Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil
The kinetics and thermodynamics of the enzymatic degradation of zearalenone (ZEN) in degummed corn oil were investigated by analyzing the impacts of temperature, pH, ZEN hydrolase dosage and ZEN concentration on the initial reaction rate. The kinetic study found that the maximum reaction rate was 0....
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/1/19 |
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| author | Chenwei Zhao Pengkai Xie Jun Jin Qingzhe Jin Xingguo Wang |
| author_facet | Chenwei Zhao Pengkai Xie Jun Jin Qingzhe Jin Xingguo Wang |
| author_sort | Chenwei Zhao |
| collection | DOAJ |
| description | The kinetics and thermodynamics of the enzymatic degradation of zearalenone (ZEN) in degummed corn oil were investigated by analyzing the impacts of temperature, pH, ZEN hydrolase dosage and ZEN concentration on the initial reaction rate. The kinetic study found that the maximum reaction rate was 0.97 μmol × kg<sup>−1</sup> min<sup>−1</sup>, the Michaelis constant (Km) was 11,476 μmol × kg<sup>−1</sup> and the Michaelis equation was V = 0.97[S]/(11,476 + [S]). The thermodynamic study showed that the activation energy (Ea) was 70.37 kJ·mol<sup>−1</sup>, the activation enthalpy change of the reaction (ΔH) > 0, the free energy of activation (ΔG) > 0 and the activation entropy change (ΔS) < 0, indicating the reaction could not be spontaneous. The reaction mechanism of ZEN was studied by a hybrid quadrupole orbitrap mass spectrometer. It was found that ZEN first generated the intermediate G/L/D/W-ZEN+H<sub>2</sub>O, followed by generating the intermediate W-ZEN-H<sub>2</sub>O under the action of a degrading enzyme. Then, the lactone bond was opened to produce C<sub>18</sub>H<sub>24</sub>O<sub>6</sub>, and finally the decarboxylation product C<sub>17</sub>H<sub>24</sub>O<sub>4</sub> formed automatically. |
| first_indexed | 2024-03-09T11:06:20Z |
| format | Article |
| id | doaj.art-99d60549951c473485b91618ff24209c |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T11:06:20Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-99d60549951c473485b91618ff24209c2023-12-01T00:56:34ZengMDPI AGToxins2072-66512022-12-011511910.3390/toxins15010019Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn OilChenwei Zhao0Pengkai Xie1Jun Jin2Qingzhe Jin3Xingguo Wang4School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaThe kinetics and thermodynamics of the enzymatic degradation of zearalenone (ZEN) in degummed corn oil were investigated by analyzing the impacts of temperature, pH, ZEN hydrolase dosage and ZEN concentration on the initial reaction rate. The kinetic study found that the maximum reaction rate was 0.97 μmol × kg<sup>−1</sup> min<sup>−1</sup>, the Michaelis constant (Km) was 11,476 μmol × kg<sup>−1</sup> and the Michaelis equation was V = 0.97[S]/(11,476 + [S]). The thermodynamic study showed that the activation energy (Ea) was 70.37 kJ·mol<sup>−1</sup>, the activation enthalpy change of the reaction (ΔH) > 0, the free energy of activation (ΔG) > 0 and the activation entropy change (ΔS) < 0, indicating the reaction could not be spontaneous. The reaction mechanism of ZEN was studied by a hybrid quadrupole orbitrap mass spectrometer. It was found that ZEN first generated the intermediate G/L/D/W-ZEN+H<sub>2</sub>O, followed by generating the intermediate W-ZEN-H<sub>2</sub>O under the action of a degrading enzyme. Then, the lactone bond was opened to produce C<sub>18</sub>H<sub>24</sub>O<sub>6</sub>, and finally the decarboxylation product C<sub>17</sub>H<sub>24</sub>O<sub>4</sub> formed automatically.https://www.mdpi.com/2072-6651/15/1/19zearalenonehydrolasedegummed corn oilkineticsthermodynamicmechanism |
| spellingShingle | Chenwei Zhao Pengkai Xie Jun Jin Qingzhe Jin Xingguo Wang Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil Toxins zearalenone hydrolase degummed corn oil kinetics thermodynamic mechanism |
| title | Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil |
| title_full | Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil |
| title_fullStr | Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil |
| title_full_unstemmed | Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil |
| title_short | Kinetics, Thermodynamics and Mechanism of Enzymatic Degradation of Zearalenone in Degummed Corn Oil |
| title_sort | kinetics thermodynamics and mechanism of enzymatic degradation of zearalenone in degummed corn oil |
| topic | zearalenone hydrolase degummed corn oil kinetics thermodynamic mechanism |
| url | https://www.mdpi.com/2072-6651/15/1/19 |
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