The antiproliferative potential and mechanism of action of metformin in MCF-7 cells
Aim: The current study aimed to investigate the potential antiproliferative activity of metformin, the effective concentration range, and the mechanism of action. Materials & methods: Human breast cancer cells, MCF-7 were treated with a serial dilution of metformin (10–150 μM) for 24 and 48 h. P...
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| Format: | Article |
| Language: | English |
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Future Science Ltd
2023-06-01
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| Series: | Future Science OA |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/fsoa-2022-0080 |
| _version_ | 1797832918025895936 |
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| author | Zhibek Bekezhankyzy Sholpan Nurzhan Nurken Berdigaliyev Shynggys Sergazy Tilektes Maulenkul Mohamad Aljofan |
| author_facet | Zhibek Bekezhankyzy Sholpan Nurzhan Nurken Berdigaliyev Shynggys Sergazy Tilektes Maulenkul Mohamad Aljofan |
| author_sort | Zhibek Bekezhankyzy |
| collection | DOAJ |
| description | Aim: The current study aimed to investigate the potential antiproliferative activity of metformin, the effective concentration range, and the mechanism of action. Materials & methods: Human breast cancer cells, MCF-7 were treated with a serial dilution of metformin (10–150 μM) for 24 and 48 h. Potential antiproliferative activity of metformin and its ability in inducing cellular apoptosis and autophagy were also investigated. Results: Metformin inhibited MCF-7 proliferation in a concentration and time dependent manner, with 80 μM as the most effective concentration. Compared with nontreated cells, metformin induced significant levels of autophagy and apoptosis, which were confirmed by the reduction of mTOR and BCL-2 protein expression. Conclusion: The study confirms the antiproliferative activity of metformin, which may likely occur through AMPK signaling pathway. |
| first_indexed | 2024-04-09T14:15:26Z |
| format | Article |
| id | doaj.art-99b23fe8c66a44f4b57c9833a38ba7b2 |
| institution | Directory Open Access Journal |
| issn | 2056-5623 |
| language | English |
| last_indexed | 2024-04-09T14:15:26Z |
| publishDate | 2023-06-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj.art-99b23fe8c66a44f4b57c9833a38ba7b22023-05-05T11:17:58ZengFuture Science LtdFuture Science OA2056-56232023-06-019510.2144/fsoa-2022-0080The antiproliferative potential and mechanism of action of metformin in MCF-7 cellsZhibek Bekezhankyzy0Sholpan Nurzhan1Nurken Berdigaliyev2Shynggys Sergazy3Tilektes Maulenkul4Mohamad Aljofan51Department of Biomedical Sciences, School of Medicine, Nazarbayaev University, Astana, 010000, Kazakhstan1Department of Biomedical Sciences, School of Medicine, Nazarbayaev University, Astana, 010000, Kazakhstan1Department of Biomedical Sciences, School of Medicine, Nazarbayaev University, Astana, 010000, Kazakhstan2National Laboratory Astana, Nazarbayev University, Astana, 010000, Kazakhstan3Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, 161211, Turkistan, Kazakhstan1Department of Biomedical Sciences, School of Medicine, Nazarbayaev University, Astana, 010000, KazakhstanAim: The current study aimed to investigate the potential antiproliferative activity of metformin, the effective concentration range, and the mechanism of action. Materials & methods: Human breast cancer cells, MCF-7 were treated with a serial dilution of metformin (10–150 μM) for 24 and 48 h. Potential antiproliferative activity of metformin and its ability in inducing cellular apoptosis and autophagy were also investigated. Results: Metformin inhibited MCF-7 proliferation in a concentration and time dependent manner, with 80 μM as the most effective concentration. Compared with nontreated cells, metformin induced significant levels of autophagy and apoptosis, which were confirmed by the reduction of mTOR and BCL-2 protein expression. Conclusion: The study confirms the antiproliferative activity of metformin, which may likely occur through AMPK signaling pathway.https://www.future-science.com/doi/10.2144/fsoa-2022-0080antiproliferativeapoptosisautophagybreast cancermetformin |
| spellingShingle | Zhibek Bekezhankyzy Sholpan Nurzhan Nurken Berdigaliyev Shynggys Sergazy Tilektes Maulenkul Mohamad Aljofan The antiproliferative potential and mechanism of action of metformin in MCF-7 cells Future Science OA antiproliferative apoptosis autophagy breast cancer metformin |
| title | The antiproliferative potential and mechanism of action of metformin in MCF-7 cells |
| title_full | The antiproliferative potential and mechanism of action of metformin in MCF-7 cells |
| title_fullStr | The antiproliferative potential and mechanism of action of metformin in MCF-7 cells |
| title_full_unstemmed | The antiproliferative potential and mechanism of action of metformin in MCF-7 cells |
| title_short | The antiproliferative potential and mechanism of action of metformin in MCF-7 cells |
| title_sort | antiproliferative potential and mechanism of action of metformin in mcf 7 cells |
| topic | antiproliferative apoptosis autophagy breast cancer metformin |
| url | https://www.future-science.com/doi/10.2144/fsoa-2022-0080 |
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