A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver
During prenatal life, the foetal liver is colonised by several waves of haematopoietic progenitors to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression patterns as haemato...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Journal of Developmental Biology |
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| Online Access: | https://www.mdpi.com/2221-3759/11/2/15 |
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| author | Elena Ceccacci Emanuela Villa Fabio Santoro Saverio Minucci Christiana Ruhrberg Alessandro Fantin |
| author_facet | Elena Ceccacci Emanuela Villa Fabio Santoro Saverio Minucci Christiana Ruhrberg Alessandro Fantin |
| author_sort | Elena Ceccacci |
| collection | DOAJ |
| description | During prenatal life, the foetal liver is colonised by several waves of haematopoietic progenitors to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression patterns as haematopoietic development proceeds. To obtain a refined single cell landscape of haematopoiesis in the foetal liver, we have generated a scRNA-seq dataset from a whole mouse E12.5 liver that includes a larger number of cells than prior datasets at this stage and was obtained without cell type preselection to include all liver cell populations. We combined mining of this dataset with that of previously published datasets at other developmental stages to follow transcriptional dynamics as well as the cell cycle state of developing haematopoietic lineages. Our findings corroborate several prior reports on the timing of liver colonisation by haematopoietic progenitors and the emergence of differentiated lineages and provide further molecular characterisation of each cell population. Extending these findings, we demonstrate the existence of a foetal intermediate haemoglobin profile in the mouse, similar to that previously identified in humans, and a previously unidentified population of primitive erythroid cells in the foetal liver. |
| first_indexed | 2024-03-11T02:18:35Z |
| format | Article |
| id | doaj.art-99f3cf3dd2824fc58e6b0cef3105543c |
| institution | Directory Open Access Journal |
| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-03-11T02:18:35Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Developmental Biology |
| spelling | doaj.art-99f3cf3dd2824fc58e6b0cef3105543c2023-11-18T11:02:48ZengMDPI AGJournal of Developmental Biology2221-37592023-03-011121510.3390/jdb11020015A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal LiverElena Ceccacci0Emanuela Villa1Fabio Santoro2Saverio Minucci3Christiana Ruhrberg4Alessandro Fantin5Department of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, ItalyDepartment of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, ItalyDepartment of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, ItalyDepartment of Experimental Oncology, IEO, European Institute of Oncology IRCCS, Via Adamello 16, 20139 Milan, ItalyUCL Institute of Ophthalmology, University College London, 11-43 Bath Street, London EC1V 9EL, UKDepartment of Biosciences, University of Milan, Via G. Celoria 26, 20133 Milan, ItalyDuring prenatal life, the foetal liver is colonised by several waves of haematopoietic progenitors to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression patterns as haematopoietic development proceeds. To obtain a refined single cell landscape of haematopoiesis in the foetal liver, we have generated a scRNA-seq dataset from a whole mouse E12.5 liver that includes a larger number of cells than prior datasets at this stage and was obtained without cell type preselection to include all liver cell populations. We combined mining of this dataset with that of previously published datasets at other developmental stages to follow transcriptional dynamics as well as the cell cycle state of developing haematopoietic lineages. Our findings corroborate several prior reports on the timing of liver colonisation by haematopoietic progenitors and the emergence of differentiated lineages and provide further molecular characterisation of each cell population. Extending these findings, we demonstrate the existence of a foetal intermediate haemoglobin profile in the mouse, similar to that previously identified in humans, and a previously unidentified population of primitive erythroid cells in the foetal liver.https://www.mdpi.com/2221-3759/11/2/15foetal liverhaematopoietic developmenthaemoglobin |
| spellingShingle | Elena Ceccacci Emanuela Villa Fabio Santoro Saverio Minucci Christiana Ruhrberg Alessandro Fantin A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver Journal of Developmental Biology foetal liver haematopoietic development haemoglobin |
| title | A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver |
| title_full | A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver |
| title_fullStr | A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver |
| title_full_unstemmed | A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver |
| title_short | A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver |
| title_sort | refined single cell landscape of haematopoiesis in the mouse foetal liver |
| topic | foetal liver haematopoietic development haemoglobin |
| url | https://www.mdpi.com/2221-3759/11/2/15 |
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