The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration
Hengyu Tian, Yuanyuan Chen, Renjie Zhang, Jialin Liu Department of Hepatobiliary Surgery, Shenzhen Traditional Chinese Medicine Hospital/The fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, People’s Republic of ChinaCorrespondence: Jialin Liu,...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-02-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/the-role-of-col22a1-in-the-pathophysiology-of-hepatocellular-carcinoma-peer-reviewed-fulltext-article-CMAR |
| _version_ | 1818872347322482688 |
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| author | Tian H Chen Y Zhang R Liu J |
| author_facet | Tian H Chen Y Zhang R Liu J |
| author_sort | Tian H |
| collection | DOAJ |
| description | Hengyu Tian, Yuanyuan Chen, Renjie Zhang, Jialin Liu Department of Hepatobiliary Surgery, Shenzhen Traditional Chinese Medicine Hospital/The fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, People’s Republic of ChinaCorrespondence: Jialin Liu, Tel +86-13714754393, Email szliujialin123@163.comIntroduction: This work carried out comprehensive bioinformatics analysis and explored potential actions of COL22A1 in hepatocellular carcinoma (HCC) pathophysiology.Methods: Microarray datasets including GSE159220, GSE104766, GSE104310 and GSE33294 were retrieved for bioinformatics analysis. Overlapped differentially expressed genes (DEGs) among GSE159220, GSE104766, GSE104310 and GSE33294 were first subjected to functional annotation analysis. Protein–protein interaction (PPI) network was constructed to reveal the hub genes in the network. The miRNAs-mRNA from hub genes were also constructed. Prognostic analysis of patients with HCC was undertaken to evaluate prognostic values of these hub genes. Functional assays were employed to determine actions of COL22A1 in the HCC cell progression.Results: Ninety-one common up-regulated DEGs and 55 common down-regulated DEGs among GSE159220, GSE104766, GSE104310 and GSE33294 were identified. Functional annotation analysis revealed that these common DEGs may be correlated with the extracellular matrix. The protein–protein interaction analysis further revealed 20 hub genes. Among these hub genes, high expression levels of COL2A1, COL22A1, HAPLN1, PTHLH and SOX2 showed positive associatin with shorter overall survival (OS) of HCC patients, while low expression levels of COL17A1, REN and TRH exhibited correlation with shorter OS of HCC patients. Furthermore, the COL22A1 was overexpressed in HCC tissues when compared to normal ones, and the promoter methylation level of COL22A1 was significantly lower in the HCC tissues when compared to normal ones. Validation assays deciphered that COL22A1 silencing suppressed HCC cell invasion, migration and epithelial mesenchymal transition.Conclusion: This study identified several hub genes closely correlated with HCC pathogenesis, and COL22A1 may be involved in the HCC metastasis, but this still requires further mechanistic studies.Keywords: hepatocellular carcinoma, differentially expressed genes, hub genes, COL22A1, prognosis |
| first_indexed | 2024-12-19T12:37:22Z |
| format | Article |
| id | doaj.art-99b3e96ee41c420abd2ecc40aafd86bc |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-19T12:37:22Z |
| publishDate | 2022-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-99b3e96ee41c420abd2ecc40aafd86bc2022-12-21T20:21:06ZengDove Medical PressCancer Management and Research1179-13222022-02-01Volume 1473975073127The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics ExplorationTian HChen YZhang RLiu JHengyu Tian, Yuanyuan Chen, Renjie Zhang, Jialin Liu Department of Hepatobiliary Surgery, Shenzhen Traditional Chinese Medicine Hospital/The fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong, 518033, People’s Republic of ChinaCorrespondence: Jialin Liu, Tel +86-13714754393, Email szliujialin123@163.comIntroduction: This work carried out comprehensive bioinformatics analysis and explored potential actions of COL22A1 in hepatocellular carcinoma (HCC) pathophysiology.Methods: Microarray datasets including GSE159220, GSE104766, GSE104310 and GSE33294 were retrieved for bioinformatics analysis. Overlapped differentially expressed genes (DEGs) among GSE159220, GSE104766, GSE104310 and GSE33294 were first subjected to functional annotation analysis. Protein–protein interaction (PPI) network was constructed to reveal the hub genes in the network. The miRNAs-mRNA from hub genes were also constructed. Prognostic analysis of patients with HCC was undertaken to evaluate prognostic values of these hub genes. Functional assays were employed to determine actions of COL22A1 in the HCC cell progression.Results: Ninety-one common up-regulated DEGs and 55 common down-regulated DEGs among GSE159220, GSE104766, GSE104310 and GSE33294 were identified. Functional annotation analysis revealed that these common DEGs may be correlated with the extracellular matrix. The protein–protein interaction analysis further revealed 20 hub genes. Among these hub genes, high expression levels of COL2A1, COL22A1, HAPLN1, PTHLH and SOX2 showed positive associatin with shorter overall survival (OS) of HCC patients, while low expression levels of COL17A1, REN and TRH exhibited correlation with shorter OS of HCC patients. Furthermore, the COL22A1 was overexpressed in HCC tissues when compared to normal ones, and the promoter methylation level of COL22A1 was significantly lower in the HCC tissues when compared to normal ones. Validation assays deciphered that COL22A1 silencing suppressed HCC cell invasion, migration and epithelial mesenchymal transition.Conclusion: This study identified several hub genes closely correlated with HCC pathogenesis, and COL22A1 may be involved in the HCC metastasis, but this still requires further mechanistic studies.Keywords: hepatocellular carcinoma, differentially expressed genes, hub genes, COL22A1, prognosishttps://www.dovepress.com/the-role-of-col22a1-in-the-pathophysiology-of-hepatocellular-carcinoma-peer-reviewed-fulltext-article-CMARhepatocellular carcinomadifferentially expressed geneshub genescol22a1prognosis |
| spellingShingle | Tian H Chen Y Zhang R Liu J The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration Cancer Management and Research hepatocellular carcinoma differentially expressed genes hub genes col22a1 prognosis |
| title | The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration |
| title_full | The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration |
| title_fullStr | The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration |
| title_full_unstemmed | The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration |
| title_short | The Role of COL22A1 in the Pathophysiology of Hepatocellular Carcinoma: Evidence from Bioinformatics Exploration |
| title_sort | role of col22a1 in the pathophysiology of hepatocellular carcinoma evidence from bioinformatics exploration |
| topic | hepatocellular carcinoma differentially expressed genes hub genes col22a1 prognosis |
| url | https://www.dovepress.com/the-role-of-col22a1-in-the-pathophysiology-of-hepatocellular-carcinoma-peer-reviewed-fulltext-article-CMAR |
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