Ameliorative effect of carveol on scopolamine induced memory impairment in rats
Objective(s): Carveol is a naturally occurring terpenoid with antispasmodic, carminative, astringent, indigestion, and dyspepsia properties, as well as anti-diabetic, anti-oxidant, anti-hyperlipidemia, and anti-inflammatory properties in the liver. Research also suggests that it has memory-enhancing...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_21182_54ee0ac9f494cd54c8cca16d423410a5.pdf |
| _version_ | 1797977635428499456 |
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| author | Komal Latif Saneela Saneela Arif-ullah Khan |
| author_facet | Komal Latif Saneela Saneela Arif-ullah Khan |
| author_sort | Komal Latif |
| collection | DOAJ |
| description | Objective(s): Carveol is a naturally occurring terpenoid with antispasmodic, carminative, astringent, indigestion, and dyspepsia properties, as well as anti-diabetic, anti-oxidant, anti-hyperlipidemia, and anti-inflammatory properties in the liver. Research also suggests that it has memory-enhancing and anti-oxidant properties. The purpose of this research was to see whether carveol could protect rats against scopolamine-induced memory loss in a rat model. Materials and Methods: Thirty male Sprague-Dawley rats (200–250 g) were grouped as the saline group receiving saline, disease group receiving scopolamine, and four treatment groups among which three groups received scopalamine+carveol and one group received scopalamine+donepezil for 28 days. Followed by in vitro, behavioral, anti-oxidant, and molecular studies were done. P<0.005 was considered statistically significant.Results: The in vitro assay showed that carveol caused diphenyl-1-picrylhydrazyl inhibition. In-vivo findings revealed that carveol (50, 100, and 200 mg/kg) significantly improved dementia by reducing escape latency and spending more time in the targeted quadrant in the Morris water maze test. Increased number of entries and percent spontaneous alterations were observed in rats’ Y-maze test. In animal brain tissues, i.e., cortex and hippocampus, carveol enhanced glutathione, glutathione-s-transferase, catalase, and reduced lipid peroxide levels. Carveol also improved cellular architecture in histopathological examinations and decreased expression of inflammatory markers such as amyloid-beta, nuclear factor kappa light chain activated B cells, tumor necrosis factor-alpha, cyclooxygenase 2, prostaglandin E2, and interleukin-18, as evidenced by immunohistochemistry and enzyme-linked immunosorbent assays, as well as molecular investigations. Conclusion: This study suggests that the compound could be potent against amnesia mediated through anti-oxidant, amyloid-beta inhibition, and anti-inflammatory pathways. |
| first_indexed | 2024-04-11T05:10:57Z |
| format | Article |
| id | doaj.art-99d5c4a88c8c4510af1036ecf7cfb33a |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T05:10:57Z |
| publishDate | 2022-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-99d5c4a88c8c4510af1036ecf7cfb33a2022-12-25T11:14:14ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-12-0125121504151210.22038/ijbms.2022.66797.1464721182Ameliorative effect of carveol on scopolamine induced memory impairment in ratsKomal Latif0Saneela Saneela1Arif-ullah Khan2Riphah Institute of Pharmaceutical Sciences, Riphah International University, IslamabadRiphah Institute of Pharmaceutical Sciences, Riphah International University, IslamabadRiphah Institute of Pharmaceutical Sciences, Riphah International University, IslamabadObjective(s): Carveol is a naturally occurring terpenoid with antispasmodic, carminative, astringent, indigestion, and dyspepsia properties, as well as anti-diabetic, anti-oxidant, anti-hyperlipidemia, and anti-inflammatory properties in the liver. Research also suggests that it has memory-enhancing and anti-oxidant properties. The purpose of this research was to see whether carveol could protect rats against scopolamine-induced memory loss in a rat model. Materials and Methods: Thirty male Sprague-Dawley rats (200–250 g) were grouped as the saline group receiving saline, disease group receiving scopolamine, and four treatment groups among which three groups received scopalamine+carveol and one group received scopalamine+donepezil for 28 days. Followed by in vitro, behavioral, anti-oxidant, and molecular studies were done. P<0.005 was considered statistically significant.Results: The in vitro assay showed that carveol caused diphenyl-1-picrylhydrazyl inhibition. In-vivo findings revealed that carveol (50, 100, and 200 mg/kg) significantly improved dementia by reducing escape latency and spending more time in the targeted quadrant in the Morris water maze test. Increased number of entries and percent spontaneous alterations were observed in rats’ Y-maze test. In animal brain tissues, i.e., cortex and hippocampus, carveol enhanced glutathione, glutathione-s-transferase, catalase, and reduced lipid peroxide levels. Carveol also improved cellular architecture in histopathological examinations and decreased expression of inflammatory markers such as amyloid-beta, nuclear factor kappa light chain activated B cells, tumor necrosis factor-alpha, cyclooxygenase 2, prostaglandin E2, and interleukin-18, as evidenced by immunohistochemistry and enzyme-linked immunosorbent assays, as well as molecular investigations. Conclusion: This study suggests that the compound could be potent against amnesia mediated through anti-oxidant, amyloid-beta inhibition, and anti-inflammatory pathways.https://ijbms.mums.ac.ir/article_21182_54ee0ac9f494cd54c8cca16d423410a5.pdfalzheimeramnesiaanti-inflammatoryanti-oxidantcarveolmemory impairmentneuroinflammation |
| spellingShingle | Komal Latif Saneela Saneela Arif-ullah Khan Ameliorative effect of carveol on scopolamine induced memory impairment in rats Iranian Journal of Basic Medical Sciences alzheimer amnesia anti-inflammatory anti-oxidant carveol memory impairment neuroinflammation |
| title | Ameliorative effect of carveol on scopolamine induced memory impairment in rats |
| title_full | Ameliorative effect of carveol on scopolamine induced memory impairment in rats |
| title_fullStr | Ameliorative effect of carveol on scopolamine induced memory impairment in rats |
| title_full_unstemmed | Ameliorative effect of carveol on scopolamine induced memory impairment in rats |
| title_short | Ameliorative effect of carveol on scopolamine induced memory impairment in rats |
| title_sort | ameliorative effect of carveol on scopolamine induced memory impairment in rats |
| topic | alzheimer amnesia anti-inflammatory anti-oxidant carveol memory impairment neuroinflammation |
| url | https://ijbms.mums.ac.ir/article_21182_54ee0ac9f494cd54c8cca16d423410a5.pdf |
| work_keys_str_mv | AT komallatif ameliorativeeffectofcarveolonscopolamineinducedmemoryimpairmentinrats AT saneelasaneela ameliorativeeffectofcarveolonscopolamineinducedmemoryimpairmentinrats AT arifullahkhan ameliorativeeffectofcarveolonscopolamineinducedmemoryimpairmentinrats |