>columnaris diseaseattenuated vaccinerifampincatfish |
| spellingShingle | Wenlong Cai Covadonga R. Arias Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease Vaccines <i>Flavobacterium columnare</i> columnaris disease attenuated vaccine rifampin catfish |
| title | Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease |
| title_full | Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease |
| title_fullStr | Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease |
| title_full_unstemmed | Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease |
| title_short | Deciphering the Molecular Basis for Attenuation of <i>Flavobacterium columnare</i> Strain Fc1723 Used as Modified Live Vaccine against Columnaris Disease |
| title_sort | deciphering the molecular basis for attenuation of i flavobacterium columnare i strain fc1723 used as modified live vaccine against columnaris disease |
| topic | <i>Flavobacterium columnare</i> columnaris disease attenuated vaccine rifampin catfish |
| url | https://www.mdpi.com/2076-393X/9/11/1370 |
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