Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats
Background: Available evidence suggests that 50% of couples with infertility are male related. Over 40% of these males consume alcohol which has been reported to be a reproductive toxicant causing depletions in the epithelium of seminiferous tubules hence reducing sperm counts and sperm morphology....
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750015300184 |
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| author | E.N. Akang A.A. Oremosu A.A. Osinubi O.O. Dosumu T.O. Kusemiju S.A. Adelakun M.L. Umaru |
| author_facet | E.N. Akang A.A. Oremosu A.A. Osinubi O.O. Dosumu T.O. Kusemiju S.A. Adelakun M.L. Umaru |
| author_sort | E.N. Akang |
| collection | DOAJ |
| description | Background: Available evidence suggests that 50% of couples with infertility are male related. Over 40% of these males consume alcohol which has been reported to be a reproductive toxicant causing depletions in the epithelium of seminiferous tubules hence reducing sperm counts and sperm morphology.
Objective: To determine the effects of aqueous leaf extract of Telfairia occidentalis on alcohol-induced cyto-architectural changes in the testis.
Methods: Aqueous leaf extract of Telfairia occidentalis (T. occidentalis) was administered by gastric gavage at a dose of 250 mg/kg and 500 mg/kg body weight daily, while 2 g/kg body weight of ethanol at 30% v/v was administered daily to mature male Sprague–Dawley rats. The experiment was in 2 phases. Phase 1 had groups A1–F1 and lasted for 4 weeks while phase 2 had groups A2–F2 and lasted 8 weeks. Parameters tested include: testicular histology, relative volume density, sperm parameters, malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione.
Results: In both phases, there were depletions in the seminiferous epithelium, decreased sperm quality and increased MDA and SOD in animals that received alcohol only compared to control. Likewise, a significant increase of seminiferous epithelium of animals that received respective doses of 250 mg/kg and 500 mg/kg of T. occidentalis only compared to control. Animals that received T. occidentalis and alcohol simultaneously had a significant increase in seminiferous epithelium and sperm quality with decreased MDA level.
Conclusion: T. occidentalis attenuated the deleterious effects of alcohol to the cyto-architecture of the testis, protected the seminiferous epithelium, reduced oxidative stress and promoted spermatogenesis. |
| first_indexed | 2024-12-23T19:46:26Z |
| format | Article |
| id | doaj.art-99ec5ebf14c348fabf86bdbd12756b4e |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-23T19:46:26Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-99ec5ebf14c348fabf86bdbd12756b4e2022-12-21T17:33:31ZengElsevierToxicology Reports2214-75002015-01-012C96897510.1016/j.toxrep.2015.06.009Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male ratsE.N. Akang0A.A. Oremosu1A.A. Osinubi2O.O. Dosumu3T.O. Kusemiju4S.A. Adelakun5M.L. Umaru6Department of Anatomy, College of Health Sciences, Benue State University, Makurdi, Benue State, NigeriaDepartment of Anatomy, College of Medicine, University of Lagos, Lagos State, NigeriaDepartment of Anatomy, College of Medicine, University of Lagos, Lagos State, NigeriaDepartment of Anatomy, College of Medicine, University of Lagos, Lagos State, NigeriaDepartment of Anatomy, College of Medicine, University of Lagos, Lagos State, NigeriaDepartment of Anatomy, College of Medicine, University of Lagos, Lagos State, NigeriaDepartment of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, NigeriaBackground: Available evidence suggests that 50% of couples with infertility are male related. Over 40% of these males consume alcohol which has been reported to be a reproductive toxicant causing depletions in the epithelium of seminiferous tubules hence reducing sperm counts and sperm morphology. Objective: To determine the effects of aqueous leaf extract of Telfairia occidentalis on alcohol-induced cyto-architectural changes in the testis. Methods: Aqueous leaf extract of Telfairia occidentalis (T. occidentalis) was administered by gastric gavage at a dose of 250 mg/kg and 500 mg/kg body weight daily, while 2 g/kg body weight of ethanol at 30% v/v was administered daily to mature male Sprague–Dawley rats. The experiment was in 2 phases. Phase 1 had groups A1–F1 and lasted for 4 weeks while phase 2 had groups A2–F2 and lasted 8 weeks. Parameters tested include: testicular histology, relative volume density, sperm parameters, malondialdehyde (MDA), superoxide dismutase (SOD) and reduced glutathione. Results: In both phases, there were depletions in the seminiferous epithelium, decreased sperm quality and increased MDA and SOD in animals that received alcohol only compared to control. Likewise, a significant increase of seminiferous epithelium of animals that received respective doses of 250 mg/kg and 500 mg/kg of T. occidentalis only compared to control. Animals that received T. occidentalis and alcohol simultaneously had a significant increase in seminiferous epithelium and sperm quality with decreased MDA level. Conclusion: T. occidentalis attenuated the deleterious effects of alcohol to the cyto-architecture of the testis, protected the seminiferous epithelium, reduced oxidative stress and promoted spermatogenesis.http://www.sciencedirect.com/science/article/pii/S2214750015300184SpermatogenesisTestisOxidative stressSperm qualityAlcoholTelfairia occidentalis |
| spellingShingle | E.N. Akang A.A. Oremosu A.A. Osinubi O.O. Dosumu T.O. Kusemiju S.A. Adelakun M.L. Umaru Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats Toxicology Reports Spermatogenesis Testis Oxidative stress Sperm quality Alcohol Telfairia occidentalis |
| title | Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats |
| title_full | Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats |
| title_fullStr | Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats |
| title_full_unstemmed | Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats |
| title_short | Histomorphometric studies of the effects of Telfairia occidentalis on alcohol-induced gonado-toxicity in male rats |
| title_sort | histomorphometric studies of the effects of telfairia occidentalis on alcohol induced gonado toxicity in male rats |
| topic | Spermatogenesis Testis Oxidative stress Sperm quality Alcohol Telfairia occidentalis |
| url | http://www.sciencedirect.com/science/article/pii/S2214750015300184 |
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