Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats
Background/Aims: This study aimed to investigate the difference among the expression of serotonin receptors (5-HT3, 5-HT4, and 5-HT7receptors) in colonic tissue of chronic diarrhea rats. Materials and Methods: A rat model of chronic diarrhea was established by lactose diet. The expression of 5-HT3,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | The Saudi Journal of Gastroenterology |
| Subjects: | |
| Online Access: | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2016;volume=22;issue=3;spage=234;epage=239;aulast=Zhu |
| _version_ | 1819036605939187712 |
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| author | Tong Zhu Juanjuan Qiu Jiajia Wan Fengyun Wang Xudong Tang Huishu Guo |
| author_facet | Tong Zhu Juanjuan Qiu Jiajia Wan Fengyun Wang Xudong Tang Huishu Guo |
| author_sort | Tong Zhu |
| collection | DOAJ |
| description | Background/Aims: This study aimed to investigate the difference among the expression of serotonin receptors (5-HT3, 5-HT4, and 5-HT7receptors) in colonic tissue of chronic diarrhea rats. Materials and Methods: A rat model of chronic diarrhea was established by lactose diet. The expression of 5-HT3, 5-HT4, and 5-HT7receptors in the colonic tissue was detected using immunohistochemistry, real-time PCR and Western blotting techniques. Results: There is no significant difference on the protein expression of 5-HT3receptor between the normal group and the chronic diarrhea model group. The mRNA expression of 5-HT3receptor in the chronic diarrhea model group was significantly lower than that in the normal group (n = 10; P< 0.01). The protein and mRNA expression of 5-HT4receptor in the chronic diarrhea model group were significantly higher than those in the normal group (n = 10; P< 0.05, P< 0.01). On the contrary, the protein and mRNA expressions of 5-HT7receptor in the chronic diarrhea model group were significantly decreased compared with the normal group (n = 10; P< 0.01, P< 0.01). Conclusions: The results suggested the receptors of 5-HT4and 5-HT7may be involved in inducing diarrhea by lactose diet. |
| first_indexed | 2024-12-21T08:08:11Z |
| format | Article |
| id | doaj.art-99de213b514e4846825d00e81d7ff1f4 |
| institution | Directory Open Access Journal |
| issn | 1319-3767 1998-4049 |
| language | English |
| last_indexed | 2024-12-21T08:08:11Z |
| publishDate | 2016-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Saudi Journal of Gastroenterology |
| spelling | doaj.art-99de213b514e4846825d00e81d7ff1f42022-12-21T19:10:44ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492016-01-0122323423910.4103/1319-3767.182460Expression of serotonin receptors in the colonic tissue of chronic diarrhea ratsTong ZhuJuanjuan QiuJiajia WanFengyun WangXudong TangHuishu GuoBackground/Aims: This study aimed to investigate the difference among the expression of serotonin receptors (5-HT3, 5-HT4, and 5-HT7receptors) in colonic tissue of chronic diarrhea rats. Materials and Methods: A rat model of chronic diarrhea was established by lactose diet. The expression of 5-HT3, 5-HT4, and 5-HT7receptors in the colonic tissue was detected using immunohistochemistry, real-time PCR and Western blotting techniques. Results: There is no significant difference on the protein expression of 5-HT3receptor between the normal group and the chronic diarrhea model group. The mRNA expression of 5-HT3receptor in the chronic diarrhea model group was significantly lower than that in the normal group (n = 10; P< 0.01). The protein and mRNA expression of 5-HT4receptor in the chronic diarrhea model group were significantly higher than those in the normal group (n = 10; P< 0.05, P< 0.01). On the contrary, the protein and mRNA expressions of 5-HT7receptor in the chronic diarrhea model group were significantly decreased compared with the normal group (n = 10; P< 0.01, P< 0.01). Conclusions: The results suggested the receptors of 5-HT4and 5-HT7may be involved in inducing diarrhea by lactose diet.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2016;volume=22;issue=3;spage=234;epage=239;aulast=Zhu5-HT35-HT4and 5-HT7 receptorschronic diarrheacolonic tissuelactose |
| spellingShingle | Tong Zhu Juanjuan Qiu Jiajia Wan Fengyun Wang Xudong Tang Huishu Guo Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats The Saudi Journal of Gastroenterology 5-HT3 5-HT4 and 5-HT7 receptors chronic diarrhea colonic tissue lactose |
| title | Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats |
| title_full | Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats |
| title_fullStr | Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats |
| title_full_unstemmed | Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats |
| title_short | Expression of serotonin receptors in the colonic tissue of chronic diarrhea rats |
| title_sort | expression of serotonin receptors in the colonic tissue of chronic diarrhea rats |
| topic | 5-HT3 5-HT4 and 5-HT7 receptors chronic diarrhea colonic tissue lactose |
| url | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2016;volume=22;issue=3;spage=234;epage=239;aulast=Zhu |
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