Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs
Abstract Background Mesenchymal stromal cells (MSCs) have an inherent migratory capacity towards tumor tissue in vivo. With the future objective to quantify the tumor homing efficacy of MSCs, as first step in this direction we investigated the use of inorganic nanoparticles (NPs), in particular ca....
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-03-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-017-0258-5 |
| _version_ | 1811301457709760512 |
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| author | Philipp Nold Raimo Hartmann Neus Feliu Karsten Kantner Mahmoud Gamal Beatriz Pelaz Jonas Hühn Xing Sun Philipp Jungebluth Pablo del Pino Holger Hackstein Paolo Macchiarini Wolfgang J. Parak Cornelia Brendel |
| author_facet | Philipp Nold Raimo Hartmann Neus Feliu Karsten Kantner Mahmoud Gamal Beatriz Pelaz Jonas Hühn Xing Sun Philipp Jungebluth Pablo del Pino Holger Hackstein Paolo Macchiarini Wolfgang J. Parak Cornelia Brendel |
| author_sort | Philipp Nold |
| collection | DOAJ |
| description | Abstract Background Mesenchymal stromal cells (MSCs) have an inherent migratory capacity towards tumor tissue in vivo. With the future objective to quantify the tumor homing efficacy of MSCs, as first step in this direction we investigated the use of inorganic nanoparticles (NPs), in particular ca. 4 nm-sized Au NPs, for MSC labeling. Time dependent uptake efficiencies of NPs at different exposure concentrations and times were determined via inductively coupled plasma mass spectrometry (ICP-MS). Results The labeling efficiency of the MSCs was determined in terms of the amount of exocytosed NPs versus the amount of initially endocytosed NPs, demonstrating that at high concentrations the internalized Au NPs were exocytosed over time, leading to continuous exhaustion. While exposure to NPs did not significantly impair cell viability or expression of surface markers, even at high dose levels, MSCs were significantly affected in their proliferation and migration potential. These results demonstrate that proliferation or migration assays are more suitable to evaluate whether labeling of MSCs with certain amounts of NPs exerts distress on cells. However, despite optimized conditions the labeling efficiency varied considerably in MSC lots from different donors, indicating cell specific loading capacities for NPs. Finally, we determined the detection limits of Au NP-labeled MSCs within murine tissue employing ICP-MS and demonstrate the distribution and homing of NP labeled MSCs in vivo. Conclusion Although large amounts of NPs improve contrast for imaging, duration and extend of labeling needs to be adjusted carefully to avoid functional deficits in MSCs. We established an optimized labeling strategy for human MSCs with Au NPs that preserves their migratory capacity in vivo. |
| first_indexed | 2024-04-13T07:09:16Z |
| format | Article |
| id | doaj.art-99c96225f91644bba7aa3364dbe75a5b |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T07:09:16Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-99c96225f91644bba7aa3364dbe75a5b2022-12-22T02:56:55ZengBMCJournal of Nanobiotechnology1477-31552017-03-0115111110.1186/s12951-017-0258-5Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCsPhilipp Nold0Raimo Hartmann1Neus Feliu2Karsten Kantner3Mahmoud Gamal4Beatriz Pelaz5Jonas Hühn6Xing Sun7Philipp Jungebluth8Pablo del Pino9Holger Hackstein10Paolo Macchiarini11Wolfgang J. Parak12Cornelia Brendel13Department of Hematology, Oncology and Immunology, Philipps University MarburgDepartment of Physics, Philipps-University of MarburgDepartment of Physics, Philipps-University of MarburgDepartment of Physics, Philipps-University of MarburgDepartment of Physics, Philipps-University of MarburgDepartment of Physics, Philipps-University of MarburgDepartment of Physics, Philipps-University of MarburgDepartment of Physics, Philipps-University of MarburgThoraxklinik at Heidelberg University HospitalDepartment of Physics, Philipps-University of MarburgInstitute for Clinical Immunology and Transfusion Medicine, Justus-Liebig University GiessenLaboratory of Bioengineering & Regenerative Medicine (BioReM), Kazan Federal UniversityDepartment of Physics, Philipps-University of MarburgDepartment of Hematology, Oncology and Immunology, Philipps University MarburgAbstract Background Mesenchymal stromal cells (MSCs) have an inherent migratory capacity towards tumor tissue in vivo. With the future objective to quantify the tumor homing efficacy of MSCs, as first step in this direction we investigated the use of inorganic nanoparticles (NPs), in particular ca. 4 nm-sized Au NPs, for MSC labeling. Time dependent uptake efficiencies of NPs at different exposure concentrations and times were determined via inductively coupled plasma mass spectrometry (ICP-MS). Results The labeling efficiency of the MSCs was determined in terms of the amount of exocytosed NPs versus the amount of initially endocytosed NPs, demonstrating that at high concentrations the internalized Au NPs were exocytosed over time, leading to continuous exhaustion. While exposure to NPs did not significantly impair cell viability or expression of surface markers, even at high dose levels, MSCs were significantly affected in their proliferation and migration potential. These results demonstrate that proliferation or migration assays are more suitable to evaluate whether labeling of MSCs with certain amounts of NPs exerts distress on cells. However, despite optimized conditions the labeling efficiency varied considerably in MSC lots from different donors, indicating cell specific loading capacities for NPs. Finally, we determined the detection limits of Au NP-labeled MSCs within murine tissue employing ICP-MS and demonstrate the distribution and homing of NP labeled MSCs in vivo. Conclusion Although large amounts of NPs improve contrast for imaging, duration and extend of labeling needs to be adjusted carefully to avoid functional deficits in MSCs. We established an optimized labeling strategy for human MSCs with Au NPs that preserves their migratory capacity in vivo.http://link.springer.com/article/10.1186/s12951-017-0258-5Mesenchymal stromal cells (MSCs)Au nanoparticles (Au NP), labeling, in vivo tracking |
| spellingShingle | Philipp Nold Raimo Hartmann Neus Feliu Karsten Kantner Mahmoud Gamal Beatriz Pelaz Jonas Hühn Xing Sun Philipp Jungebluth Pablo del Pino Holger Hackstein Paolo Macchiarini Wolfgang J. Parak Cornelia Brendel Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs Journal of Nanobiotechnology Mesenchymal stromal cells (MSCs) Au nanoparticles (Au NP), labeling, in vivo tracking |
| title | Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs |
| title_full | Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs |
| title_fullStr | Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs |
| title_full_unstemmed | Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs |
| title_short | Optimizing conditions for labeling of mesenchymal stromal cells (MSCs) with gold nanoparticles: a prerequisite for in vivo tracking of MSCs |
| title_sort | optimizing conditions for labeling of mesenchymal stromal cells mscs with gold nanoparticles a prerequisite for in vivo tracking of mscs |
| topic | Mesenchymal stromal cells (MSCs) Au nanoparticles (Au NP), labeling, in vivo tracking |
| url | http://link.springer.com/article/10.1186/s12951-017-0258-5 |
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