Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind.
A tissue culture system was established from young leaves of in vitro grown seedling of Torenia fournieri Lind. Leafsegments were cultured on Murashige and Skoog medium (MS) medium supplemented with combinations of NAA and BA. Shoot organogenesis was observed in all growth regulators containing medi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Prince of Songkla University
2010-01-01
|
| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.rdoapp.psu.ac.th/html/sjst/journal/31-6/0125-3395-31-6-587-590.pdf |
| _version_ | 1798045131164614656 |
|---|---|
| author | Kantamaht Kanchanapoom Nirunya Buntin Kamnoon Kanchanapoom |
| author_facet | Kantamaht Kanchanapoom Nirunya Buntin Kamnoon Kanchanapoom |
| author_sort | Kantamaht Kanchanapoom |
| collection | DOAJ |
| description | A tissue culture system was established from young leaves of in vitro grown seedling of Torenia fournieri Lind. Leafsegments were cultured on Murashige and Skoog medium (MS) medium supplemented with combinations of NAA and BA. Shoot organogenesis was observed in all growth regulators containing medium. High frequency regeneration was obtained from leaves cultured on MS medium supplemented with 0.05 mg/l NAA plus 3 mg/l BA. When individual isolated shoots were transferred to MS medium devoid of growth regulators, complete plantlets were obtained. Adventitious shoots were also regenerated from callus that was derived from leaf explants and a high frequency of shoot organogenesis was found on MSmedium containing 0.1 mg/l NAA plus 3 mg/l BA. |
| first_indexed | 2024-04-11T23:15:30Z |
| format | Article |
| id | doaj.art-99cc536bb077413fae83d6d5a97bce8a |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-04-11T23:15:30Z |
| publishDate | 2010-01-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-99cc536bb077413fae83d6d5a97bce8a2022-12-22T03:57:38ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952010-01-01316587590Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind.Kantamaht KanchanapoomNirunya BuntinKamnoon KanchanapoomA tissue culture system was established from young leaves of in vitro grown seedling of Torenia fournieri Lind. Leafsegments were cultured on Murashige and Skoog medium (MS) medium supplemented with combinations of NAA and BA. Shoot organogenesis was observed in all growth regulators containing medium. High frequency regeneration was obtained from leaves cultured on MS medium supplemented with 0.05 mg/l NAA plus 3 mg/l BA. When individual isolated shoots were transferred to MS medium devoid of growth regulators, complete plantlets were obtained. Adventitious shoots were also regenerated from callus that was derived from leaf explants and a high frequency of shoot organogenesis was found on MSmedium containing 0.1 mg/l NAA plus 3 mg/l BA.http://www.rdoapp.psu.ac.th/html/sjst/journal/31-6/0125-3395-31-6-587-590.pdfadventitious organogenesisleaf culturemicropropagationTorenia fournieriWishbone flower |
| spellingShingle | Kantamaht Kanchanapoom Nirunya Buntin Kamnoon Kanchanapoom Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind. Songklanakarin Journal of Science and Technology (SJST) adventitious organogenesis leaf culture micropropagation Torenia fournieri Wishbone flower |
| title | Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind. |
| title_full | Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind. |
| title_fullStr | Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind. |
| title_full_unstemmed | Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind. |
| title_short | Micropropagation through adventitious shoot regeneration from leaf culture of Torenia fournieri Lind. |
| title_sort | micropropagation through adventitious shoot regeneration from leaf culture of torenia fournieri lind |
| topic | adventitious organogenesis leaf culture micropropagation Torenia fournieri Wishbone flower |
| url | http://www.rdoapp.psu.ac.th/html/sjst/journal/31-6/0125-3395-31-6-587-590.pdf |
| work_keys_str_mv | AT kantamahtkanchanapoom micropropagationthroughadventitiousshootregenerationfromleafcultureoftoreniafournierilind AT nirunyabuntin micropropagationthroughadventitiousshootregenerationfromleafcultureoftoreniafournierilind AT kamnoonkanchanapoom micropropagationthroughadventitiousshootregenerationfromleafcultureoftoreniafournierilind |