In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples
Obtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and to develop new ex vivo strategies to generate an efficient anti-tumor response. The present study delineates a simple and rapid method for efficiently establis...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-03-01
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| Series: | Medical Sciences |
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| Online Access: | http://www.mdpi.com/2076-3271/2/2/70 |
| _version_ | 1828805667203317760 |
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| author | Ilaria Turin Roberta Schiavo Marcello Maestri Ombretta Luinetti Barbara Dal Bello Marco Paulli Paolo Dionigi Marianna Roccio Arsenio Spinillo Federica Ferulli Matteo Tanzi Rita Maccario Daniela Montagna Paolo Pedrazzoli |
| author_facet | Ilaria Turin Roberta Schiavo Marcello Maestri Ombretta Luinetti Barbara Dal Bello Marco Paulli Paolo Dionigi Marianna Roccio Arsenio Spinillo Federica Ferulli Matteo Tanzi Rita Maccario Daniela Montagna Paolo Pedrazzoli |
| author_sort | Ilaria Turin |
| collection | DOAJ |
| description | Obtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and to develop new ex vivo strategies to generate an efficient anti-tumor response. The present study delineates a simple and rapid method for efficiently establishing primary cultures starting from tumor samples of different types, while maintaining the immuno-histochemical characteristics of the original tumor. We compared two different strategies to disaggregate tumor specimens. After short or long term in vitro expansion, cells analyzed for the presence of malignant cells demonstrated their neoplastic origin. Considering that tumor cells may be isolated in a closed system with high efficiency, we propose this methodology for the ex vivo expansion of tumor cells to be used to evaluate suitable new drugs or to generate tumor-specific cytotoxic T lymphocytes or vaccines. |
| first_indexed | 2024-12-12T07:58:42Z |
| format | Article |
| id | doaj.art-99c0319848a8444fb626850f3c4b9410 |
| institution | Directory Open Access Journal |
| issn | 2076-3271 |
| language | English |
| last_indexed | 2024-12-12T07:58:42Z |
| publishDate | 2014-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medical Sciences |
| spelling | doaj.art-99c0319848a8444fb626850f3c4b94102022-12-22T00:32:13ZengMDPI AGMedical Sciences2076-32712014-03-0122708110.3390/medsci2020070medsci2020070In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor SamplesIlaria Turin0Roberta Schiavo1Marcello Maestri2Ombretta Luinetti3Barbara Dal Bello4Marco Paulli5Paolo Dionigi6Marianna Roccio7Arsenio Spinillo8Federica Ferulli9Matteo Tanzi10Rita Maccario11Daniela Montagna12Paolo Pedrazzoli13Laboratorio di Immunologia dei Trapianti, Oncoematologia Pediatrica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyU.O. Microbiologia, Ospedale Guglielmo Da Saliceto, Piacenza 29100, ItalyChirurgia Generale I Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyU.C. Anatomia Patologica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyU.C. Anatomia Patologica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyU.C. Anatomia Patologica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyChirurgia Generale I Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyS.C. Ostetricia e Ginecologia, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyDipartimento di Scienze Clinico-chirurgiche, Pediatriche e Diagnostiche, Università di Pavia, Pavia 20100, ItalyLaboratorio di Immunologia dei Trapianti, Oncoematologia Pediatrica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyLaboratorio di Immunologia dei Trapianti, Oncoematologia Pediatrica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyCell Factory, Lab. Ricerca, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyLaboratorio di Immunologia dei Trapianti, Oncoematologia Pediatrica, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyS.C. Oncologia, Fondazione IRCCS Policlinico S. Matteo, Pavia 27100, ItalyObtaining human tumor cell lines from fresh tumors is essential to advance our understanding of antitumor immune surveillance mechanisms and to develop new ex vivo strategies to generate an efficient anti-tumor response. The present study delineates a simple and rapid method for efficiently establishing primary cultures starting from tumor samples of different types, while maintaining the immuno-histochemical characteristics of the original tumor. We compared two different strategies to disaggregate tumor specimens. After short or long term in vitro expansion, cells analyzed for the presence of malignant cells demonstrated their neoplastic origin. Considering that tumor cells may be isolated in a closed system with high efficiency, we propose this methodology for the ex vivo expansion of tumor cells to be used to evaluate suitable new drugs or to generate tumor-specific cytotoxic T lymphocytes or vaccines.http://www.mdpi.com/2076-3271/2/2/70tumor cell linein vitro modelcell cultureimmunomagnetic selection |
| spellingShingle | Ilaria Turin Roberta Schiavo Marcello Maestri Ombretta Luinetti Barbara Dal Bello Marco Paulli Paolo Dionigi Marianna Roccio Arsenio Spinillo Federica Ferulli Matteo Tanzi Rita Maccario Daniela Montagna Paolo Pedrazzoli In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples Medical Sciences tumor cell line in vitro model cell culture immunomagnetic selection |
| title | In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples |
| title_full | In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples |
| title_fullStr | In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples |
| title_full_unstemmed | In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples |
| title_short | In Vitro Efficient Expansion of Tumor Cells Deriving from Different Types of Human Tumor Samples |
| title_sort | in vitro efficient expansion of tumor cells deriving from different types of human tumor samples |
| topic | tumor cell line in vitro model cell culture immunomagnetic selection |
| url | http://www.mdpi.com/2076-3271/2/2/70 |
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