Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells
Corneal wound healing involves communication between the different cell types that constitute the three cellular layers of the cornea (epithelium, stroma and endothelium), a process ensured in part by a category of extracellular vesicles called exosomes. In the present study, we isolated exosomes re...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12201 |
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| author | Pascale Desjardins Rébecca Berthiaume Camille Couture Gaëtan Le-Bel Vincent Roy François Gros-Louis Véronique J. Moulin Stéphanie Proulx Sylvain Chemtob Lucie Germain Sylvain L. Guérin |
| author_facet | Pascale Desjardins Rébecca Berthiaume Camille Couture Gaëtan Le-Bel Vincent Roy François Gros-Louis Véronique J. Moulin Stéphanie Proulx Sylvain Chemtob Lucie Germain Sylvain L. Guérin |
| author_sort | Pascale Desjardins |
| collection | DOAJ |
| description | Corneal wound healing involves communication between the different cell types that constitute the three cellular layers of the cornea (epithelium, stroma and endothelium), a process ensured in part by a category of extracellular vesicles called exosomes. In the present study, we isolated exosomes released by primary cultured human corneal epithelial cells (hCECs), corneal fibroblasts (hCFs) and corneal endothelial cells (hCEnCs) and determined whether they have wound healing characteristics of their own and to which point they modify the genetic and proteomic pattern of these cell types. Exosomes released by all three cell types significantly accelerated wound closure of scratch-wounded hCECs in vitro compared to controls (without exosomes). Profiling of activated kinases revealed that exosomes from human corneal cells caused the activation of signal transduction mediators that belong to the HSP27, STAT, β-catenin, GSK-3β and p38 pathways. Most of all, data from gene profiling analyses indicated that exosomes, irrespective of their cellular origin, alter a restricted subset of genes that are completely different between each targeted cell type (hCECs, hCFS, hCEnCs). Analysis of the genes specifically differentially regulated for a given cell-type in the microarray data using the Ingenuity Pathway Analysis (IPA) software revealed that the mean gene expression profile of hCECs cultured in the presence of exosomes would likely promote cell proliferation and migration whereas it would reduce differentiation when compared to control cells. Collectively, our findings represent a conceptual advance in understanding the mechanisms of corneal wound repair that may ultimately open new avenues for the development of novel therapeutic approaches to improve closure of corneal wounds. |
| first_indexed | 2024-03-09T09:47:05Z |
| format | Article |
| id | doaj.art-99dbce568d9043bb818f8fb96b3711eb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:47:05Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-99dbce568d9043bb818f8fb96b3711eb2023-12-02T00:31:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201220110.3390/ijms232012201Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial CellsPascale Desjardins0Rébecca Berthiaume1Camille Couture2Gaëtan Le-Bel3Vincent Roy4François Gros-Louis5Véronique J. Moulin6Stéphanie Proulx7Sylvain Chemtob8Lucie Germain9Sylvain L. Guérin10Regenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaDépartement d’Ophtalmologie, Faculté de Médecine, Université de Montréal, Montréal, QC H3T 1J4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaRegenerative Medicine Division of the Centre de Recherche du CHU de Québec, Université Laval, Québec, QC G1J 1Z4, CanadaCorneal wound healing involves communication between the different cell types that constitute the three cellular layers of the cornea (epithelium, stroma and endothelium), a process ensured in part by a category of extracellular vesicles called exosomes. In the present study, we isolated exosomes released by primary cultured human corneal epithelial cells (hCECs), corneal fibroblasts (hCFs) and corneal endothelial cells (hCEnCs) and determined whether they have wound healing characteristics of their own and to which point they modify the genetic and proteomic pattern of these cell types. Exosomes released by all three cell types significantly accelerated wound closure of scratch-wounded hCECs in vitro compared to controls (without exosomes). Profiling of activated kinases revealed that exosomes from human corneal cells caused the activation of signal transduction mediators that belong to the HSP27, STAT, β-catenin, GSK-3β and p38 pathways. Most of all, data from gene profiling analyses indicated that exosomes, irrespective of their cellular origin, alter a restricted subset of genes that are completely different between each targeted cell type (hCECs, hCFS, hCEnCs). Analysis of the genes specifically differentially regulated for a given cell-type in the microarray data using the Ingenuity Pathway Analysis (IPA) software revealed that the mean gene expression profile of hCECs cultured in the presence of exosomes would likely promote cell proliferation and migration whereas it would reduce differentiation when compared to control cells. Collectively, our findings represent a conceptual advance in understanding the mechanisms of corneal wound repair that may ultimately open new avenues for the development of novel therapeutic approaches to improve closure of corneal wounds.https://www.mdpi.com/1422-0067/23/20/12201corneawound healingexosomesextracellular vesiclescorneal epithelial cellscorneal stromal fibroblasts |
| spellingShingle | Pascale Desjardins Rébecca Berthiaume Camille Couture Gaëtan Le-Bel Vincent Roy François Gros-Louis Véronique J. Moulin Stéphanie Proulx Sylvain Chemtob Lucie Germain Sylvain L. Guérin Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells International Journal of Molecular Sciences cornea wound healing exosomes extracellular vesicles corneal epithelial cells corneal stromal fibroblasts |
| title | Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells |
| title_full | Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells |
| title_fullStr | Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells |
| title_full_unstemmed | Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells |
| title_short | Impact of Exosomes Released by Different Corneal Cell Types on the Wound Healing Properties of Human Corneal Epithelial Cells |
| title_sort | impact of exosomes released by different corneal cell types on the wound healing properties of human corneal epithelial cells |
| topic | cornea wound healing exosomes extracellular vesicles corneal epithelial cells corneal stromal fibroblasts |
| url | https://www.mdpi.com/1422-0067/23/20/12201 |
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