Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy
Here, a formulation of silver nanoparticles (AgNPs) and two natural polymers such as alginate (ALG) and nanocrystalline cellulose (CNC) was developed for the 3D printing of scaffolds with large surface area, improved mechanical resistance and sustained capabilities to promote antimicrobial and cytot...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/5/844 |
| _version_ | 1827717883280490496 |
|---|---|
| author | Carlo Bergonzi Giulia Remaggi Claudia Graiff Laura Bergamonti Marianna Potenza Maria Cristina Ossiprandi Ilaria Zanotti Franco Bernini Ruggero Bettini Lisa Elviri |
| author_facet | Carlo Bergonzi Giulia Remaggi Claudia Graiff Laura Bergamonti Marianna Potenza Maria Cristina Ossiprandi Ilaria Zanotti Franco Bernini Ruggero Bettini Lisa Elviri |
| author_sort | Carlo Bergonzi |
| collection | DOAJ |
| description | Here, a formulation of silver nanoparticles (AgNPs) and two natural polymers such as alginate (ALG) and nanocrystalline cellulose (CNC) was developed for the 3D printing of scaffolds with large surface area, improved mechanical resistance and sustained capabilities to promote antimicrobial and cytotoxic effects. Mechanical resistance, water content, morphological characterization and silver distribution of the scaffolds were provided. As for applications, a comparable antimicrobial potency against <i>S. aureus</i> and <i>P. aeruginosa</i> was demonstrated by in vitro tests as function of the AgNP concentration in the scaffold (Minimal Inhibitory Concentration value: 10 mg/mL). By reusing the 3D system the antimicrobial efficacy was demonstrated over at least three applications. The cytotoxicity effects caused by administration of AgNPs to hepatocellular carcinoma (HepG2) cell culture through ALG and ALG/CNC scaffold were discussed as a function of time and dose. Finally, the liquid chromatography-mass spectrometry (LC-MS) technique was used for targeted analysis of pro-apoptotic initiation and executioner caspases, anti-apoptotic and proliferative proteins and the hepatocyte growth factor, and provided insights about molecular mechanisms involved in cell death induction. |
| first_indexed | 2024-03-10T20:11:37Z |
| format | Article |
| id | doaj.art-99ba64c730004ff7b9841adc3e695b8e |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T20:11:37Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-99ba64c730004ff7b9841adc3e695b8e2023-11-19T22:54:50ZengMDPI AGNanomaterials2079-49912020-04-0110584410.3390/nano10050844Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity EfficacyCarlo Bergonzi0Giulia Remaggi1Claudia Graiff2Laura Bergamonti3Marianna Potenza4Maria Cristina Ossiprandi5Ilaria Zanotti6Franco Bernini7Ruggero Bettini8Lisa Elviri9Food and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze 17/A, 43124 Parma, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyFood and Drug Department, University of Parma, Parco Area delle Scienze 27/a, 43124 Parma, ItalyHere, a formulation of silver nanoparticles (AgNPs) and two natural polymers such as alginate (ALG) and nanocrystalline cellulose (CNC) was developed for the 3D printing of scaffolds with large surface area, improved mechanical resistance and sustained capabilities to promote antimicrobial and cytotoxic effects. Mechanical resistance, water content, morphological characterization and silver distribution of the scaffolds were provided. As for applications, a comparable antimicrobial potency against <i>S. aureus</i> and <i>P. aeruginosa</i> was demonstrated by in vitro tests as function of the AgNP concentration in the scaffold (Minimal Inhibitory Concentration value: 10 mg/mL). By reusing the 3D system the antimicrobial efficacy was demonstrated over at least three applications. The cytotoxicity effects caused by administration of AgNPs to hepatocellular carcinoma (HepG2) cell culture through ALG and ALG/CNC scaffold were discussed as a function of time and dose. Finally, the liquid chromatography-mass spectrometry (LC-MS) technique was used for targeted analysis of pro-apoptotic initiation and executioner caspases, anti-apoptotic and proliferative proteins and the hepatocyte growth factor, and provided insights about molecular mechanisms involved in cell death induction.https://www.mdpi.com/2079-4991/10/5/8443D printingalginate/nanocrystalline cellulose/silver nanoparticles hydrogelsantimicrobial activityHepG2 cytotoxicity |
| spellingShingle | Carlo Bergonzi Giulia Remaggi Claudia Graiff Laura Bergamonti Marianna Potenza Maria Cristina Ossiprandi Ilaria Zanotti Franco Bernini Ruggero Bettini Lisa Elviri Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy Nanomaterials 3D printing alginate/nanocrystalline cellulose/silver nanoparticles hydrogels antimicrobial activity HepG2 cytotoxicity |
| title | Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy |
| title_full | Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy |
| title_fullStr | Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy |
| title_full_unstemmed | Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy |
| title_short | Three-Dimensional (3D) Printed Silver Nanoparticles/Alginate/Nanocrystalline Cellulose Hydrogels: Study of the Antimicrobial and Cytotoxicity Efficacy |
| title_sort | three dimensional 3d printed silver nanoparticles alginate nanocrystalline cellulose hydrogels study of the antimicrobial and cytotoxicity efficacy |
| topic | 3D printing alginate/nanocrystalline cellulose/silver nanoparticles hydrogels antimicrobial activity HepG2 cytotoxicity |
| url | https://www.mdpi.com/2079-4991/10/5/844 |
| work_keys_str_mv | AT carlobergonzi threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT giuliaremaggi threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT claudiagraiff threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT laurabergamonti threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT mariannapotenza threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT mariacristinaossiprandi threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT ilariazanotti threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT francobernini threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT ruggerobettini threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy AT lisaelviri threedimensional3dprintedsilvernanoparticlesalginatenanocrystallinecellulosehydrogelsstudyoftheantimicrobialandcytotoxicityefficacy |