Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential
Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2020-11-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/2041731420956541 |
| _version_ | 1818263322068254720 |
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| author | Anna Diez-Escudero Hugo Harlin Per Isaksson Cecilia Persson |
| author_facet | Anna Diez-Escudero Hugo Harlin Per Isaksson Cecilia Persson |
| author_sort | Anna Diez-Escudero |
| collection | DOAJ |
| description | Three different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and Gyroid structures were 3D printed in polylactic acid, a resorbable medical grade material. The 3D printed structures were investigated for printing feasibility, and assessed by morphometric studies. Mechanical properties and permeability investigations resulted in similar values to cancellous bone. The morphometric analyses showed three different patterns of pore distribution: mono-, bi-, and multimodal pores. Subsequently, biological activity investigated with pre-osteoblastic cell lines showed no signs of cytotoxicity, and the scaffolds supported cell proliferation up to 3 weeks. Cell differentiation investigated by alkaline phosphatase showed an improvement for higher porosities and multimodal pore distributions, suggesting a higher dependency on pore distribution and size than the level of interconnectivity. |
| first_indexed | 2024-12-12T19:17:10Z |
| format | Article |
| id | doaj.art-99e430bb1547473aada4d473ee271faf |
| institution | Directory Open Access Journal |
| issn | 2041-7314 |
| language | English |
| last_indexed | 2024-12-12T19:17:10Z |
| publishDate | 2020-11-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj.art-99e430bb1547473aada4d473ee271faf2022-12-22T00:14:42ZengSAGE PublishingJournal of Tissue Engineering2041-73142020-11-011110.1177/2041731420956541Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potentialAnna Diez-Escudero0Hugo Harlin1Per Isaksson2Cecilia Persson3Division of Applied Materials Science, Department of Materials Science and Engineering, Uppsala University, Uppsala, SwedenDivision of Applied Mechanics, Department of Materials Science and Engineering, Uppsala University, Uppsala, SwedenDivision of Applied Mechanics, Department of Materials Science and Engineering, Uppsala University, Uppsala, SwedenDivision of Applied Materials Science, Department of Materials Science and Engineering, Uppsala University, Uppsala, SwedenThree different triply periodic minimal surfaces (TPMS) with three levels of porosity within those of cancellous bone were investigated as potential bone scaffolds. TPMS have emerged as potential designs to resemble the complex mechanical and mass transport properties of bone. Diamond, Schwarz, and Gyroid structures were 3D printed in polylactic acid, a resorbable medical grade material. The 3D printed structures were investigated for printing feasibility, and assessed by morphometric studies. Mechanical properties and permeability investigations resulted in similar values to cancellous bone. The morphometric analyses showed three different patterns of pore distribution: mono-, bi-, and multimodal pores. Subsequently, biological activity investigated with pre-osteoblastic cell lines showed no signs of cytotoxicity, and the scaffolds supported cell proliferation up to 3 weeks. Cell differentiation investigated by alkaline phosphatase showed an improvement for higher porosities and multimodal pore distributions, suggesting a higher dependency on pore distribution and size than the level of interconnectivity.https://doi.org/10.1177/2041731420956541 |
| spellingShingle | Anna Diez-Escudero Hugo Harlin Per Isaksson Cecilia Persson Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential Journal of Tissue Engineering |
| title | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
| title_full | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
| title_fullStr | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
| title_full_unstemmed | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
| title_short | Porous polylactic acid scaffolds for bone regeneration: A study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
| title_sort | porous polylactic acid scaffolds for bone regeneration a study of additively manufactured triply periodic minimal surfaces and their osteogenic potential |
| url | https://doi.org/10.1177/2041731420956541 |
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