Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor
Wine strains of <i>Saccharomyces</i> <i>cerevisiae</i> have to adapt their metabolism to the changing conditions during their biotechnological use, from the aerobic growth in sucrose-rich molasses for biomass propagation to the anaerobic fermentation of monosaccharides of gra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/3/181 |
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| author | Aroa Ferrer-Pinós Víctor Garrigós Emilia Matallana Agustín Aranda |
| author_facet | Aroa Ferrer-Pinós Víctor Garrigós Emilia Matallana Agustín Aranda |
| author_sort | Aroa Ferrer-Pinós |
| collection | DOAJ |
| description | Wine strains of <i>Saccharomyces</i> <i>cerevisiae</i> have to adapt their metabolism to the changing conditions during their biotechnological use, from the aerobic growth in sucrose-rich molasses for biomass propagation to the anaerobic fermentation of monosaccharides of grape juice during winemaking. Yeast have molecular mechanisms that favor the use of preferred carbon and nitrogen sources to achieve such adaptation. By using specific inhibitors, it was determined that commercial strains offer a wide variety of glucose repression profiles. Transcription factor Gln3 has been involved in glucose and nitrogen repression. Deletion of <i>GLN3</i> in two commercial wine strains produced different mutant phenotypes and only one of them displayed higher glucose repression and was unable to grow using a respiratory carbon source. Therefore, the role of this transcription factor contributes to the variety of phenotypic behaviors seen in wine strains. This variability is also reflected in the impact of <i>GLN3</i> deletion in fermentation, although the mutants are always more tolerant to inhibition of the nutrient signaling complex TORC1 by rapamycin, both in laboratory medium and in grape juice fermentation. Therefore, most aspects of nitrogen catabolite repression controlled by TORC1 are conserved in winemaking conditions. |
| first_indexed | 2024-03-10T07:41:46Z |
| format | Article |
| id | doaj.art-99f6183c8f1146a3bc219953006d044b |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T07:41:46Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-99f6183c8f1146a3bc219953006d044b2023-11-22T13:00:21ZengMDPI AGFermentation2311-56372021-09-017318110.3390/fermentation7030181Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription FactorAroa Ferrer-Pinós0Víctor Garrigós1Emilia Matallana2Agustín Aranda3Institute for Integrative Systems Biology (I2SysBio), University of Valencia-CSIC, 46980 Paterna, SpainInstitute for Integrative Systems Biology (I2SysBio), University of Valencia-CSIC, 46980 Paterna, SpainInstitute for Integrative Systems Biology (I2SysBio), University of Valencia-CSIC, 46980 Paterna, SpainInstitute for Integrative Systems Biology (I2SysBio), University of Valencia-CSIC, 46980 Paterna, SpainWine strains of <i>Saccharomyces</i> <i>cerevisiae</i> have to adapt their metabolism to the changing conditions during their biotechnological use, from the aerobic growth in sucrose-rich molasses for biomass propagation to the anaerobic fermentation of monosaccharides of grape juice during winemaking. Yeast have molecular mechanisms that favor the use of preferred carbon and nitrogen sources to achieve such adaptation. By using specific inhibitors, it was determined that commercial strains offer a wide variety of glucose repression profiles. Transcription factor Gln3 has been involved in glucose and nitrogen repression. Deletion of <i>GLN3</i> in two commercial wine strains produced different mutant phenotypes and only one of them displayed higher glucose repression and was unable to grow using a respiratory carbon source. Therefore, the role of this transcription factor contributes to the variety of phenotypic behaviors seen in wine strains. This variability is also reflected in the impact of <i>GLN3</i> deletion in fermentation, although the mutants are always more tolerant to inhibition of the nutrient signaling complex TORC1 by rapamycin, both in laboratory medium and in grape juice fermentation. Therefore, most aspects of nitrogen catabolite repression controlled by TORC1 are conserved in winemaking conditions.https://www.mdpi.com/2311-5637/7/3/181wine<i>Saccharomyces cerevisiae</i>glucose repressionGln3nitrogen catabolite repression |
| spellingShingle | Aroa Ferrer-Pinós Víctor Garrigós Emilia Matallana Agustín Aranda Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor Fermentation wine <i>Saccharomyces cerevisiae</i> glucose repression Gln3 nitrogen catabolite repression |
| title | Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor |
| title_full | Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor |
| title_fullStr | Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor |
| title_full_unstemmed | Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor |
| title_short | Mechanisms of Metabolic Adaptation in Wine Yeasts: Role of Gln3 Transcription Factor |
| title_sort | mechanisms of metabolic adaptation in wine yeasts role of gln3 transcription factor |
| topic | wine <i>Saccharomyces cerevisiae</i> glucose repression Gln3 nitrogen catabolite repression |
| url | https://www.mdpi.com/2311-5637/7/3/181 |
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