Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas
Amorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordat...
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Associação Brasileira de Divulgação Científica
2000-02-01
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Series: | Brazilian Journal of Medical and Biological Research |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009 |
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author | J.D. Corrêa Junior S. Allodi G.M. Amado-Filho M. Farina |
author_facet | J.D. Corrêa Junior S. Allodi G.M. Amado-Filho M. Farina |
author_sort | J.D. Corrêa Junior |
collection | DOAJ |
description | Amorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordatus from regions contaminated with zinc, and the elemental composition of hepatopancreal phosphate granules. Organisms were collected from the contaminated areas of Sepetiba Bay (SB) and Guanabara Bay (GB), and from a non-contaminated area, Ribeira Bay (RB). The first two sites are located near the metropolitan region of Rio de Janeiro city, Brazil. Atomic absorption spectroscopy (AAS) showed a significant difference (P<0.05) for zinc concentration in the hepatopancreas from organisms collected at the contaminated sites GB (210 ± 20 µg/g dry weight) and SB (181 ± 16 µg/g dry weight) compared to the non-contaminated site RB (76 ± 14 µg/g dry weight). Phosphate granules isolated from hepatopancreatic tissue were studied by electron diffraction (ED), energy dispersive X-ray analysis (EDX) and electron spectroscopic imaging (ESI). ED of granules presented no diffraction spots, indicating that these structures are in an amorphous state, while EDX of granules isolated from a contaminated area contained P, Ca and Zn. Mg, Cl and Fe were also found in some of the spectra. ESI showed that O, P and Ca were colocalized in the mineralized layers of most granules observed. The correlation between the results obtained by AAS and those obtained by microanalytical techniques suggests that the hepatopancreatic granules of U. cordatus may be related to the phenomenon of heavy metal retention. |
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spelling | doaj.art-99a87f4aad3242dea768aeab2d2dce732022-12-21T22:51:58ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2000-02-0133221722110.1590/S0100-879X2000000200009Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreasJ.D. Corrêa JuniorS. AllodiG.M. Amado-FilhoM. FarinaAmorphous phosphate granules are present in vertebrate and invertebrate organisms. The functions attributed to these structures depend on their mineral contents and organic matrix composition. In the present study we have determined zinc concentrations in the hepatopancreas of the crab Ucides cordatus from regions contaminated with zinc, and the elemental composition of hepatopancreal phosphate granules. Organisms were collected from the contaminated areas of Sepetiba Bay (SB) and Guanabara Bay (GB), and from a non-contaminated area, Ribeira Bay (RB). The first two sites are located near the metropolitan region of Rio de Janeiro city, Brazil. Atomic absorption spectroscopy (AAS) showed a significant difference (P<0.05) for zinc concentration in the hepatopancreas from organisms collected at the contaminated sites GB (210 ± 20 µg/g dry weight) and SB (181 ± 16 µg/g dry weight) compared to the non-contaminated site RB (76 ± 14 µg/g dry weight). Phosphate granules isolated from hepatopancreatic tissue were studied by electron diffraction (ED), energy dispersive X-ray analysis (EDX) and electron spectroscopic imaging (ESI). ED of granules presented no diffraction spots, indicating that these structures are in an amorphous state, while EDX of granules isolated from a contaminated area contained P, Ca and Zn. Mg, Cl and Fe were also found in some of the spectra. ESI showed that O, P and Ca were colocalized in the mineralized layers of most granules observed. The correlation between the results obtained by AAS and those obtained by microanalytical techniques suggests that the hepatopancreatic granules of U. cordatus may be related to the phenomenon of heavy metal retention.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009phosphate granuleshepatopancreaszincenergy dispersive X-ray analysisatomic absorption spectroscopyelectron spectroscopic imaging |
spellingShingle | J.D. Corrêa Junior S. Allodi G.M. Amado-Filho M. Farina Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas Brazilian Journal of Medical and Biological Research phosphate granules hepatopancreas zinc energy dispersive X-ray analysis atomic absorption spectroscopy electron spectroscopic imaging |
title | Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas |
title_full | Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas |
title_fullStr | Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas |
title_full_unstemmed | Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas |
title_short | Zinc accumulation in phosphate granules of Ucides cordatus hepatopancreas |
title_sort | zinc accumulation in phosphate granules of ucides cordatus hepatopancreas |
topic | phosphate granules hepatopancreas zinc energy dispersive X-ray analysis atomic absorption spectroscopy electron spectroscopic imaging |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000200009 |
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