OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD
The cellulase production with Penicillium nalgiovense S11 on wheat pollard was enhanced using substrate pretreatments, i.e.: (i) mechanic process by Wiley milling, (ii) reducing sugars removal by water soaking, and (iii) chemical pretrcatment by 0.5% NaOH soaking at 100°C. The enzyme production sta...
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
2004-01-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Subjects: | |
| Online Access: | http://journal.biotrop.org/index.php/biotropia/article/view/202/171 |
| _version_ | 1811263704115707904 |
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| author | AGNES T. KUMALASARI TRESNAWATI PURWADARIA PIUS P. KETAREN TUTIHARYATI ARNOLD P. SINURAT |
| author_facet | AGNES T. KUMALASARI TRESNAWATI PURWADARIA PIUS P. KETAREN TUTIHARYATI ARNOLD P. SINURAT |
| author_sort | AGNES T. KUMALASARI |
| collection | DOAJ |
| description | The cellulase production with Penicillium nalgiovense S11 on wheat pollard was enhanced using substrate pretreatments, i.e.: (i) mechanic process by Wiley milling, (ii) reducing sugars removal by water soaking, and (iii) chemical pretrcatment by 0.5% NaOH soaking at 100°C. The enzyme production stated as enzyme activities of all prctrcated substrates were higher than the untreated substrate. Although soaking with water showed significant increase in enzyme activities, the highest CMCase (EC 3.2.1.4), FPase (filter papcrase) and p-glucosidase (EC 3.2.1.21) were observed on NaOH pretreated pollard. The NaOH prctreatment also enhanced the enzyme production by increasing substrate concentration from 2 to 4%. The op timal incubation time in the cellulase production on 4% NaOH-pretrcated pollard was observed on the fifth day. Add ition of 250 ppm glucose also increased the enzyme activities. The optimal treatments increased the specific activities of CMCase, FPase, and |3-glucosidase into 60, 4, and 198 times, respectively, as compared to the specific activities on 2% unpretreated pollard. |
| first_indexed | 2024-04-12T19:49:58Z |
| format | Article |
| id | doaj.art-99f2beda8d904d759d14fd298dbf5b64 |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-04-12T19:49:58Z |
| publishDate | 2004-01-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-99f2beda8d904d759d14fd298dbf5b642022-12-22T03:18:52ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2004-01-0123112OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARDAGNES T. KUMALASARITRESNAWATI PURWADARIAPIUS P. KETARENTUTIHARYATIARNOLD P. SINURATThe cellulase production with Penicillium nalgiovense S11 on wheat pollard was enhanced using substrate pretreatments, i.e.: (i) mechanic process by Wiley milling, (ii) reducing sugars removal by water soaking, and (iii) chemical pretrcatment by 0.5% NaOH soaking at 100°C. The enzyme production stated as enzyme activities of all prctrcated substrates were higher than the untreated substrate. Although soaking with water showed significant increase in enzyme activities, the highest CMCase (EC 3.2.1.4), FPase (filter papcrase) and p-glucosidase (EC 3.2.1.21) were observed on NaOH pretreated pollard. The NaOH prctreatment also enhanced the enzyme production by increasing substrate concentration from 2 to 4%. The op timal incubation time in the cellulase production on 4% NaOH-pretrcated pollard was observed on the fifth day. Add ition of 250 ppm glucose also increased the enzyme activities. The optimal treatments increased the specific activities of CMCase, FPase, and |3-glucosidase into 60, 4, and 198 times, respectively, as compared to the specific activities on 2% unpretreated pollard.http://journal.biotrop.org/index.php/biotropia/article/view/202/171Cellulase production/ pollard pretreatme nts/ water soaking/ NaOH soaking |
| spellingShingle | AGNES T. KUMALASARI TRESNAWATI PURWADARIA PIUS P. KETAREN TUTIHARYATI ARNOLD P. SINURAT OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD Biotropia: The Southeast Asian Journal of Tropical Biology Cellulase production/ pollard pretreatme nts/ water soaking/ NaOH soaking |
| title | OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD |
| title_full | OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD |
| title_fullStr | OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD |
| title_full_unstemmed | OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD |
| title_short | OPTIMIZATION OF CELLULASE PRODUCTION WITH PENICILLIUM NALGIOVENSE Sll GROWN ON PRETREATED WHEAT POLLARD |
| title_sort | optimization of cellulase production with penicillium nalgiovense sll grown on pretreated wheat pollard |
| topic | Cellulase production/ pollard pretreatme nts/ water soaking/ NaOH soaking |
| url | http://journal.biotrop.org/index.php/biotropia/article/view/202/171 |
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