Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice
To investigate the ameliorative effect of the D-α-tocopheryl acetate compound on D-galactose-induced aging in mice, the in vitro antioxidant capacity of the compound of natural oils+phytosterols (VEO), the compound of D-α-tocopheryl acetate+phytosterol (VEZ), and the compound of D-α-tocopheryl aceta...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-11-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120187 |
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| author | Xutian KAN Weili CHEN Jiaxu LI Wenjiang HE Liugang DING Pan LI Bing DU Wenzhi LI |
| author_facet | Xutian KAN Weili CHEN Jiaxu LI Wenjiang HE Liugang DING Pan LI Bing DU Wenzhi LI |
| author_sort | Xutian KAN |
| collection | DOAJ |
| description | To investigate the ameliorative effect of the D-α-tocopheryl acetate compound on D-galactose-induced aging in mice, the in vitro antioxidant capacity of the compound of natural oils+phytosterols (VEO), the compound of D-α-tocopheryl acetate+phytosterol (VEZ), and the compound of D-α-tocopheryl acetat+phytosterol+astaxanthin (VEX) were measured. The aging model was established using mice injected with D-galactose on the back of the neck, while the intervention was carried out with different compounds. The results showed that all three groups of compounds had strong antioxidant effects, with the VEZ group showing better in vitro antioxidant effects. Compared with the aging model mice, the intervention of the three compounds increased glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), decreased malondialdehyde (MDA) (P<0.01), and a decrease in the serum inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and liver function indicators alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were significantly reduced (P<0.01). After the intervention, the mRNA and protein expression of nuclear factor-erythroid 2-related factor 2 (Nrf2), quinone oxidoreductase (NQO-1) and heme oxygenase-1 (HO-1) in mice were significantly enhanced (P<0.0001). This indicated that the different combinations exerted their antioxidant effects through up-regulating the expression of Nrf2, NQO-1 and HO-1, thus achieving anti-aging effects, with the VEZ group showing the best expression effect. In conclusion, D-α-Tocopheryl acetate complex achieved their anti-aging effects by increasing the expression of antioxidant-related mRNAs and proteins, thus enhancing the levels of downstream antioxidant enzymes, among which D-α-tocopheryl acetate was more effective when combined with phytosterols. |
| first_indexed | 2024-03-11T10:54:19Z |
| format | Article |
| id | doaj.art-99dea2a400494556a08bab85fb80ba23 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T10:54:19Z |
| publishDate | 2023-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-99dea2a400494556a08bab85fb80ba232023-11-13T11:13:53ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-11-01442232733410.13386/j.issn1002-0306.20221201872022120187-22Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in MiceXutian KAN0Weili CHEN1Jiaxu LI2Wenjiang HE3Liugang DING4Pan LI5Bing DU6Wenzhi LI7Research and Development Centre of Infinitus (China) , Guangzhou 510623, ChinaCollege of Food Science, South China Agriculture University, Guangzhou 510642, ChinaCollege of Food Science, South China Agriculture University, Guangzhou 510642, ChinaResearch and Development Centre of Infinitus (China) , Guangzhou 510623, ChinaResearch and Development Centre of Infinitus (China) , Guangzhou 510623, ChinaCollege of Food Science, South China Agriculture University, Guangzhou 510642, ChinaCollege of Food Science, South China Agriculture University, Guangzhou 510642, ChinaResearch and Development Centre of Infinitus (China) , Guangzhou 510623, ChinaTo investigate the ameliorative effect of the D-α-tocopheryl acetate compound on D-galactose-induced aging in mice, the in vitro antioxidant capacity of the compound of natural oils+phytosterols (VEO), the compound of D-α-tocopheryl acetate+phytosterol (VEZ), and the compound of D-α-tocopheryl acetat+phytosterol+astaxanthin (VEX) were measured. The aging model was established using mice injected with D-galactose on the back of the neck, while the intervention was carried out with different compounds. The results showed that all three groups of compounds had strong antioxidant effects, with the VEZ group showing better in vitro antioxidant effects. Compared with the aging model mice, the intervention of the three compounds increased glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC), decreased malondialdehyde (MDA) (P<0.01), and a decrease in the serum inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and liver function indicators alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were significantly reduced (P<0.01). After the intervention, the mRNA and protein expression of nuclear factor-erythroid 2-related factor 2 (Nrf2), quinone oxidoreductase (NQO-1) and heme oxygenase-1 (HO-1) in mice were significantly enhanced (P<0.0001). This indicated that the different combinations exerted their antioxidant effects through up-regulating the expression of Nrf2, NQO-1 and HO-1, thus achieving anti-aging effects, with the VEZ group showing the best expression effect. In conclusion, D-α-Tocopheryl acetate complex achieved their anti-aging effects by increasing the expression of antioxidant-related mRNAs and proteins, thus enhancing the levels of downstream antioxidant enzymes, among which D-α-tocopheryl acetate was more effective when combined with phytosterols.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120187d-α-tocopherol acetated-galactoseantioxidant effectanti-aging effectnuclear factor-e2-related factor (nrf2) |
| spellingShingle | Xutian KAN Weili CHEN Jiaxu LI Wenjiang HE Liugang DING Pan LI Bing DU Wenzhi LI Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice Shipin gongye ke-ji d-α-tocopherol acetate d-galactose antioxidant effect anti-aging effect nuclear factor-e2-related factor (nrf2) |
| title | Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice |
| title_full | Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice |
| title_fullStr | Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice |
| title_full_unstemmed | Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice |
| title_short | Ameliorative Effect of D-α-Tocopherol Acetate Complexes on D-Galactose-Induced Aging in Mice |
| title_sort | ameliorative effect of d α tocopherol acetate complexes on d galactose induced aging in mice |
| topic | d-α-tocopherol acetate d-galactose antioxidant effect anti-aging effect nuclear factor-e2-related factor (nrf2) |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120187 |
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