THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION
Aim. In the present work we investigated the circulation of AIV in wild bird populations and studied the sorption of the influenza virus in the feathers of wild waterfowl nesting on reservoirs during the autumn mass migration. Material and methods. Sampling was carried out on the territory of the No...
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| Format: | Article |
| Language: | Russian |
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Kamerton
2018-10-01
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| Series: | Юг России: экология, развитие |
| Subjects: | |
| Online Access: | https://ecodag.elpub.ru/ugro/article/view/1308 |
| _version_ | 1826562498037284864 |
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| author | Marin A. Gulyaeva Kirill A. Sharshov Ivan A. Sobolev Alexander K. Yurlov Alimurad A. Gadzhiev Nukhkadi I. Rabazanov Lidia V. Shestopalova Alexander M. Shestopalov |
| author_facet | Marin A. Gulyaeva Kirill A. Sharshov Ivan A. Sobolev Alexander K. Yurlov Alimurad A. Gadzhiev Nukhkadi I. Rabazanov Lidia V. Shestopalova Alexander M. Shestopalov |
| author_sort | Marin A. Gulyaeva |
| collection | DOAJ |
| description | Aim. In the present work we investigated the circulation of AIV in wild bird populations and studied the sorption of the influenza virus in the feathers of wild waterfowl nesting on reservoirs during the autumn mass migration. Material and methods. Sampling was carried out on the territory of the Novosibirsk region on Lake Chany during the period from August to September 2014-2016. Biological samples were collected from 188 wild waterfowl of various species. AIV isolation from cloacal swabs and swabs collected from feathers was carried out in the developing chick embryo system (RCC) as previously recommended. The isolated viruses were tested by HA/HI with specific sera, PCR analysis was carried out with subtyping primers. The genomes of the isolated viruses were sequenced for phylogenetic analysis. Results and discussion. As a result of monitoring, cloacal and feather swabs were collected from 188 individuals belonging to 13 species of the Anseriformes and Charadriiformes, whose representatives are the main natural reservoir of AIV. Fifteen new AI viruses were isolated from the collected samples. Four of them were isolated from plumage samples and the rate was more than 2 times lower, compared with virus isolation from cloacal swabs. Main conclusions. Thus, it can be assumed that avian influenza virus transmission by plumage during migration is not sufficiently taken into account. The key role in AIV ecology may play the virus spreading by its adsorption on bird feathers. |
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| id | doaj.art-99d1f6e9fbcd4dee881eb09943d1ed03 |
| institution | Directory Open Access Journal |
| issn | 1992-1098 2413-0958 |
| language | Russian |
| last_indexed | 2025-03-14T09:49:01Z |
| publishDate | 2018-10-01 |
| publisher | Kamerton |
| record_format | Article |
| series | Юг России: экология, развитие |
| spelling | doaj.art-99d1f6e9fbcd4dee881eb09943d1ed032025-03-02T11:27:02ZrusKamertonЮг России: экология, развитие1992-10982413-09582018-10-0113313414110.18470/1992-1098-2018-3-134-1411008THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATIONMarin A. Gulyaeva0Kirill A. Sharshov1Ivan A. Sobolev2Alexander K. Yurlov3Alimurad A. Gadzhiev4Nukhkadi I. Rabazanov5Lidia V. Shestopalova6Alexander M. Shestopalov7Research Institute of Experimental and Clinical Medicine, Novosibirsk; Novosibirsk State University, NovosibirskFederal Research Center of Fundamental and Translational Medicine, NovosibirskResearch Institute of Experimental and Clinical Medicine, NovosibirskInstitute of Animal Systematics and Ecology of the SB RAS, NovosibirskDagestan State University, MakhachkalaDagestan State University, Makhachkala; Сaspian Institute of Biological Resources of DSC Russian Academy of Sciences, MakhachkalaNovosibirsk State University, NovosibirskResearch Institute of Experimental and Clinical Medicine, NovosibirskAim. In the present work we investigated the circulation of AIV in wild bird populations and studied the sorption of the influenza virus in the feathers of wild waterfowl nesting on reservoirs during the autumn mass migration. Material and methods. Sampling was carried out on the territory of the Novosibirsk region on Lake Chany during the period from August to September 2014-2016. Biological samples were collected from 188 wild waterfowl of various species. AIV isolation from cloacal swabs and swabs collected from feathers was carried out in the developing chick embryo system (RCC) as previously recommended. The isolated viruses were tested by HA/HI with specific sera, PCR analysis was carried out with subtyping primers. The genomes of the isolated viruses were sequenced for phylogenetic analysis. Results and discussion. As a result of monitoring, cloacal and feather swabs were collected from 188 individuals belonging to 13 species of the Anseriformes and Charadriiformes, whose representatives are the main natural reservoir of AIV. Fifteen new AI viruses were isolated from the collected samples. Four of them were isolated from plumage samples and the rate was more than 2 times lower, compared with virus isolation from cloacal swabs. Main conclusions. Thus, it can be assumed that avian influenza virus transmission by plumage during migration is not sufficiently taken into account. The key role in AIV ecology may play the virus spreading by its adsorption on bird feathers.https://ecodag.elpub.ru/ugro/article/view/1308plumageavian influenza virusbird ecologyways of virus transmission |
| spellingShingle | Marin A. Gulyaeva Kirill A. Sharshov Ivan A. Sobolev Alexander K. Yurlov Alimurad A. Gadzhiev Nukhkadi I. Rabazanov Lidia V. Shestopalova Alexander M. Shestopalov THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION Юг России: экология, развитие plumage avian influenza virus bird ecology ways of virus transmission |
| title | THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION |
| title_full | THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION |
| title_fullStr | THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION |
| title_full_unstemmed | THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION |
| title_short | THE ISOLATION OF INFLUENZA A VIRUS FROM PLUMAGE OF WATERFOWL DURING AUTUMN MIGRATION |
| title_sort | isolation of influenza a virus from plumage of waterfowl during autumn migration |
| topic | plumage avian influenza virus bird ecology ways of virus transmission |
| url | https://ecodag.elpub.ru/ugro/article/view/1308 |
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