Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease
Abstract Background Identification of COPD patients with a rapid decline in FEV1 is of particular interest for prognostic and therapeutic reasons. Objective To determine the expression of markers of inflammation in COPD patients with rapid functional decline in comparison to slow or no decliners. Me...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12931-022-02125-3 |
| _version_ | 1818505142312370176 |
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| author | Antonino Di Stefano Francesca Dossena Isabella Gnemmi Silvestro Ennio D’Anna Paola Brun Bruno Balbi Alessio Piraino Antonio Spanevello Francesco Nucera Vitina Carriero Francesca Bertolini Mauro Maniscalco Ian M. Adcock Gaetano Caramori Fabio L. M. Ricciardolo |
| author_facet | Antonino Di Stefano Francesca Dossena Isabella Gnemmi Silvestro Ennio D’Anna Paola Brun Bruno Balbi Alessio Piraino Antonio Spanevello Francesco Nucera Vitina Carriero Francesca Bertolini Mauro Maniscalco Ian M. Adcock Gaetano Caramori Fabio L. M. Ricciardolo |
| author_sort | Antonino Di Stefano |
| collection | DOAJ |
| description | Abstract Background Identification of COPD patients with a rapid decline in FEV1 is of particular interest for prognostic and therapeutic reasons. Objective To determine the expression of markers of inflammation in COPD patients with rapid functional decline in comparison to slow or no decliners. Methods In COPD patients monitored for at least 3 years (mean ± SD: 5.8 ± 3 years) for lung functional decline, the expression and localization of inflammatory markers was measured in bronchial biopsies of patients with no lung functional decline (FEV1% + 30 ± 43 ml/year, n = 21), slow (FEV1% ml/year, − 40 ± 19, n = 14) and rapid decline (FEV1% ml/year, − 112 ± 53, n = 15) using immunohistochemistry. ELISA test was used for polymeric immunoglobulin receptor (pIgR) quantitation “in vitro”. Results The expression of secretory IgA was significantly reduced in bronchial epithelium (p = 0.011) and plasma cell numbers was significantly reduced in the bronchial lamina propria (p = 0.017) of rapid decliners compared to no decliners. Bronchial inflammatory cell infiltration, CD4, CD8, CD68, CD20, NK, neutrophils, eosinophils, mast cells, pIgR, was not changed in epithelium and lamina propria of rapid decliners compared to other groups. Plasma cells/mm2 correlated positively with scored total IgA in lamina propria of all patients. “In vitro” stimulation of 16HBE cells with LPS (10 μg/ml) and IL-8 (10 ng/ml) induced a significant increase while H2O2 (100 μM) significantly decreased pIgR epithelial expression. Conclusion These data show an impaired humoral immune response in rapid decliners with COPD, marked by reduced epithelial secretory IgA and plasma cell numbers in the bronchial lamina propria. These findings may help in the prognostic stratification and treatment of COPD. |
| first_indexed | 2024-12-10T21:46:46Z |
| format | Article |
| id | doaj.art-99e754ddc55e464b92dedc9b4ae49c18 |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-12-10T21:46:46Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-99e754ddc55e464b92dedc9b4ae49c182022-12-22T01:32:21ZengBMCRespiratory Research1465-993X2022-08-0123111310.1186/s12931-022-02125-3Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary diseaseAntonino Di Stefano0Francesca Dossena1Isabella Gnemmi2Silvestro Ennio D’Anna3Paola Brun4Bruno Balbi5Alessio Piraino6Antonio Spanevello7Francesco Nucera8Vitina Carriero9Francesca Bertolini10Mauro Maniscalco11Ian M. Adcock12Gaetano Caramori13Fabio L. M. Ricciardolo14Divisione di Pneumologia e Laboratorio di Citoimmunopatologia dell’Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri, IRCCS, Respiratory Rehabilitation Unit of Veruno InstituteDivisione di Pneumologia e Laboratorio di Citoimmunopatologia dell’Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri, IRCCS, Respiratory Rehabilitation Unit of Veruno InstituteDivisione di Pneumologia e Laboratorio di Citoimmunopatologia dell’Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri, IRCCS, Respiratory Rehabilitation Unit of Veruno InstituteDivisione di Pneumologia, Istituti Clinici Scientifici Maugeri, SpA, Società Benefit, IRCCSDepartment of Molecular Medicine, Histology Unit, University of PadovaDivisione di Pneumologia e Laboratorio di Citoimmunopatologia dell’Apparato Cardio Respiratorio, Istituti Clinici Scientifici Maugeri, IRCCS, Respiratory Rehabilitation Unit of Veruno InstituteMedical Affairs, Chiesi Italia SpADivisione di Pneumologia, Istituti Clinici Scientifici Maugeri, SpA, Società Benefit, IRCCSPneumologia, Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali (BIOMORF), Università di MessinaDepartment of Clinical and Biological Sciences, Rare Lung Disease Unit and Severe Asthma Centre, San Luigi Gonzaga University Hospital, University of TurinDepartment of Clinical and Biological Sciences, Rare Lung Disease Unit and Severe Asthma Centre, San Luigi Gonzaga University Hospital, University of TurinDivisione di Pneumologia, Istituti Clinici Scientifici Maugeri, SpA, Società Benefit, IRCCSNational Heart and Lung Institute, Imperial College LondonPneumologia, Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali (BIOMORF), Università di MessinaDepartment of Clinical and Biological Sciences, Rare Lung Disease Unit and Severe Asthma Centre, San Luigi Gonzaga University Hospital, University of TurinAbstract Background Identification of COPD patients with a rapid decline in FEV1 is of particular interest for prognostic and therapeutic reasons. Objective To determine the expression of markers of inflammation in COPD patients with rapid functional decline in comparison to slow or no decliners. Methods In COPD patients monitored for at least 3 years (mean ± SD: 5.8 ± 3 years) for lung functional decline, the expression and localization of inflammatory markers was measured in bronchial biopsies of patients with no lung functional decline (FEV1% + 30 ± 43 ml/year, n = 21), slow (FEV1% ml/year, − 40 ± 19, n = 14) and rapid decline (FEV1% ml/year, − 112 ± 53, n = 15) using immunohistochemistry. ELISA test was used for polymeric immunoglobulin receptor (pIgR) quantitation “in vitro”. Results The expression of secretory IgA was significantly reduced in bronchial epithelium (p = 0.011) and plasma cell numbers was significantly reduced in the bronchial lamina propria (p = 0.017) of rapid decliners compared to no decliners. Bronchial inflammatory cell infiltration, CD4, CD8, CD68, CD20, NK, neutrophils, eosinophils, mast cells, pIgR, was not changed in epithelium and lamina propria of rapid decliners compared to other groups. Plasma cells/mm2 correlated positively with scored total IgA in lamina propria of all patients. “In vitro” stimulation of 16HBE cells with LPS (10 μg/ml) and IL-8 (10 ng/ml) induced a significant increase while H2O2 (100 μM) significantly decreased pIgR epithelial expression. Conclusion These data show an impaired humoral immune response in rapid decliners with COPD, marked by reduced epithelial secretory IgA and plasma cell numbers in the bronchial lamina propria. These findings may help in the prognostic stratification and treatment of COPD.https://doi.org/10.1186/s12931-022-02125-3Airway inflammationSustainersFunctional FEV1 declinepIgRIgAPlasma cells |
| spellingShingle | Antonino Di Stefano Francesca Dossena Isabella Gnemmi Silvestro Ennio D’Anna Paola Brun Bruno Balbi Alessio Piraino Antonio Spanevello Francesco Nucera Vitina Carriero Francesca Bertolini Mauro Maniscalco Ian M. Adcock Gaetano Caramori Fabio L. M. Ricciardolo Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease Respiratory Research Airway inflammation Sustainers Functional FEV1 decline pIgR IgA Plasma cells |
| title | Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease |
| title_full | Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease |
| title_fullStr | Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease |
| title_full_unstemmed | Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease |
| title_short | Decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease |
| title_sort | decreased humoral immune response in the bronchi of rapid decliners with chronic obstructive pulmonary disease |
| topic | Airway inflammation Sustainers Functional FEV1 decline pIgR IgA Plasma cells |
| url | https://doi.org/10.1186/s12931-022-02125-3 |
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