HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims
Neurological dysfunction is not uncommon in patients suffering from acquired immunodeficiency syndrome (AIDS) and, when manifested, intimates involvement of the central nervous system. Here, the human immunodeficiency virus (HIV) infects preferentially microglial cells, which thereby release substan...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1997-01-01
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| Series: | Neurobiology of Disease |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996197901431 |
| _version_ | 1818869983807012864 |
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| author | Pavel V. Belichenko Judith Miklossy Marco R. Celio |
| author_facet | Pavel V. Belichenko Judith Miklossy Marco R. Celio |
| author_sort | Pavel V. Belichenko |
| collection | DOAJ |
| description | Neurological dysfunction is not uncommon in patients suffering from acquired immunodeficiency syndrome (AIDS) and, when manifested, intimates involvement of the central nervous system. Here, the human immunodeficiency virus (HIV) infects preferentially microglial cells, which thereby release substances known to interfere with neuronal function. One class of agents set free in this manner are proteases; these degrade certain components within, and thereby undermine the integrity of, the extracellular matrix (ECM) compartment, which plays a vital role in cell-to-cell communication. We wished to ascertain whether the ECM compartment is indeed disrupted in the brains of AIDS victims. We examined the neocortical areas of 27 AIDS autopsy cases, including 9 with diagnosed HIV-encephalopathy (HIVE); 8 HIV-seronegative cases with various types of brain lesion, including viral infections, were also included in this study. HIV-antigens and DNA were identified by use of immunohistochemistry andin situhybridization, and ECM components by lectin staining and immunohistochemistry. Of the 27 AIDS cases examined, each of the 9 with HIVE was completely devoid of labeled ECM components; 8 of the 18 without HIVE had incurred substantial losses, and only 2 manifested a normal complement of constituents within this compartment. With respect to stratal and topographic variations, layers II and III were less affected than layers V to VII, as was the frontal cortex relative to other areas. These findings confirmed our expectations of the brain's ECM undergoing degradation following HIV infection, and these changes may well underlie the neurological disturbances manifested in AIDS patients. |
| first_indexed | 2024-12-19T11:59:48Z |
| format | Article |
| id | doaj.art-99d111ce15404de58823bac46caa5d18 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-19T11:59:48Z |
| publishDate | 1997-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-99d111ce15404de58823bac46caa5d182022-12-21T20:22:31ZengElsevierNeurobiology of Disease1095-953X1997-01-0143301310HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS VictimsPavel V. Belichenko0Judith Miklossy1Marco R. Celio2Institute of Histology and General Embryology, University of Fribourg, CH-1700, Fribourg, Switzerland; Brain Research Institute, Russian Academy of Medical Sciences, Moscow, 103064, Russia; University Institute of Pathology, Division of Neuropathology, University of Lausanne, CH-1011, Lausanne, SwitzerlandInstitute of Histology and General Embryology, University of Fribourg, CH-1700, Fribourg, Switzerland; Brain Research Institute, Russian Academy of Medical Sciences, Moscow, 103064, Russia; University Institute of Pathology, Division of Neuropathology, University of Lausanne, CH-1011, Lausanne, SwitzerlandInstitute of Histology and General Embryology, University of Fribourg, CH-1700, Fribourg, Switzerland; Brain Research Institute, Russian Academy of Medical Sciences, Moscow, 103064, Russia; University Institute of Pathology, Division of Neuropathology, University of Lausanne, CH-1011, Lausanne, SwitzerlandNeurological dysfunction is not uncommon in patients suffering from acquired immunodeficiency syndrome (AIDS) and, when manifested, intimates involvement of the central nervous system. Here, the human immunodeficiency virus (HIV) infects preferentially microglial cells, which thereby release substances known to interfere with neuronal function. One class of agents set free in this manner are proteases; these degrade certain components within, and thereby undermine the integrity of, the extracellular matrix (ECM) compartment, which plays a vital role in cell-to-cell communication. We wished to ascertain whether the ECM compartment is indeed disrupted in the brains of AIDS victims. We examined the neocortical areas of 27 AIDS autopsy cases, including 9 with diagnosed HIV-encephalopathy (HIVE); 8 HIV-seronegative cases with various types of brain lesion, including viral infections, were also included in this study. HIV-antigens and DNA were identified by use of immunohistochemistry andin situhybridization, and ECM components by lectin staining and immunohistochemistry. Of the 27 AIDS cases examined, each of the 9 with HIVE was completely devoid of labeled ECM components; 8 of the 18 without HIVE had incurred substantial losses, and only 2 manifested a normal complement of constituents within this compartment. With respect to stratal and topographic variations, layers II and III were less affected than layers V to VII, as was the frontal cortex relative to other areas. These findings confirmed our expectations of the brain's ECM undergoing degradation following HIV infection, and these changes may well underlie the neurological disturbances manifested in AIDS patients.http://www.sciencedirect.com/science/article/pii/S0969996197901431 |
| spellingShingle | Pavel V. Belichenko Judith Miklossy Marco R. Celio HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims Neurobiology of Disease |
| title | HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims |
| title_full | HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims |
| title_fullStr | HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims |
| title_full_unstemmed | HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims |
| title_short | HIV-I Induced Destruction of Neocortical Extracellular Matrix Components in AIDS Victims |
| title_sort | hiv i induced destruction of neocortical extracellular matrix components in aids victims |
| url | http://www.sciencedirect.com/science/article/pii/S0969996197901431 |
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