Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023
Until 2020, Djiboutian health authorities relied on histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) to establish the diagnosis of <i>Plasmodium falciparum</i>. The rapid spread of <i>P. falciparum</i> histidine-rich protein-2 and -3 (<i>pfhrp2/3</...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/14/3/262 |
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| author | Rahma Abdi Moussa Nasserdine Papa Mze Houssein Yonis Arreh Aicha Abdillahi Hamoud Kahiya Mohamed Alaleh Fatouma Mohamed Aden Abdoul-Razak Yonis Omar Warsama Osman Abdi Samatar Kayad Guelleh Abdoul-Ilah Ahmed Abdi Leonardo K. Basco Bouh Abdi Khaireh Hervé Bogreau |
| author_facet | Rahma Abdi Moussa Nasserdine Papa Mze Houssein Yonis Arreh Aicha Abdillahi Hamoud Kahiya Mohamed Alaleh Fatouma Mohamed Aden Abdoul-Razak Yonis Omar Warsama Osman Abdi Samatar Kayad Guelleh Abdoul-Ilah Ahmed Abdi Leonardo K. Basco Bouh Abdi Khaireh Hervé Bogreau |
| author_sort | Rahma Abdi Moussa |
| collection | DOAJ |
| description | Until 2020, Djiboutian health authorities relied on histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) to establish the diagnosis of <i>Plasmodium falciparum</i>. The rapid spread of <i>P. falciparum</i> histidine-rich protein-2 and -3 (<i>pfhrp2/3</i>) gene-deleted parasite strains in Djibouti has led the authorities to switch from HRP2-based RDTs to lactate dehydrogenase (LDH)-based RDTs targeting the plasmodial lactate dehydrogenase (pLDH) specific for <i>P. falciparum</i> and <i>P. vivax</i> (RapiGEN BIOCREDIT Malaria Ag Pf/Pv pLDH/pLDH) in 2021. This study was conducted with the primary objective of evaluating the diagnostic performance of this alternative RDT. Operational constraints related, in particular, to the implementation of this RDT during the COVID-19 pandemic were also considered. The performance of BIOCREDIT Malaria Ag Pf/Pv (pLDH/pLDH) RDT was also compared to our previously published data on the performance of two HRP2-based RDTs deployed in Djibouti in 2018–2020. The diagnosis of 350 febrile patients with suspected malaria in Djibouti city was established using two batches of RapiGEN BIOCREDIT Malaria Ag Pf/Pv (pLDH/pLDH) RDT over a two-year period (2022 and 2023) and confirmed by real-time quantitative polymerase chain reaction. The sensitivity and specificity for the detection of <i>P. falciparum</i> were 88.2% and 100%, respectively. For <i>P. vivax</i>, the sensitivity was 86.7% and the specificity was 100%. Re-training and closer supervision of the technicians between 2022 and 2023 have led to an increased sensitivity to detect <i>P. falciparum</i> (69.8% in 2022 versus 88.2% in 2023; <i>p</i> < 0.01). The receiver operating characteristic curve analysis highlighted a better performance in the diagnosis of <i>P. falciparum</i> with pLDH-based RDTs compared with previous HRP2-based RDTs. In Djibouti, where <i>pfhrp2</i>-deleted strains are rapidly gaining ground, LDH-based RDTs seem to be more suitable for diagnosing <i>P. falciparum</i> than HRP2-based RDTs. Awareness-raising and training for technical staff have also been beneficial. |
| first_indexed | 2024-03-08T03:58:48Z |
| format | Article |
| id | doaj.art-99f14d0aa4c44bbb881d64b0ba6cfc51 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-08T03:58:48Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-99f14d0aa4c44bbb881d64b0ba6cfc512024-02-09T15:10:01ZengMDPI AGDiagnostics2075-44182024-01-0114326210.3390/diagnostics14030262Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023Rahma Abdi Moussa0Nasserdine Papa Mze1Houssein Yonis Arreh2Aicha Abdillahi Hamoud3Kahiya Mohamed Alaleh4Fatouma Mohamed Aden5Abdoul-Razak Yonis Omar6Warsama Osman Abdi7Samatar Kayad Guelleh8Abdoul-Ilah Ahmed Abdi9Leonardo K. Basco10Bouh Abdi Khaireh11Hervé Bogreau12Aix Marseille Université, IRD, AP-HM, SSA, VITROME, 13005 Marseille, FranceAix Marseille Université, IRD, AP-HM, SSA, VITROME, 13005 Marseille, FranceLaboratoire National de Référence, Hôpital Peltier, Ministère de la Santé, Djibouti ville 98230, DjiboutiCaisse Nationale de Sécurité Sociale (CNSS), Djibouti ville 98230, DjiboutiCaisse Nationale de Sécurité Sociale (CNSS), Djibouti ville 98230, DjiboutiCaisse Nationale de Sécurité Sociale (CNSS), Djibouti ville 98230, DjiboutiLaboratoire de Diagnostic, Centre de Santé Communautaire d’Einguela, Ministère de la Santé, Djibouti ville 98230, DjiboutiCaisse Nationale de Sécurité Sociale (CNSS), Djibouti ville 98230, DjiboutiProgramme National de Lutte Contre le Paludisme, Ministère de la Santé, Djibouti ville 98230, DjiboutiService de Santé des Armées, Présidence de la République, Djibouti ville 98230, DjiboutiAix Marseille Université, IRD, AP-HM, SSA, VITROME, 13005 Marseille, FranceUNDP Djibouti, Global Fund to Fight AIDS-TB-Malaria, Djibouti ville 98230, DjiboutiAix Marseille Université, IRD, AP-HM, SSA, VITROME, 13005 Marseille, FranceUntil 2020, Djiboutian health authorities relied on histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) to establish the diagnosis of <i>Plasmodium falciparum</i>. The rapid spread of <i>P. falciparum</i> histidine-rich protein-2 and -3 (<i>pfhrp2/3</i>) gene-deleted parasite strains in Djibouti has led the authorities to switch from HRP2-based RDTs to lactate dehydrogenase (LDH)-based RDTs targeting the plasmodial lactate dehydrogenase (pLDH) specific for <i>P. falciparum</i> and <i>P. vivax</i> (RapiGEN BIOCREDIT Malaria Ag Pf/Pv pLDH/pLDH) in 2021. This study was conducted with the primary objective of evaluating the diagnostic performance of this alternative RDT. Operational constraints related, in particular, to the implementation of this RDT during the COVID-19 pandemic were also considered. The performance of BIOCREDIT Malaria Ag Pf/Pv (pLDH/pLDH) RDT was also compared to our previously published data on the performance of two HRP2-based RDTs deployed in Djibouti in 2018–2020. The diagnosis of 350 febrile patients with suspected malaria in Djibouti city was established using two batches of RapiGEN BIOCREDIT Malaria Ag Pf/Pv (pLDH/pLDH) RDT over a two-year period (2022 and 2023) and confirmed by real-time quantitative polymerase chain reaction. The sensitivity and specificity for the detection of <i>P. falciparum</i> were 88.2% and 100%, respectively. For <i>P. vivax</i>, the sensitivity was 86.7% and the specificity was 100%. Re-training and closer supervision of the technicians between 2022 and 2023 have led to an increased sensitivity to detect <i>P. falciparum</i> (69.8% in 2022 versus 88.2% in 2023; <i>p</i> < 0.01). The receiver operating characteristic curve analysis highlighted a better performance in the diagnosis of <i>P. falciparum</i> with pLDH-based RDTs compared with previous HRP2-based RDTs. In Djibouti, where <i>pfhrp2</i>-deleted strains are rapidly gaining ground, LDH-based RDTs seem to be more suitable for diagnosing <i>P. falciparum</i> than HRP2-based RDTs. Awareness-raising and training for technical staff have also been beneficial.https://www.mdpi.com/2075-4418/14/3/262diagnosisdiagnostic performanceDjiboutihistidine-rich proteinlactate dehydrogenase<i>Plasmodium falciparum</i> |
| spellingShingle | Rahma Abdi Moussa Nasserdine Papa Mze Houssein Yonis Arreh Aicha Abdillahi Hamoud Kahiya Mohamed Alaleh Fatouma Mohamed Aden Abdoul-Razak Yonis Omar Warsama Osman Abdi Samatar Kayad Guelleh Abdoul-Ilah Ahmed Abdi Leonardo K. Basco Bouh Abdi Khaireh Hervé Bogreau Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023 Diagnostics diagnosis diagnostic performance Djibouti histidine-rich protein lactate dehydrogenase <i>Plasmodium falciparum</i> |
| title | Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023 |
| title_full | Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023 |
| title_fullStr | Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023 |
| title_full_unstemmed | Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023 |
| title_short | Assessment of the Performance of Lactate Dehydrogenase-Based Rapid Diagnostic Test for Malaria in Djibouti in 2022–2023 |
| title_sort | assessment of the performance of lactate dehydrogenase based rapid diagnostic test for malaria in djibouti in 2022 2023 |
| topic | diagnosis diagnostic performance Djibouti histidine-rich protein lactate dehydrogenase <i>Plasmodium falciparum</i> |
| url | https://www.mdpi.com/2075-4418/14/3/262 |
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