Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya
Abstract Background Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both s...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-05-01
|
| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-020-02346-y |
| _version_ | 1819056929902690304 |
|---|---|
| author | James Akoko Roger Pelle Velma Kivali Esther Schelling Gabriel Shirima Eunice M. Machuka Coletha Mathew Eric M. Fèvre Victoria Kyallo Laura C. Falzon AbdulHamid S. Lukambagire Jo E. B. Halliday Bassirou Bonfoh Rudovick Kazwala Collins Ouma |
| author_facet | James Akoko Roger Pelle Velma Kivali Esther Schelling Gabriel Shirima Eunice M. Machuka Coletha Mathew Eric M. Fèvre Victoria Kyallo Laura C. Falzon AbdulHamid S. Lukambagire Jo E. B. Halliday Bassirou Bonfoh Rudovick Kazwala Collins Ouma |
| author_sort | James Akoko |
| collection | DOAJ |
| description | Abstract Background Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs. |
| first_indexed | 2024-12-21T13:31:14Z |
| format | Article |
| id | doaj.art-99c4aba5f9df47a9b296f493b9c10c4e |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T13:31:14Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-99c4aba5f9df47a9b296f493b9c10c4e2022-12-21T19:02:17ZengBMCBMC Veterinary Research1746-61482020-05-011611710.1186/s12917-020-02346-ySerological and molecular evidence of Brucella species in the rapidly growing pig sector in KenyaJames Akoko0Roger Pelle1Velma Kivali2Esther Schelling3Gabriel Shirima4Eunice M. Machuka5Coletha Mathew6Eric M. Fèvre7Victoria Kyallo8Laura C. Falzon9AbdulHamid S. Lukambagire10Jo E. B. Halliday11Bassirou Bonfoh12Rudovick Kazwala13Collins Ouma14Department of Biomedical Sciences and Technology, Maseno UniversityBiosciences Eastern And Central Africa - International Livestock Research InstituteInternational Livestock Research InstituteDepartment of Public Health and Epidemiology, Swiss Tropical InstituteNelson Mandela African Institute of Science and TechnologyBiosciences Eastern And Central Africa - International Livestock Research InstituteSokoine University of AgricultureInternational Livestock Research InstituteInternational Livestock Research InstituteInternational Livestock Research InstituteSokoine University of AgricultureUniversity of GlasgowCentre Suisse de Recherches Scientifiques en Côte d’IvoireSokoine University of AgricultureDepartment of Biomedical Sciences and Technology, Maseno UniversityAbstract Background Brucellosis is an emerging yet neglected zoonosis that has been reported in Kenya. Epidemiological data on brucellosis in ruminants is readily accessible; however, reports on brucellosis in pigs remain limited. This study sought to detect Brucella infection in pig serum by both serological and molecular techniques. Serum from 700 pigs randomly collected at a centralized abattoir in Nairobi region, Kenya were screened in parallel, using both Rose Bengal Test (RBT) and competitive Enzyme-Linked Immuno-sorbent Assay (cELISA) for antibodies against Brucella spp. All sera positive by RBT and 16 randomly selected negative samples were further tested using conventional PCR targeting bcsp31 gene and real-time PCR (RT-PCR) assays targeting IS711 and bcsp31 genes. Results A prevalence of 0.57% (n = 4/700) was estimated using RBT; none of these samples was positive on cELISA. All RBT positive sera were also positive by both PCRs, while two sero-negative samples also tested positive on RT-PCR (n = 6/20). Brucella abortus was detected in four out of the six PCR positive samples through a real-time multiplex PCR. Conclusion The detection of antibodies against Brucella spp. and DNA in serum from slaughterhouse pigs confirm the presence of Brucella in pigs. Therefore, investigation of the epidemiology and role of pigs in the transmission of brucellosis in Kenya is needed. Further targeted studies would be useful to systematically quantify and identify the spp. of Brucella in pigs.http://link.springer.com/article/10.1186/s12917-020-02346-y“Pig brucellosis”“Molecular detection”“Molecular evidence”BrucellaSerologyKenya |
| spellingShingle | James Akoko Roger Pelle Velma Kivali Esther Schelling Gabriel Shirima Eunice M. Machuka Coletha Mathew Eric M. Fèvre Victoria Kyallo Laura C. Falzon AbdulHamid S. Lukambagire Jo E. B. Halliday Bassirou Bonfoh Rudovick Kazwala Collins Ouma Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya BMC Veterinary Research “Pig brucellosis” “Molecular detection” “Molecular evidence” Brucella Serology Kenya |
| title | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_full | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_fullStr | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_full_unstemmed | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_short | Serological and molecular evidence of Brucella species in the rapidly growing pig sector in Kenya |
| title_sort | serological and molecular evidence of brucella species in the rapidly growing pig sector in kenya |
| topic | “Pig brucellosis” “Molecular detection” “Molecular evidence” Brucella Serology Kenya |
| url | http://link.springer.com/article/10.1186/s12917-020-02346-y |
| work_keys_str_mv | AT jamesakoko serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT rogerpelle serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT velmakivali serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT estherschelling serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT gabrielshirima serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT eunicemmachuka serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT colethamathew serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT ericmfevre serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT victoriakyallo serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT lauracfalzon serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT abdulhamidslukambagire serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT joebhalliday serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT bassiroubonfoh serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT rudovickkazwala serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya AT collinsouma serologicalandmolecularevidenceofbrucellaspeciesintherapidlygrowingpigsectorinkenya |