Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas

Amorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordat...

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Main Authors: J.D. Corrêa Junior, S. Allodi, G.M. Amado-Filho, M. Farina
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 2000-02-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009
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author J.D. Corrêa Junior
S. Allodi
G.M. Amado-Filho
M. Farina
author_facet J.D. Corrêa Junior
S. Allodi
G.M. Amado-Filho
M. Farina
author_sort J.D. Corrêa Junior
collection DOAJ
description Amorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordatus from regions contaminated with zinc, and the elemental composition of hepatopancreal phosphate granules. Organisms were collected from the contaminated areas of Sepetiba Bay (SB) and Guanabara Bay (GB), and from a non-contaminated area, Ribeira Bay (RB). The first two sites are located near the metropolitan region of Rio de Janeiro city, Brazil. Atomic absorption spectroscopy (AAS) showed a significant difference (P<0.05) for zinc concentration in the hepatopancreas from organisms collected at the contaminated sites GB (210 ± 20 µg/g dry weight) and SB (181 ± 16 µg/g dry weight) compared to the non-contaminated site RB (76 ± 14 µg/g dry weight). Phosphate granules isolated from hepatopancreatic tissue were studied by electron diffraction (ED), energy dispersive X-ray analysis (EDX) and electron spectroscopic imaging (ESI). ED of granules presented no diffraction spots, indicating that these structures are in an amorphous state, while EDX of granules isolated from a contaminated area contained P, Ca and Zn. Mg, Cl and Fe were also found in some of the spectra. ESI showed that O, P and Ca were colocalized in the mineralized layers of most granules observed. The correlation between the results obtained by AAS and those obtained by microanalytical techniques suggests that the hepatopancreatic granules of U. cordatus may be related to the phenomenon of heavy metal retention.
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spelling doaj.art-99a87f4aad3242dea768aeab2d2dce732022-12-21T22:51:58ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2000-02-0133221722110.1590/S0100-879X2000000200009Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreasJ.D. Corrêa JuniorS. AllodiG.M. Amado-FilhoM. FarinaAmorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordatus from regions contaminated with zinc, and the elemental composition of hepatopancreal phosphate granules. Organisms were collected from the contaminated areas of Sepetiba Bay (SB) and Guanabara Bay (GB), and from a non-contaminated area, Ribeira Bay (RB). The first two sites are located near the metropolitan region of Rio de Janeiro city, Brazil. Atomic absorption spectroscopy (AAS) showed a significant difference (P<0.05) for zinc concentration in the hepatopancreas from organisms collected at the contaminated sites GB (210 ± 20 µg/g dry weight) and SB (181 ± 16 µg/g dry weight) compared to the non-contaminated site RB (76 ± 14 µg/g dry weight). Phosphate granules isolated from hepatopancreatic tissue were studied by electron diffraction (ED), energy dispersive X-ray analysis (EDX) and electron spectroscopic imaging (ESI). ED of granules presented no diffraction spots, indicating that these structures are in an amorphous state, while EDX of granules isolated from a contaminated area contained P, Ca and Zn. Mg, Cl and Fe were also found in some of the spectra. ESI showed that O, P and Ca were colocalized in the mineralized layers of most granules observed. The correlation between the results obtained by AAS and those obtained by microanalytical techniques suggests that the hepatopancreatic granules of U. cordatus may be related to the phenomenon of heavy metal retention.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009phosphate granuleshepatopancreaszincenergy dispersive X-ray analysisatomic absorption spectroscopyelectron spectroscopic imaging
spellingShingle J.D. Corrêa Junior
S. Allodi
G.M. Amado-Filho
M. Farina
Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
Brazilian Journal of Medical and Biological Research
phosphate granules
hepatopancreas
zinc
energy dispersive X-ray analysis
atomic absorption spectroscopy
electron spectroscopic imaging
title Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
title_full Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
title_fullStr Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
title_full_unstemmed Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
title_short Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
title_sort zinc accumulation in phosphate granules of ucides cordatus hepatopancreas
topic phosphate granules
hepatopancreas
zinc
energy dispersive X-ray analysis
atomic absorption spectroscopy
electron spectroscopic imaging
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009
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AT sallodi zincaccumulationinphosphategranulesofucidescordatushepatopancreas
AT gmamadofilho zincaccumulationinphosphategranulesofucidescordatushepatopancreas
AT mfarina zincaccumulationinphosphategranulesofucidescordatushepatopancreas