PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY
Keratoplasty in patients with recurrent destructive processes in the cornea nowadays remains one of the most serious problems in ophthalmic surgery.Purpose. The analysis of results of penetrating keratoplasty (PK) in patients with destructive processes of the cornea or the corneal graft depending on...
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| Format: | Article |
| Language: | Russian |
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Publishing house "Ophthalmology"
2018-07-01
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| Series: | Офтальмохирургия |
| Subjects: | |
| Online Access: | https://www.ophthalmosurgery.ru/jour/article/view/422 |
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| author | N. A. Gavrilova A. V. Revishchin S. A. Borzenok Y. Y. Kalinnikov E. V. Kovshun A. A. Verzin N. S. Gadzhieva O. E. Tishchenko R. A. Verzin M. A. Kuritsyna G. V. Pavlova |
| author_facet | N. A. Gavrilova A. V. Revishchin S. A. Borzenok Y. Y. Kalinnikov E. V. Kovshun A. A. Verzin N. S. Gadzhieva O. E. Tishchenko R. A. Verzin M. A. Kuritsyna G. V. Pavlova |
| author_sort | N. A. Gavrilova |
| collection | DOAJ |
| description | Keratoplasty in patients with recurrent destructive processes in the cornea nowadays remains one of the most serious problems in ophthalmic surgery.Purpose. The analysis of results of penetrating keratoplasty (PK) in patients with destructive processes of the cornea or the corneal graft depending on the initial level of GDNF and the activity of the MAP kinase cascade of the signaling pathways.Material and methods. The analysis of the results of PK was performed in 23 patients (34 eyes) with acute destructive processes of cornea or the corneal graft in different etiologies. The follow-up period was from 6 months to 2 years. The analysis of the results of treatment was carried out depending on the condition of the corneal graft at various times after PK.The immunohistochemical examination of the biological material (corneal disks of the recipient) was performed using antibodies to GDNF, phospho-ERK1 / 2, phospho-JNK1 / 2, Ki67, GAP43, Bcl2 and Bax.Results. The time of epithelialization of the graft after PK in destructive processes of the cornea was increased in 79% of cases. When the epithelialization of the corneal graft was completed more than 6 and 10 days after PK in the postoperative period persistent erosions of the graft (PEG) with ulceration were formed in 22% and 89% of cases, respectively.The intensity of immunohistochemical reactions with antibodies to GDNF, phospho-ERK1 / 2, phospho-JNK1 / 2 in corneal disks at the epithelialization time of up to 5, 10 days and more eythan 10 days after PK was reduced from moderately positive to weakly positive and negative, respectively.When the time of epithelialization was completed in 6-10 days and there was not PECG, the intensity of reactions with antibodies to GDNF remains moderately positive, phospho-ERK1 / 2, phospho-JNK1 / 2 was lightly positive; when there was PEG the intensity of all reactions was weakly positive.Conclusion. The severity of the destructive pathological process in the cornea and postoperative complications after PK in patients with destructive corneal processes depend on the degree of GDNF deficiency, the decrease in the activity of the MAP kinase cascade of signaling pathways; the density of nerve plexuses in the own corneal tissue or corneal graft, and the activity of apoptosis processes. |
| first_indexed | 2024-04-10T21:16:32Z |
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| id | doaj.art-99e8ae954cad45bdb967f45fd94aba8c |
| institution | Directory Open Access Journal |
| issn | 0235-4160 2312-4970 |
| language | Russian |
| last_indexed | 2024-04-10T21:16:32Z |
| publishDate | 2018-07-01 |
| publisher | Publishing house "Ophthalmology" |
| record_format | Article |
| series | Офтальмохирургия |
| spelling | doaj.art-99e8ae954cad45bdb967f45fd94aba8c2023-01-20T12:34:47ZrusPublishing house "Ophthalmology"Офтальмохирургия0235-41602312-49702018-07-0102364110.25276/0235-4160-2018-2-36-41349PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTYN. A. Gavrilova0A. V. Revishchin1S. A. Borzenok2Y. Y. Kalinnikov3E. V. Kovshun4A. A. Verzin5N. S. Gadzhieva6O. E. Tishchenko7R. A. Verzin8M. A. Kuritsyna9G. V. Pavlova10The A.I. Evdokimov Моscow State University of Medicine and DentistryThe Institute of Gen Biology of the Russian Academy of SciencesThe A.I. Evdokimov Моscow State University of Medicine and Dentistry; The S. Fyodorov Eye Microsurgery Federal State InstitutionThe A.I. Evdokimov Моscow State University of Medicine and DentistryThe S. Fyodorov Eye Microsurgery Federal State InstitutionThe S. Fyodorov Eye Microsurgery Federal State InstitutionThe A.I. Evdokimov Моscow State University of Medicine and DentistryThe A.I. Evdokimov Моscow State University of Medicine and DentistryThe A.I. Evdokimov Моscow State University of Medicine and DentistryThe A.I. Evdokimov Моscow State University of Medicine and DentistryThe Institute of Gen Biology of the Russian Academy of SciencesKeratoplasty in patients with recurrent destructive processes in the cornea nowadays remains one of the most serious problems in ophthalmic surgery.Purpose. The analysis of results of penetrating keratoplasty (PK) in patients with destructive processes of the cornea or the corneal graft depending on the initial level of GDNF and the activity of the MAP kinase cascade of the signaling pathways.Material and methods. The analysis of the results of PK was performed in 23 patients (34 eyes) with acute destructive processes of cornea or the corneal graft in different etiologies. The follow-up period was from 6 months to 2 years. The analysis of the results of treatment was carried out depending on the condition of the corneal graft at various times after PK.The immunohistochemical examination of the biological material (corneal disks of the recipient) was performed using antibodies to GDNF, phospho-ERK1 / 2, phospho-JNK1 / 2, Ki67, GAP43, Bcl2 and Bax.Results. The time of epithelialization of the graft after PK in destructive processes of the cornea was increased in 79% of cases. When the epithelialization of the corneal graft was completed more than 6 and 10 days after PK in the postoperative period persistent erosions of the graft (PEG) with ulceration were formed in 22% and 89% of cases, respectively.The intensity of immunohistochemical reactions with antibodies to GDNF, phospho-ERK1 / 2, phospho-JNK1 / 2 in corneal disks at the epithelialization time of up to 5, 10 days and more eythan 10 days after PK was reduced from moderately positive to weakly positive and negative, respectively.When the time of epithelialization was completed in 6-10 days and there was not PECG, the intensity of reactions with antibodies to GDNF remains moderately positive, phospho-ERK1 / 2, phospho-JNK1 / 2 was lightly positive; when there was PEG the intensity of all reactions was weakly positive.Conclusion. The severity of the destructive pathological process in the cornea and postoperative complications after PK in patients with destructive corneal processes depend on the degree of GDNF deficiency, the decrease in the activity of the MAP kinase cascade of signaling pathways; the density of nerve plexuses in the own corneal tissue or corneal graft, and the activity of apoptosis processes.https://www.ophthalmosurgery.ru/jour/article/view/422penetrating keratoplasty (pk)destructive processes in the corneathe epithelialization of the graftpersistent erosion of the graft (peg) |
| spellingShingle | N. A. Gavrilova A. V. Revishchin S. A. Borzenok Y. Y. Kalinnikov E. V. Kovshun A. A. Verzin N. S. Gadzhieva O. E. Tishchenko R. A. Verzin M. A. Kuritsyna G. V. Pavlova PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY Офтальмохирургия penetrating keratoplasty (pk) destructive processes in the cornea the epithelialization of the graft persistent erosion of the graft (peg) |
| title | PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY |
| title_full | PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY |
| title_fullStr | PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY |
| title_full_unstemmed | PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY |
| title_short | PATHOGENETIC MECHANISMS OF EPITHELIALIZATION AND FORMATION OF PERSISTENT ULCERS OF THE GRAFT IN PATIENTS WITH DESTRUCTIVE CORNEAL PROCESSES AFTER PENETRATING KERATOPLASTY |
| title_sort | pathogenetic mechanisms of epithelialization and formation of persistent ulcers of the graft in patients with destructive corneal processes after penetrating keratoplasty |
| topic | penetrating keratoplasty (pk) destructive processes in the cornea the epithelialization of the graft persistent erosion of the graft (peg) |
| url | https://www.ophthalmosurgery.ru/jour/article/view/422 |
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