Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame
Abstract Background As an important oil crop, growth habit of sesame (Sesamum indicum L.) is naturally indeterminate, which brings about asynchronous maturity of capsules and causes loss of yield. Results The genetic basis of determinate growth habit in sesame was investigated by classical genetic a...
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| Format: | Article |
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BMC
2018-06-01
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| Series: | BMC Genetics |
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| Online Access: | http://link.springer.com/article/10.1186/s12863-018-0614-y |
| _version_ | 1811291029754609664 |
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| author | Yanxin Zhang Linhai Wang Yuan Gao Donghua Li Jingyin Yu Rong Zhou Xiurong Zhang |
| author_facet | Yanxin Zhang Linhai Wang Yuan Gao Donghua Li Jingyin Yu Rong Zhou Xiurong Zhang |
| author_sort | Yanxin Zhang |
| collection | DOAJ |
| description | Abstract Background As an important oil crop, growth habit of sesame (Sesamum indicum L.) is naturally indeterminate, which brings about asynchronous maturity of capsules and causes loss of yield. Results The genetic basis of determinate growth habit in sesame was investigated by classical genetic analysis through multiple populations, results revealed that it was controlled by an unique recessive gene. The genotyping by sequencing (GBS) approach was employed for high-throughput SNP identification and genotyping in the F2 population, then a high density bin map was constructed, the map was 1086.403 cM in length, which consisted of 1184 bins (13,679 SNPs), with an average of 0.918 cM between adjacent bins. Based on bin mapping in conjunction with SSR markers analysis in targeted region, the novel sesame determinacy gene was mapped on LG09 in a genome region of 41 kb. Conclusions This study dissected genetic basis of determinate growth habit in sesame, constructed a new high-density bin map and mapped a novel determinacy gene. Results of this study demonstrate that we employed an optimized approach to get fine-accuracy, high-resolution and high-efficiency mapping result in sesame. The findings provided important foundation for sesame determinacy gene cloning and were expected to be applied in breeding for cultivars suited to mechanized production. |
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| format | Article |
| id | doaj.art-99aca9ca1bd1465da8db37a799ffb28e |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-13T04:23:13Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
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| series | BMC Genetics |
| spelling | doaj.art-99aca9ca1bd1465da8db37a799ffb28e2022-12-22T03:02:39ZengBMCBMC Genetics1471-21562018-06-0119111010.1186/s12863-018-0614-yGenetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesameYanxin Zhang0Linhai Wang1Yuan Gao2Donghua Li3Jingyin Yu4Rong Zhou5Xiurong Zhang6Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureOil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of AgricultureAbstract Background As an important oil crop, growth habit of sesame (Sesamum indicum L.) is naturally indeterminate, which brings about asynchronous maturity of capsules and causes loss of yield. Results The genetic basis of determinate growth habit in sesame was investigated by classical genetic analysis through multiple populations, results revealed that it was controlled by an unique recessive gene. The genotyping by sequencing (GBS) approach was employed for high-throughput SNP identification and genotyping in the F2 population, then a high density bin map was constructed, the map was 1086.403 cM in length, which consisted of 1184 bins (13,679 SNPs), with an average of 0.918 cM between adjacent bins. Based on bin mapping in conjunction with SSR markers analysis in targeted region, the novel sesame determinacy gene was mapped on LG09 in a genome region of 41 kb. Conclusions This study dissected genetic basis of determinate growth habit in sesame, constructed a new high-density bin map and mapped a novel determinacy gene. Results of this study demonstrate that we employed an optimized approach to get fine-accuracy, high-resolution and high-efficiency mapping result in sesame. The findings provided important foundation for sesame determinacy gene cloning and were expected to be applied in breeding for cultivars suited to mechanized production.http://link.springer.com/article/10.1186/s12863-018-0614-ySesamum indicum L.DeterminacyGenetic basisBin mapFine mapping |
| spellingShingle | Yanxin Zhang Linhai Wang Yuan Gao Donghua Li Jingyin Yu Rong Zhou Xiurong Zhang Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame BMC Genetics Sesamum indicum L. Determinacy Genetic basis Bin map Fine mapping |
| title | Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame |
| title_full | Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame |
| title_fullStr | Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame |
| title_full_unstemmed | Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame |
| title_short | Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame |
| title_sort | genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame |
| topic | Sesamum indicum L. Determinacy Genetic basis Bin map Fine mapping |
| url | http://link.springer.com/article/10.1186/s12863-018-0614-y |
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