Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples
The massive production and improper disposal of organohalides resulted in worldwide contamination in soil and water. However, their environmental survey based on chromatographic methods was hindered by challenges in testing the extremely wide variety of organohalides. Dehalococcoides as obligate org...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01677/full |
| _version_ | 1811192472413405184 |
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| author | Qihong Lu Ling Yu Zhiwei Liang Qingyun Yan Zhili He Tiangang Luan Dawei Liang Shanquan Wang Shanquan Wang |
| author_facet | Qihong Lu Ling Yu Zhiwei Liang Qingyun Yan Zhili He Tiangang Luan Dawei Liang Shanquan Wang Shanquan Wang |
| author_sort | Qihong Lu |
| collection | DOAJ |
| description | The massive production and improper disposal of organohalides resulted in worldwide contamination in soil and water. However, their environmental survey based on chromatographic methods was hindered by challenges in testing the extremely wide variety of organohalides. Dehalococcoides as obligate organohalide-respiring bacteria exclusively use organohalides as electron acceptors to support their growth, of which the presence could be coupled with organohalides and, therefore, could be employed as a biomarker of the organohalide pollution. In this study, Dehalococcoides was screened in various samples of bioreactors and subsurface environments, showing the wide distribution of Dehalococcoides in sludge and sediment. Further laboratory cultivation confirmed the dechlorination activities of those Dehalococcoides. Among those samples, Dehalococcoides accounting for 1.8% of the total microbial community was found in an anaerobic granular sludge sample collected from a full-scale bioreactor treating petroleum wastewater. Experimental evidence suggested that the influent wastewater in the bioreactor contained bromomethane which support the growth of Dehalococcoides. This study demonstrated that Dehalococcoides could be employed as a promising biomarker to test the present of organohalides in wastestreams or other environmental samples. |
| first_indexed | 2024-04-11T23:53:08Z |
| format | Article |
| id | doaj.art-99e33e508c1a44b498bef9896334da38 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T23:53:08Z |
| publishDate | 2017-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-99e33e508c1a44b498bef9896334da382022-12-22T03:56:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-09-01810.3389/fmicb.2017.01677296580Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental SamplesQihong Lu0Ling Yu1Zhiwei Liang2Qingyun Yan3Zhili He4Tiangang Luan5Dawei Liang6Shanquan Wang7Shanquan Wang8Environmental Microbiome Research Center and the School of Environmental Science and Engineering, Sun Yat-sen UniversityGuangzhou, ChinaEnvironmental Microbiome Research Center and the School of Environmental Science and Engineering, Sun Yat-sen UniversityGuangzhou, ChinaEnvironmental Microbiome Research Center and the School of Environmental Science and Engineering, Sun Yat-sen UniversityGuangzhou, ChinaEnvironmental Microbiome Research Center and the School of Environmental Science and Engineering, Sun Yat-sen UniversityGuangzhou, ChinaEnvironmental Microbiome Research Center and the School of Environmental Science and Engineering, Sun Yat-sen UniversityGuangzhou, ChinaState Key Laboratory of Pest Control and Resource Utilization, School of Life Sciences, Sun Yat-sen UniversityGuangzhou, ChinaBeijing Key Laboratory of Bio-inspired Energy Materials and Devices, School of Chemistry and Environment, Beihang UniversityBeijing, ChinaEnvironmental Microbiome Research Center and the School of Environmental Science and Engineering, Sun Yat-sen UniversityGuangzhou, ChinaGuangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation TechnologyGuangzhou, ChinaThe massive production and improper disposal of organohalides resulted in worldwide contamination in soil and water. However, their environmental survey based on chromatographic methods was hindered by challenges in testing the extremely wide variety of organohalides. Dehalococcoides as obligate organohalide-respiring bacteria exclusively use organohalides as electron acceptors to support their growth, of which the presence could be coupled with organohalides and, therefore, could be employed as a biomarker of the organohalide pollution. In this study, Dehalococcoides was screened in various samples of bioreactors and subsurface environments, showing the wide distribution of Dehalococcoides in sludge and sediment. Further laboratory cultivation confirmed the dechlorination activities of those Dehalococcoides. Among those samples, Dehalococcoides accounting for 1.8% of the total microbial community was found in an anaerobic granular sludge sample collected from a full-scale bioreactor treating petroleum wastewater. Experimental evidence suggested that the influent wastewater in the bioreactor contained bromomethane which support the growth of Dehalococcoides. This study demonstrated that Dehalococcoides could be employed as a promising biomarker to test the present of organohalides in wastestreams or other environmental samples.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01677/fullDehalococcoidesbiomarkerenvironmental samplesorganohalide compoundsreductive dehalogenation |
| spellingShingle | Qihong Lu Ling Yu Zhiwei Liang Qingyun Yan Zhili He Tiangang Luan Dawei Liang Shanquan Wang Shanquan Wang Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples Frontiers in Microbiology Dehalococcoides biomarker environmental samples organohalide compounds reductive dehalogenation |
| title | Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples |
| title_full | Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples |
| title_fullStr | Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples |
| title_full_unstemmed | Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples |
| title_short | Dehalococcoides as a Potential Biomarker Evidence for Uncharacterized Organohalides in Environmental Samples |
| title_sort | dehalococcoides as a potential biomarker evidence for uncharacterized organohalides in environmental samples |
| topic | Dehalococcoides biomarker environmental samples organohalide compounds reductive dehalogenation |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01677/full |
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