Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components
We aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chr...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/7/1284 |
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| author | Xiaoyuan Wang Shuangshuang Wang Shasha Huang Lihua Zhang Zhenzhen Ge Liping Sun Wei Zong |
| author_facet | Xiaoyuan Wang Shuangshuang Wang Shasha Huang Lihua Zhang Zhenzhen Ge Liping Sun Wei Zong |
| author_sort | Xiaoyuan Wang |
| collection | DOAJ |
| description | We aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and then quantified by UPLC-MS using authenticated standards. The results showed that the optimal purifying conditions were D101 resin with a dosage of 3 g, four hours adsorption, three hours desorption time, and 60% ethanol as the eluent, producing the highest purification rate of 51%. The purified distiller’s grain extract exhibited stronger antioxidant activity than the unpurified extracts, which was assessed using DPPH and ABTS methods (IC<sub>50</sub> DPPH = 34.03 and 16.21 μg/mL, respectively; IC<sub>50</sub> ABTS = 20.31 and 5.73 μg/mL, respectively). UPLC-MS results indicated that (−)-epicatechin is the major compound found in distiller’s grain extract which was quantified as 562.7 μg/g extract, followed by ferulic acid (518.2 μg/g), <i>p</i>-hydroxybenzoic acid (417.7 μg/g), caffeic acid (217.1 μg/g), syringic acid (158.0 μg/g) and quercetin (147.8 μg/g). Two compounds, vanillic acid (66.5 μg/g) and gallic acid (41.4 μg/g), were found in lower concentrations. The findings of this study suggest that purification of polyphenolic compounds from distiller’s grain by macroporous resins is feasible, providing a new and effective method for the secondary use of distiller’s grain resources. |
| first_indexed | 2024-12-10T12:43:30Z |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T12:43:30Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-99a7dd91676c42ffae85ada1b56ec1cb2022-12-22T01:48:28ZengMDPI AGMolecules1420-30492019-04-01247128410.3390/molecules24071284molecules24071284Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic ComponentsXiaoyuan Wang0Shuangshuang Wang1Shasha Huang2Lihua Zhang3Zhenzhen Ge4Liping Sun5Wei Zong6School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaSchool of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Henan, ChinaWe aimed to purify polyphenols from distiller’s grain extract using macroporous resins and to identify its polyphenolic components. The influence of operational parameters on purification efficiency was investigated. The polyphenolic composition was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) and then quantified by UPLC-MS using authenticated standards. The results showed that the optimal purifying conditions were D101 resin with a dosage of 3 g, four hours adsorption, three hours desorption time, and 60% ethanol as the eluent, producing the highest purification rate of 51%. The purified distiller’s grain extract exhibited stronger antioxidant activity than the unpurified extracts, which was assessed using DPPH and ABTS methods (IC<sub>50</sub> DPPH = 34.03 and 16.21 μg/mL, respectively; IC<sub>50</sub> ABTS = 20.31 and 5.73 μg/mL, respectively). UPLC-MS results indicated that (−)-epicatechin is the major compound found in distiller’s grain extract which was quantified as 562.7 μg/g extract, followed by ferulic acid (518.2 μg/g), <i>p</i>-hydroxybenzoic acid (417.7 μg/g), caffeic acid (217.1 μg/g), syringic acid (158.0 μg/g) and quercetin (147.8 μg/g). Two compounds, vanillic acid (66.5 μg/g) and gallic acid (41.4 μg/g), were found in lower concentrations. The findings of this study suggest that purification of polyphenolic compounds from distiller’s grain by macroporous resins is feasible, providing a new and effective method for the secondary use of distiller’s grain resources.https://www.mdpi.com/1420-3049/24/7/1284distiller’s grainspolyphenolsmacroporous resinpurification |
| spellingShingle | Xiaoyuan Wang Shuangshuang Wang Shasha Huang Lihua Zhang Zhenzhen Ge Liping Sun Wei Zong Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components Molecules distiller’s grains polyphenols macroporous resin purification |
| title | Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components |
| title_full | Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components |
| title_fullStr | Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components |
| title_full_unstemmed | Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components |
| title_short | Purification of Polyphenols from Distiller’s Grains by Macroporous Resin and Analysis of the Polyphenolic Components |
| title_sort | purification of polyphenols from distiller s grains by macroporous resin and analysis of the polyphenolic components |
| topic | distiller’s grains polyphenols macroporous resin purification |
| url | https://www.mdpi.com/1420-3049/24/7/1284 |
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