Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells
Type I and III interferons are among the most important antiviral mediators. Increased susceptibility to infections has been described as being associated with impaired interferon response in asthmatic patients. In this work, we focused on the modulation of interferon dysfunction after the rhinoviru...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/12/2328 |
| _version_ | 1797379163920793600 |
|---|---|
| author | Hannah Mitländer Zuqin Yang Susanne Krammer Janina C. Grund Sabine Zirlik Susetta Finotto |
| author_facet | Hannah Mitländer Zuqin Yang Susanne Krammer Janina C. Grund Sabine Zirlik Susetta Finotto |
| author_sort | Hannah Mitländer |
| collection | DOAJ |
| description | Type I and III interferons are among the most important antiviral mediators. Increased susceptibility to infections has been described as being associated with impaired interferon response in asthmatic patients. In this work, we focused on the modulation of interferon dysfunction after the rhinovirus infection of airway epithelial cells. Therefore, we tested polyinosinic:polycytidylic acid (poly I:C), a TLR3 agonist, as a possible preventive pre-treatment to improve this anti-viral response. In our human study on asthma, we found a deficiency in interferon levels in the nasal epithelial cells (NEC) from asthmatics at homeostatic level and after RV infection, which might contribute to frequent airway infection seen in asthmatic patients compared to healthy controls. Finally, pre-treatment with the immunomodulatory substance poly I:C before RV infection restored IFN responses in airway epithelial cells. Altogether, we consider poly I:C pre-treatment as a promising strategy for the induction of interferon response prior to viral infections. These results might help to improve current therapeutic strategies for allergic asthma exacerbations. |
| first_indexed | 2024-03-08T20:17:22Z |
| format | Article |
| id | doaj.art-99ddc906efe64372960e09d330c702a0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-08T20:17:22Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-99ddc906efe64372960e09d330c702a02023-12-22T14:49:06ZengMDPI AGViruses1999-49152023-11-011512232810.3390/v15122328Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial CellsHannah Mitländer0Zuqin Yang1Susanne Krammer2Janina C. Grund3Sabine Zirlik4Susetta Finotto5Department of Molecular Pneumology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, 91054 Erlangen, GermanyDepartment of Molecular Pneumology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, 91054 Erlangen, GermanyDepartment of Molecular Pneumology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, 91054 Erlangen, GermanyDepartment of Molecular Pneumology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, 91054 Erlangen, GermanyDepartment of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, 91054 Erlangen, GermanyDepartment of Molecular Pneumology, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg, Universitätsklinikum Erlangen, 91054 Erlangen, GermanyType I and III interferons are among the most important antiviral mediators. Increased susceptibility to infections has been described as being associated with impaired interferon response in asthmatic patients. In this work, we focused on the modulation of interferon dysfunction after the rhinovirus infection of airway epithelial cells. Therefore, we tested polyinosinic:polycytidylic acid (poly I:C), a TLR3 agonist, as a possible preventive pre-treatment to improve this anti-viral response. In our human study on asthma, we found a deficiency in interferon levels in the nasal epithelial cells (NEC) from asthmatics at homeostatic level and after RV infection, which might contribute to frequent airway infection seen in asthmatic patients compared to healthy controls. Finally, pre-treatment with the immunomodulatory substance poly I:C before RV infection restored IFN responses in airway epithelial cells. Altogether, we consider poly I:C pre-treatment as a promising strategy for the induction of interferon response prior to viral infections. These results might help to improve current therapeutic strategies for allergic asthma exacerbations.https://www.mdpi.com/1999-4915/15/12/2328rhinovirusasthmaepithelial cellsinterferonspoly I:C |
| spellingShingle | Hannah Mitländer Zuqin Yang Susanne Krammer Janina C. Grund Sabine Zirlik Susetta Finotto Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells Viruses rhinovirus asthma epithelial cells interferons poly I:C |
| title | Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells |
| title_full | Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells |
| title_fullStr | Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells |
| title_full_unstemmed | Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells |
| title_short | Poly I:C Pre-Treatment Induced the Anti-Viral Interferon Response in Airway Epithelial Cells |
| title_sort | poly i c pre treatment induced the anti viral interferon response in airway epithelial cells |
| topic | rhinovirus asthma epithelial cells interferons poly I:C |
| url | https://www.mdpi.com/1999-4915/15/12/2328 |
| work_keys_str_mv | AT hannahmitlander polyicpretreatmentinducedtheantiviralinterferonresponseinairwayepithelialcells AT zuqinyang polyicpretreatmentinducedtheantiviralinterferonresponseinairwayepithelialcells AT susannekrammer polyicpretreatmentinducedtheantiviralinterferonresponseinairwayepithelialcells AT janinacgrund polyicpretreatmentinducedtheantiviralinterferonresponseinairwayepithelialcells AT sabinezirlik polyicpretreatmentinducedtheantiviralinterferonresponseinairwayepithelialcells AT susettafinotto polyicpretreatmentinducedtheantiviralinterferonresponseinairwayepithelialcells |