Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus)
Abstract Background Toxoplasma gondii is a worldwide zoonotic protozoan. Consumption of raw milk from infected animals is considered a risk factor for acquiring toxoplasmosis in humans. Recently, donkey milk has been indicated for therapeutic and nutritional purposes and T. gondii infection is commo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2014-04-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/1756-3305-7-165 |
| _version_ | 1797811699555762176 |
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| author | Francesca Mancianti Simona Nardoni Roberto Papini Linda Mugnaini Mina Martini Iolanda Altomonte Federica Salari Carlo D’Ascenzi Jitender P Dubey |
| author_facet | Francesca Mancianti Simona Nardoni Roberto Papini Linda Mugnaini Mina Martini Iolanda Altomonte Federica Salari Carlo D’Ascenzi Jitender P Dubey |
| author_sort | Francesca Mancianti |
| collection | DOAJ |
| description | Abstract Background Toxoplasma gondii is a worldwide zoonotic protozoan. Consumption of raw milk from infected animals is considered a risk factor for acquiring toxoplasmosis in humans. Recently, donkey milk has been indicated for therapeutic and nutritional purposes and T. gondii infection is common in donkeys. The purpose of the present paper was to detect the presence of parasite DNA in milk of T. gondii positive donkeys. Findings Antibodies to T. gondii were found in 11 out of 44 healthy lactating donkeys by IFAT. T. gondii DNA was detected by PCR in blood of 6 and milk of 3 seropositive jennies. Results of limited RFLP-PCR genotyping indicated the presence of T. gondii genotype II or III, commonly found in Europe. Conclusions The occurrence of T. gondii DNA in milk suggests that the consumption of raw milk from seropositive donkeys could be a potential source of human infection. |
| first_indexed | 2024-03-13T07:27:36Z |
| format | Article |
| id | doaj.art-99e395cacf6a4de9887866179a328572 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:36Z |
| publishDate | 2014-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-99e395cacf6a4de9887866179a3285722023-06-04T11:18:08ZengBMCParasites & Vectors1756-33052014-04-01711310.1186/1756-3305-7-165Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus)Francesca Mancianti0Simona Nardoni1Roberto Papini2Linda Mugnaini3Mina Martini4Iolanda Altomonte5Federica Salari6Carlo D’Ascenzi7Jitender P Dubey8Dipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeDipartimento di Scienze Veterinarie, Viale delle PiaggeAbstract Background Toxoplasma gondii is a worldwide zoonotic protozoan. Consumption of raw milk from infected animals is considered a risk factor for acquiring toxoplasmosis in humans. Recently, donkey milk has been indicated for therapeutic and nutritional purposes and T. gondii infection is common in donkeys. The purpose of the present paper was to detect the presence of parasite DNA in milk of T. gondii positive donkeys. Findings Antibodies to T. gondii were found in 11 out of 44 healthy lactating donkeys by IFAT. T. gondii DNA was detected by PCR in blood of 6 and milk of 3 seropositive jennies. Results of limited RFLP-PCR genotyping indicated the presence of T. gondii genotype II or III, commonly found in Europe. Conclusions The occurrence of T. gondii DNA in milk suggests that the consumption of raw milk from seropositive donkeys could be a potential source of human infection.https://doi.org/10.1186/1756-3305-7-165Toxoplasma gondiiDonkeySeroprevalenceMilkGenotypeItaly |
| spellingShingle | Francesca Mancianti Simona Nardoni Roberto Papini Linda Mugnaini Mina Martini Iolanda Altomonte Federica Salari Carlo D’Ascenzi Jitender P Dubey Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus) Parasites & Vectors Toxoplasma gondii Donkey Seroprevalence Milk Genotype Italy |
| title | Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus) |
| title_full | Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus) |
| title_fullStr | Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus) |
| title_full_unstemmed | Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus) |
| title_short | Detection and genotyping of Toxoplasma gondii DNA in the blood and milk of naturally infected donkeys (Equus asinus) |
| title_sort | detection and genotyping of toxoplasma gondii dna in the blood and milk of naturally infected donkeys equus asinus |
| topic | Toxoplasma gondii Donkey Seroprevalence Milk Genotype Italy |
| url | https://doi.org/10.1186/1756-3305-7-165 |
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