Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes
Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carb...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2019-05-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-12692-en.html |
| _version_ | 1797943869118087168 |
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| author | Masoumeh Beig Mohammad Reza Arabestani |
| author_facet | Masoumeh Beig Mohammad Reza Arabestani |
| author_sort | Masoumeh Beig |
| collection | DOAJ |
| description | Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes.
Materials and methods: A total of 97 clinical specimens was collected from patients in educational hospitals between November 2017 and May 2018 in Hamadan, Iran. Antibiotic susceptibility test was performed by disc diffusion method and MIC for imipenem was done by E-test. In order to detect carbapenemases enzymes, combined disk (CDT), CarbaNP, Modified Hodge (MHT), and Polymerase chain reaction (PCR) were performed. Statistical analysis was performed using SPSS V16.
Results: The highest and lowest levels of resistance were found to be to Ceftriaxone 65 (67%) and Piperacilin/Tazobactam 38 (39.2%), respectively. Among 97 clinical isolates of P.aeruginosa,
49 (50.51%) were positive for carbapenemase genes by PCR test from which 48 (97.95%) were positive for CarbaNP test. There were 48 (49.48%) PCR negative isolates of which all (100%) showed negative results for CarbaNP test. Compared to PCR, the sensitivity and specificity of this test was 98% and 100%, respectively.
Conclusion: This study showed that a high percentage of P. aeruginosa was resistant to Carbapenem antibiotics and the CarbaNP method was highly sensitive and specific for detection of carbapenemase enzymes. |
| first_indexed | 2024-04-10T20:30:56Z |
| format | Article |
| id | doaj.art-99b4eae5f80e4c2c859fceb40bd32a9c |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T20:30:56Z |
| publishDate | 2019-05-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-99b4eae5f80e4c2c859fceb40bd32a9c2023-01-25T06:57:44ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792019-05-01291721021Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase EnzymesMasoumeh Beig0Mohammad Reza Arabestani1 MSc in Medical Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran Associate professor, Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran 3 Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes. Materials and methods: A total of 97 clinical specimens was collected from patients in educational hospitals between November 2017 and May 2018 in Hamadan, Iran. Antibiotic susceptibility test was performed by disc diffusion method and MIC for imipenem was done by E-test. In order to detect carbapenemases enzymes, combined disk (CDT), CarbaNP, Modified Hodge (MHT), and Polymerase chain reaction (PCR) were performed. Statistical analysis was performed using SPSS V16. Results: The highest and lowest levels of resistance were found to be to Ceftriaxone 65 (67%) and Piperacilin/Tazobactam 38 (39.2%), respectively. Among 97 clinical isolates of P.aeruginosa, 49 (50.51%) were positive for carbapenemase genes by PCR test from which 48 (97.95%) were positive for CarbaNP test. There were 48 (49.48%) PCR negative isolates of which all (100%) showed negative results for CarbaNP test. Compared to PCR, the sensitivity and specificity of this test was 98% and 100%, respectively. Conclusion: This study showed that a high percentage of P. aeruginosa was resistant to Carbapenem antibiotics and the CarbaNP method was highly sensitive and specific for detection of carbapenemase enzymes.http://jmums.mazums.ac.ir/article-1-12692-en.htmlpseudomonas aeruginosadisk diffusion antibiotic testpcrcarbanp |
| spellingShingle | Masoumeh Beig Mohammad Reza Arabestani Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes Journal of Mazandaran University of Medical Sciences pseudomonas aeruginosa disk diffusion antibiotic test pcr carbanp |
| title | Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing
Carbapenemase Enzymes |
| title_full | Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing
Carbapenemase Enzymes |
| title_fullStr | Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing
Carbapenemase Enzymes |
| title_full_unstemmed | Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing
Carbapenemase Enzymes |
| title_short | Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing
Carbapenemase Enzymes |
| title_sort | evaluation of carba np method for rapid detection of pseudomonas aeruginosa isolates producing carbapenemase enzymes |
| topic | pseudomonas aeruginosa disk diffusion antibiotic test pcr carbanp |
| url | http://jmums.mazums.ac.ir/article-1-12692-en.html |
| work_keys_str_mv | AT masoumehbeig evaluationofcarbanpmethodforrapiddetectionofpseudomonasaeruginosaisolatesproducingcarbapenemaseenzymes AT mohammadrezaarabestani evaluationofcarbanpmethodforrapiddetectionofpseudomonasaeruginosaisolatesproducingcarbapenemaseenzymes |