The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers
This article presents the results of the start-up of continuous production of biohydrogen from cheese whey (CW) in an anaerobic filter (AF) and anaerobic fluidized bed (AFB) with a polyurethane carrier. Heat and acid pretreatments were used for the inactivation of hydrogen-scavengers in the inoculum...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/2/510 |
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| author | Elza R. Mikheeva Inna V. Katraeva Andrey A. Kovalev Dmitriy A. Kovalev Alla N. Nozhevnikova Vladimir Panchenko Ugo Fiore Yuri V. Litti |
| author_facet | Elza R. Mikheeva Inna V. Katraeva Andrey A. Kovalev Dmitriy A. Kovalev Alla N. Nozhevnikova Vladimir Panchenko Ugo Fiore Yuri V. Litti |
| author_sort | Elza R. Mikheeva |
| collection | DOAJ |
| description | This article presents the results of the start-up of continuous production of biohydrogen from cheese whey (CW) in an anaerobic filter (AF) and anaerobic fluidized bed (AFB) with a polyurethane carrier. Heat and acid pretreatments were used for the inactivation of hydrogen-scavengers in the inoculum (mesophilic and thermophilic anaerobic sludge). Acid pretreatment was effective for thermophilic anaerobic sludge to suppress methanogenic activity, and heat treatment was effective for mesophilic anaerobic sludge. Maximum specific yields of hydrogen, namely 178 mL/g chemical oxygen demand (COD) and 149 mL/g COD for AFB and AF, respectively, were obtained at the hydraulic retention time (HRT) of 4.5 days and organic load rate (OLR) of 6.61 kg COD/(m<sup>3</sup> day). At the same time, the maximum hydrogen production rates of 1.28 and 1.9 NL/(L day) for AF and AFB, respectively, were obtained at the HRT of 2.02 days and OLR of 14.88 kg COD/(m<sup>3</sup> day). At the phylum level, the dominant taxa were Firmicutes (65% in AF and 60% in AFB), and at the genus level, <i>Lactobacillus</i> (40% in AF and 43% in AFB) and <i>Bifidobacterium</i> (24% in AF and 30% in AFB). |
| first_indexed | 2024-03-09T05:52:25Z |
| format | Article |
| id | doaj.art-99dc4ea12b8e4a6fa9867dda31e4ecb3 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T05:52:25Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-99dc4ea12b8e4a6fa9867dda31e4ecb32023-12-03T12:16:28ZengMDPI AGApplied Sciences2076-34172021-01-0111251010.3390/app11020510The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane CarriersElza R. Mikheeva0Inna V. Katraeva1Andrey A. Kovalev2Dmitriy A. Kovalev3Alla N. Nozhevnikova4Vladimir Panchenko5Ugo Fiore6Yuri V. Litti7Laboratory of Resource-Saving Biotechnologies, Lobachevsky State University of Nizhni Novgorod, 603950 Nizhnij Novgorod, RussiaNizhny Novgorod State University of Architecture and Civil Engineering, 603950 Nizhnij Novgorod, RussiaDepartment of Renewable Energy, Federal Scientific Agroengineering Center VIM, 109428 Moscow, RussiaDepartment of Renewable Energy, Federal Scientific Agroengineering Center VIM, 109428 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, RussiaDepartment of Theoretical and Applied Mechanics, Russian University of Transport, 127994 Moscow, RussiaDepartment of Management and Quantitative Studies, Parthenope University, 80132 Naples, ItalyFederal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, RussiaThis article presents the results of the start-up of continuous production of biohydrogen from cheese whey (CW) in an anaerobic filter (AF) and anaerobic fluidized bed (AFB) with a polyurethane carrier. Heat and acid pretreatments were used for the inactivation of hydrogen-scavengers in the inoculum (mesophilic and thermophilic anaerobic sludge). Acid pretreatment was effective for thermophilic anaerobic sludge to suppress methanogenic activity, and heat treatment was effective for mesophilic anaerobic sludge. Maximum specific yields of hydrogen, namely 178 mL/g chemical oxygen demand (COD) and 149 mL/g COD for AFB and AF, respectively, were obtained at the hydraulic retention time (HRT) of 4.5 days and organic load rate (OLR) of 6.61 kg COD/(m<sup>3</sup> day). At the same time, the maximum hydrogen production rates of 1.28 and 1.9 NL/(L day) for AF and AFB, respectively, were obtained at the HRT of 2.02 days and OLR of 14.88 kg COD/(m<sup>3</sup> day). At the phylum level, the dominant taxa were Firmicutes (65% in AF and 60% in AFB), and at the genus level, <i>Lactobacillus</i> (40% in AF and 43% in AFB) and <i>Bifidobacterium</i> (24% in AF and 30% in AFB).https://www.mdpi.com/2076-3417/11/2/510biohydrogencheese wheydark fermentationinactivation of methanogensimmobilizationpolyurethane foam |
| spellingShingle | Elza R. Mikheeva Inna V. Katraeva Andrey A. Kovalev Dmitriy A. Kovalev Alla N. Nozhevnikova Vladimir Panchenko Ugo Fiore Yuri V. Litti The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers Applied Sciences biohydrogen cheese whey dark fermentation inactivation of methanogens immobilization polyurethane foam |
| title | The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers |
| title_full | The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers |
| title_fullStr | The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers |
| title_full_unstemmed | The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers |
| title_short | The Start-Up of Continuous Biohydrogen Production from Cheese Whey: Comparison of Inoculum Pretreatment Methods and Reactors with Moving and Fixed Polyurethane Carriers |
| title_sort | start up of continuous biohydrogen production from cheese whey comparison of inoculum pretreatment methods and reactors with moving and fixed polyurethane carriers |
| topic | biohydrogen cheese whey dark fermentation inactivation of methanogens immobilization polyurethane foam |
| url | https://www.mdpi.com/2076-3417/11/2/510 |
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