Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study
This preliminary study investigated pathogenic microbial contamination on fish landed in Ambon City, Indonesia, and evaluated the presence of microbial foodborne pathogens in both fish and environmental samples. The study used a rapid dry sheet media as an in-situ presumptive assay and a PCR method...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/79/e3sconf_isffs2023_04003.pdf |
| _version_ | 1797345569869398016 |
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| author | Triwibowo Radestya Yennie Yusma Widianto Wahyu |
| author_facet | Triwibowo Radestya Yennie Yusma Widianto Wahyu |
| author_sort | Triwibowo Radestya |
| collection | DOAJ |
| description | This preliminary study investigated pathogenic microbial contamination on fish landed in Ambon City, Indonesia, and evaluated the presence of microbial foodborne pathogens in both fish and environmental samples. The study used a rapid dry sheet media as an in-situ presumptive assay and a PCR method as a confirmatory assay. The results indicated the presence of various foodborne pathogenic bacteria, including V. parahaemolyticus, Salmonella spp., and E. coli, in the water and fish basket samples. Three out of thirty-six fish samples were also contaminated with these bacteria. Although the presumptive Listeria assay showed high positive results (36/81), none of them were confirmed as Listeria monocytogenes by PCR assay, indicating that a rapid dry sheet media may not be suitable as an in-situ rapid screening assay for fish and environmental samples. The study highlights the need for better sanitation and hygiene practices, especially at ERI Traditional Fish Landing in Ambon, and may help competent authorities and the seafood industry to implement effective control measures to ensure food safety and public health. |
| first_indexed | 2024-03-08T11:20:37Z |
| format | Article |
| id | doaj.art-99e22184afd44b5299b6010fa2fff490 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-08T11:20:37Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-99e22184afd44b5299b6010fa2fff4902024-01-26T10:02:20ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014420400310.1051/e3sconf/202344204003e3sconf_isffs2023_04003Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary studyTriwibowo Radestya0Yennie Yusma1Widianto Wahyu2Research Center for Food Technology and Processing, Research Organization for Agriculture and Food, National Research and Innovation AgencyResearch Center for Marine and Fisheries Product Processing and Biotechnology, Ministry of Marine Affairs and FisheriesResearch Center for Marine and Fisheries Product Processing and Biotechnology, Ministry of Marine Affairs and FisheriesThis preliminary study investigated pathogenic microbial contamination on fish landed in Ambon City, Indonesia, and evaluated the presence of microbial foodborne pathogens in both fish and environmental samples. The study used a rapid dry sheet media as an in-situ presumptive assay and a PCR method as a confirmatory assay. The results indicated the presence of various foodborne pathogenic bacteria, including V. parahaemolyticus, Salmonella spp., and E. coli, in the water and fish basket samples. Three out of thirty-six fish samples were also contaminated with these bacteria. Although the presumptive Listeria assay showed high positive results (36/81), none of them were confirmed as Listeria monocytogenes by PCR assay, indicating that a rapid dry sheet media may not be suitable as an in-situ rapid screening assay for fish and environmental samples. The study highlights the need for better sanitation and hygiene practices, especially at ERI Traditional Fish Landing in Ambon, and may help competent authorities and the seafood industry to implement effective control measures to ensure food safety and public health.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/79/e3sconf_isffs2023_04003.pdf |
| spellingShingle | Triwibowo Radestya Yennie Yusma Widianto Wahyu Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study E3S Web of Conferences |
| title | Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study |
| title_full | Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study |
| title_fullStr | Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study |
| title_full_unstemmed | Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study |
| title_short | Assessment of pathogenic microbial contamination in fish and environmental samples in Ambon City, Indonesia: A preliminary study |
| title_sort | assessment of pathogenic microbial contamination in fish and environmental samples in ambon city indonesia a preliminary study |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/79/e3sconf_isffs2023_04003.pdf |
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