(+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells
The bioactive natural compound from marine origin, (+)-aeroplysinin-1, has been shown to exhibit potent anti-inflammatory and anti-angiogenic effects. The aim of the present study was to identify new targets for (+)-aeroplysinin-1 in endothelial cells. The sequential use of 2D-electrophoresis and MA...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/16/9/316 |
| _version_ | 1811305714181734400 |
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| author | Javier A. García-Vilas Beatriz Martínez-Poveda Ana R. Quesada Miguel Ángel Medina |
| author_facet | Javier A. García-Vilas Beatriz Martínez-Poveda Ana R. Quesada Miguel Ángel Medina |
| author_sort | Javier A. García-Vilas |
| collection | DOAJ |
| description | The bioactive natural compound from marine origin, (+)-aeroplysinin-1, has been shown to exhibit potent anti-inflammatory and anti-angiogenic effects. The aim of the present study was to identify new targets for (+)-aeroplysinin-1 in endothelial cells. The sequential use of 2D-electrophoresis and MALDI-TOF-TOF/MS allowed us to identify several differentially expressed proteins. Four of these proteins were involved in redox processes and were validated by Western blot. The effects of (+)-aeroplysinin-1 were further studied by testing the effects of the treatment with this compound on the activity of several anti- and pro-oxidant enzymes, as well as on transcription factors involved in redox homeostasis. Finally, changes in the levels of total reactive oxygen species and mitochondrial membrane potential induced by endothelial cell treatments with (+)-aeroplysinin-1 were also determined. Taken altogether, these findings show that (+)-aeroplysinin-1 has multiple targets involved in endothelial cell redox regulation. |
| first_indexed | 2024-04-13T08:30:19Z |
| format | Article |
| id | doaj.art-99ebc5a071a14ebd9d955bd233512d95 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T08:30:19Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-99ebc5a071a14ebd9d955bd233512d952022-12-22T02:54:16ZengMDPI AGMarine Drugs1660-33972018-09-0116931610.3390/md16090316md16090316(+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial CellsJavier A. García-Vilas0Beatriz Martínez-Poveda1Ana R. Quesada2Miguel Ángel Medina3Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, and IBIMA (Biomedical Research Institute of Málaga), Universidad de Málaga, Andalucía Tech, E-29071 Málaga, SpainDepartamento de Biología Molecular y Bioquímica, Facultad de Ciencias, and IBIMA (Biomedical Research Institute of Málaga), Universidad de Málaga, Andalucía Tech, E-29071 Málaga, SpainDepartamento de Biología Molecular y Bioquímica, Facultad de Ciencias, and IBIMA (Biomedical Research Institute of Málaga), Universidad de Málaga, Andalucía Tech, E-29071 Málaga, SpainDepartamento de Biología Molecular y Bioquímica, Facultad de Ciencias, and IBIMA (Biomedical Research Institute of Málaga), Universidad de Málaga, Andalucía Tech, E-29071 Málaga, SpainThe bioactive natural compound from marine origin, (+)-aeroplysinin-1, has been shown to exhibit potent anti-inflammatory and anti-angiogenic effects. The aim of the present study was to identify new targets for (+)-aeroplysinin-1 in endothelial cells. The sequential use of 2D-electrophoresis and MALDI-TOF-TOF/MS allowed us to identify several differentially expressed proteins. Four of these proteins were involved in redox processes and were validated by Western blot. The effects of (+)-aeroplysinin-1 were further studied by testing the effects of the treatment with this compound on the activity of several anti- and pro-oxidant enzymes, as well as on transcription factors involved in redox homeostasis. Finally, changes in the levels of total reactive oxygen species and mitochondrial membrane potential induced by endothelial cell treatments with (+)-aeroplysinin-1 were also determined. Taken altogether, these findings show that (+)-aeroplysinin-1 has multiple targets involved in endothelial cell redox regulation.http://www.mdpi.com/1660-3397/16/9/316aeroplysinin-1angiogenesisAplysina aerophobaendothelial cellredox |
| spellingShingle | Javier A. García-Vilas Beatriz Martínez-Poveda Ana R. Quesada Miguel Ángel Medina (+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells Marine Drugs aeroplysinin-1 angiogenesis Aplysina aerophoba endothelial cell redox |
| title | (+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells |
| title_full | (+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells |
| title_fullStr | (+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells |
| title_full_unstemmed | (+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells |
| title_short | (+)-Aeroplysinin-1 Modulates the Redox Balance of Endothelial Cells |
| title_sort | aeroplysinin 1 modulates the redox balance of endothelial cells |
| topic | aeroplysinin-1 angiogenesis Aplysina aerophoba endothelial cell redox |
| url | http://www.mdpi.com/1660-3397/16/9/316 |
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