Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts
Objectives: To prospectively evaluate the performance of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems (MALDI-TOF MS) for the identification of clinically significant yeast isolates compared to the VITEK 2 system. Methods: One hundred and eighty-eight conse...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-09-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971214015604 |
| _version_ | 1823989072333373440 |
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| author | W.Y. Jamal S. Ahmad Z.U. Khan V.O. Rotimi |
| author_facet | W.Y. Jamal S. Ahmad Z.U. Khan V.O. Rotimi |
| author_sort | W.Y. Jamal |
| collection | DOAJ |
| description | Objectives: To prospectively evaluate the performance of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems (MALDI-TOF MS) for the identification of clinically significant yeast isolates compared to the VITEK 2 system.
Methods: One hundred and eighty-eight consecutive yeast isolates were analyzed by Bruker Biotyper and VITEK MS. The results were compared with the conventional VITEK 2 yeast identification system. Discrepant results were resolved by direct sequencing of rDNA.
Results: Accurate identification by VITEK 2, VITEK MS, and Bruker Biotyper MS was 94.1% (177/188), 93.0% (175/188), and 92.6% (174/188), respectively. Three isolates were not identified by VITEK MS, while nine Candida orthopsilosis were misidentified as Candida parapsilosis, as this species is not present in its database. Eleven isolates were not identified or were wrongly identified by Bruker Biotyper and although another 14 were correctly identified, the score was unreliable at <1.7.
Conclusion: The overall accuracy of rapid MALDI-TOF MS systems was essentially comparable to that of the conventional VITEK 2 yeast identification system. However, future expansion of the databases may further improve the outcome and accuracy of identification of yeast species. |
| first_indexed | 2024-12-18T13:53:02Z |
| format | Article |
| id | doaj.art-99e0617aeca04b7f925238781fcd85d3 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-18T13:53:02Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-99e0617aeca04b7f925238781fcd85d32022-12-21T21:05:33ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112014-09-0126C16717010.1016/j.ijid.2014.05.031Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeastsW.Y. Jamal0S. Ahmad1Z.U. Khan2V.O. Rotimi3Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, KuwaitDepartment of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, KuwaitDepartment of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, KuwaitDepartment of Microbiology, Faculty of Medicine, Kuwait University, PO Box 24923, Safat 13110, KuwaitObjectives: To prospectively evaluate the performance of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry systems (MALDI-TOF MS) for the identification of clinically significant yeast isolates compared to the VITEK 2 system. Methods: One hundred and eighty-eight consecutive yeast isolates were analyzed by Bruker Biotyper and VITEK MS. The results were compared with the conventional VITEK 2 yeast identification system. Discrepant results were resolved by direct sequencing of rDNA. Results: Accurate identification by VITEK 2, VITEK MS, and Bruker Biotyper MS was 94.1% (177/188), 93.0% (175/188), and 92.6% (174/188), respectively. Three isolates were not identified by VITEK MS, while nine Candida orthopsilosis were misidentified as Candida parapsilosis, as this species is not present in its database. Eleven isolates were not identified or were wrongly identified by Bruker Biotyper and although another 14 were correctly identified, the score was unreliable at <1.7. Conclusion: The overall accuracy of rapid MALDI-TOF MS systems was essentially comparable to that of the conventional VITEK 2 yeast identification system. However, future expansion of the databases may further improve the outcome and accuracy of identification of yeast species.http://www.sciencedirect.com/science/article/pii/S1201971214015604MALDI-TOF MSYeastsIdentificationVITEK 2 system |
| spellingShingle | W.Y. Jamal S. Ahmad Z.U. Khan V.O. Rotimi Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts International Journal of Infectious Diseases MALDI-TOF MS Yeasts Identification VITEK 2 system |
| title | Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts |
| title_full | Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts |
| title_fullStr | Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts |
| title_full_unstemmed | Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts |
| title_short | Comparative evaluation of two matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems for the identification of clinically significant yeasts |
| title_sort | comparative evaluation of two matrix assisted laser desorption ionization time of flight mass spectrometry maldi tof ms systems for the identification of clinically significant yeasts |
| topic | MALDI-TOF MS Yeasts Identification VITEK 2 system |
| url | http://www.sciencedirect.com/science/article/pii/S1201971214015604 |
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