Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis
Mastitis is the most devastating economic disease in dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or during early lactation. <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>)a...
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MDPI AG
2022-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/12/3282 |
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| author | Preeti Yadav Awadh Bihari Yadav Preksha Gaur Vartika Mishra Zul-I Huma Neelesh Sharma Young-Ok Son |
| author_facet | Preeti Yadav Awadh Bihari Yadav Preksha Gaur Vartika Mishra Zul-I Huma Neelesh Sharma Young-Ok Son |
| author_sort | Preeti Yadav |
| collection | DOAJ |
| description | Mastitis is the most devastating economic disease in dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or during early lactation. <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>)are the main causative agents of mastitis in India. <i>S. aureus</i> can form microabscesses in the udder and develop a subclinical form of mastitis. This bacterial property hinders an effective cure during the lactation period. Antimicrobials used for treatments have a short half-life at the site of action because of frequent milking; thereforethey are unable to maintain the desired drug concentration for effective clearance of bacteria. We demonstrated the potential of ciprofloxacin-encapsulated nanocarriersthat can improve the availability of drugs and provide an effective means for mastitis treatment. These drug-loaded nanoparticles show low toxicity and slow clearance from the site of action. Antimicrobial activity against clinical strains of <i>E. coli</i> and <i>S. aureus</i> showed that the zone of inhibition depended on the dose (0.5 mg to 2 mg/mL nanoparticle solution from 11.6 to 14.5 mm and 15 to 18 mm). These nanoparticles showed good antimicrobial activity in broth culture and agar diffusion assay against bacteria. |
| first_indexed | 2024-03-09T17:17:27Z |
| format | Article |
| id | doaj.art-99a973f2b6a04bbebd2a828931b0bf2e |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:17:27Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-99a973f2b6a04bbebd2a828931b0bf2e2023-11-24T13:29:59ZengMDPI AGBiomedicines2227-90592022-12-011012328210.3390/biomedicines10123282Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine MastitisPreeti Yadav0Awadh Bihari Yadav1Preksha Gaur2Vartika Mishra3Zul-I Huma4Neelesh Sharma5Young-Ok Son6Centre of Biotechnology, University of Allahabad, Pryagraj 211002, IndiaCentre of Biotechnology, University of Allahabad, Pryagraj 211002, IndiaCentre of Biotechnology, University of Allahabad, Pryagraj 211002, IndiaCentre of Biotechnology, University of Allahabad, Pryagraj 211002, IndiaDivision of Veterinary Medicine, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, R.S. Pura, Jammu 181102, IndiaDivision of Veterinary Medicine, Faculty of Veterinary Sciences and Animal Husbandry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, R.S. Pura, Jammu 181102, IndiaDepartment of Animal Biotechnology, Faculty of Biotechnology, College of Applied Life Sciences, Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 690756, Republic of KoreaMastitis is the most devastating economic disease in dairy cattle. Mastitis in dairy cattle frequently occurs during the dry period or during early lactation. <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>)are the main causative agents of mastitis in India. <i>S. aureus</i> can form microabscesses in the udder and develop a subclinical form of mastitis. This bacterial property hinders an effective cure during the lactation period. Antimicrobials used for treatments have a short half-life at the site of action because of frequent milking; thereforethey are unable to maintain the desired drug concentration for effective clearance of bacteria. We demonstrated the potential of ciprofloxacin-encapsulated nanocarriersthat can improve the availability of drugs and provide an effective means for mastitis treatment. These drug-loaded nanoparticles show low toxicity and slow clearance from the site of action. Antimicrobial activity against clinical strains of <i>E. coli</i> and <i>S. aureus</i> showed that the zone of inhibition depended on the dose (0.5 mg to 2 mg/mL nanoparticle solution from 11.6 to 14.5 mm and 15 to 18 mm). These nanoparticles showed good antimicrobial activity in broth culture and agar diffusion assay against bacteria.https://www.mdpi.com/2227-9059/10/12/3282mastitisciprofloxacinnanoparticlesdrug delivery |
| spellingShingle | Preeti Yadav Awadh Bihari Yadav Preksha Gaur Vartika Mishra Zul-I Huma Neelesh Sharma Young-Ok Son Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis Biomedicines mastitis ciprofloxacin nanoparticles drug delivery |
| title | Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis |
| title_full | Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis |
| title_fullStr | Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis |
| title_full_unstemmed | Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis |
| title_short | Bioengineered Ciprofloxacin-Loaded Chitosan Nanoparticles for the Treatment of Bovine Mastitis |
| title_sort | bioengineered ciprofloxacin loaded chitosan nanoparticles for the treatment of bovine mastitis |
| topic | mastitis ciprofloxacin nanoparticles drug delivery |
| url | https://www.mdpi.com/2227-9059/10/12/3282 |
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