Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus.

In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes based on the G or M gene of aMPV-A, aMPV-B, aMPV-...

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Main Authors: Suchun Wang, Nan Jiang, Lijian Jiang, Qingye Zhuang, Qiong Chen, Guangyu Hou, Zhiyu Xiao, Ran Zhao, Yang Li, Chenglong Zhao, Fuyou Zhang, Jianmin Yu, Jinping Li, Hualei Liu, Fuliang Sun, Kaicheng Wang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0270708

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