Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System
AimWe investigated the expression of the renin–angiotensin system (RAS) by cancer stem cell (CSC) subpopulations we have identified in moderately differentiated lip squamous cell carcinoma (MDLSCC).MethodTen MDLSCC samples underwent 3,3-diaminobenzidine (DAB) and immunofluorescent immunohistochemica...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-06-01
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| Series: | Frontiers in Surgery |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full |
| _version_ | 1818954442379100160 |
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| author | Rachna S. Ram Helen D. Brasch Jonathan C. Dunne Paul F. Davis Swee T. Tan Swee T. Tan Tinte Itinteang |
| author_facet | Rachna S. Ram Helen D. Brasch Jonathan C. Dunne Paul F. Davis Swee T. Tan Swee T. Tan Tinte Itinteang |
| author_sort | Rachna S. Ram |
| collection | DOAJ |
| description | AimWe investigated the expression of the renin–angiotensin system (RAS) by cancer stem cell (CSC) subpopulations we have identified in moderately differentiated lip squamous cell carcinoma (MDLSCC).MethodTen MDLSCC samples underwent 3,3-diaminobenzidine (DAB) and immunofluorescent immunohistochemical (IHC) staining for (pro)renin receptor (PRR), angiotensin-converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1), and receptor 2 (ATIIR2). NanoString analysis and Western blotting (WB) were performed on six MDLSCC samples for gene and protein expression, respectively.ResultsIHC staining showed expression of PRR, ATIIR1, and ATIIR2 on cells within the tumor nests (TNs) and the stroma. ACE was localized to the microvessels within the stroma. WB detected PRR, ACE, and ATIIR2. NanoString analysis confirmed gene expression of PRR, ACE, and ATIIR1.ConclusionComponents of the RAS: PRR, ATIIR1, and ATIIR2 are expressed on two CSC subpopulations in MDLSCC, one within the TNs and the other within the stroma. The endothelium of the microvessels within the stroma expresses ACE. |
| first_indexed | 2024-12-20T10:22:14Z |
| format | Article |
| id | doaj.art-99eb1a3294cb4c9f8f62bb177ad0621d |
| institution | Directory Open Access Journal |
| issn | 2296-875X |
| language | English |
| last_indexed | 2024-12-20T10:22:14Z |
| publishDate | 2017-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Surgery |
| spelling | doaj.art-99eb1a3294cb4c9f8f62bb177ad0621d2022-12-21T19:43:54ZengFrontiers Media S.A.Frontiers in Surgery2296-875X2017-06-01410.3389/fsurg.2017.00030260617Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin SystemRachna S. Ram0Helen D. Brasch1Jonathan C. Dunne2Paul F. Davis3Swee T. Tan4Swee T. Tan5Tinte Itinteang6Gillies McIndoe Research Institute, Wellington, New ZealandGillies McIndoe Research Institute, Wellington, New ZealandGillies McIndoe Research Institute, Wellington, New ZealandGillies McIndoe Research Institute, Wellington, New ZealandGillies McIndoe Research Institute, Wellington, New ZealandWellington Regional Plastic, Maxillofacial and Burns Unit, Hutt Hospital, Wellington, New ZealandGillies McIndoe Research Institute, Wellington, New ZealandAimWe investigated the expression of the renin–angiotensin system (RAS) by cancer stem cell (CSC) subpopulations we have identified in moderately differentiated lip squamous cell carcinoma (MDLSCC).MethodTen MDLSCC samples underwent 3,3-diaminobenzidine (DAB) and immunofluorescent immunohistochemical (IHC) staining for (pro)renin receptor (PRR), angiotensin-converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1), and receptor 2 (ATIIR2). NanoString analysis and Western blotting (WB) were performed on six MDLSCC samples for gene and protein expression, respectively.ResultsIHC staining showed expression of PRR, ATIIR1, and ATIIR2 on cells within the tumor nests (TNs) and the stroma. ACE was localized to the microvessels within the stroma. WB detected PRR, ACE, and ATIIR2. NanoString analysis confirmed gene expression of PRR, ACE, and ATIIR1.ConclusionComponents of the RAS: PRR, ATIIR1, and ATIIR2 are expressed on two CSC subpopulations in MDLSCC, one within the TNs and the other within the stroma. The endothelium of the microvessels within the stroma expresses ACE.http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/fulllipsquamous cell carcinomarenin–angiotensin systemcancer stem cellsoral cavitycancer |
| spellingShingle | Rachna S. Ram Helen D. Brasch Jonathan C. Dunne Paul F. Davis Swee T. Tan Swee T. Tan Tinte Itinteang Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System Frontiers in Surgery lip squamous cell carcinoma renin–angiotensin system cancer stem cells oral cavity cancer |
| title | Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System |
| title_full | Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System |
| title_fullStr | Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System |
| title_full_unstemmed | Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System |
| title_short | Cancer Stem Cells in Moderately Differentiated Lip Squamous Cell Carcinoma Express Components of the Renin–Angiotensin System |
| title_sort | cancer stem cells in moderately differentiated lip squamous cell carcinoma express components of the renin angiotensin system |
| topic | lip squamous cell carcinoma renin–angiotensin system cancer stem cells oral cavity cancer |
| url | http://journal.frontiersin.org/article/10.3389/fsurg.2017.00030/full |
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